Efficacy of different formulations of nystatin in an experimental model of oral candidiasis in sialoadenectomized rats
Background/purpose: Oral candidiasis is the most common fungal infection of the oral cavity and has become a focus of attention in recent years because of its association with highly topical immunosuppressive conditions. The aims of this study were to determine the value of microbiological, clinical...
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| Format: | Article |
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Elsevier
2021-01-01
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| Series: | Journal of Dental Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1991790220301513 |
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| author | Mario Pérez-Sayáns Renee Beiro-Fuentes Eva M. Otero-Rey Cintia Micaela Chamorro-Petronacci Pilar Gándara-Vila Jose Manuel Somoza-Martín Abel García-García Andrés Blanco-Carrión |
| author_facet | Mario Pérez-Sayáns Renee Beiro-Fuentes Eva M. Otero-Rey Cintia Micaela Chamorro-Petronacci Pilar Gándara-Vila Jose Manuel Somoza-Martín Abel García-García Andrés Blanco-Carrión |
| author_sort | Mario Pérez-Sayáns |
| collection | DOAJ |
| description | Background/purpose: Oral candidiasis is the most common fungal infection of the oral cavity and has become a focus of attention in recent years because of its association with highly topical immunosuppressive conditions. The aims of this study were to determine the value of microbiological, clinical and histological parameters of Candida albicans colonisation of the dorsal tongue surface as indicators of disease severity, and to evaluate therapeutic response to different formulations of nystatin. Materials and methods: We used 84 males, 2-month-old Sprague–Dawley sialoadenectomized rats. Different formulations of nystatin were used to evaluate the therapeutic response. The animals were randomized to 2 groups with each of 42 animals and received the experimental treatments from day 17–22. Results: 100% of the rats showed evidence of infection. At 5 and 10 days of starting treatment with nystatin + chitosan, and at 10 days of starting nystatin + orabase, the number of animals with positive dorsal tongue culture decreased significantly (p < 0.05), acting the Nystatin + chitosan more rapidly against Candida. In the control group, the percentage of normal papillae on day 22 and 27 was 83.33% (SD = 1.50) and 79.08% (SD = 2.30), respectively. Significant differences were observed in the mean O'Grady score at 5 and 10 days (p < 0.0001). Conclusion: The model has been shown to be effective in inducing infection, and that the combination of nystatin and chitosan yielded the best therapeutic outcomes at both 5 and 10 days after infection. |
| first_indexed | 2024-12-14T03:53:39Z |
| format | Article |
| id | doaj.art-21b2d57b4ecb41c98b5cda1fa63e955f |
| institution | Directory Open Access Journal |
| issn | 1991-7902 |
| language | English |
| last_indexed | 2024-12-14T03:53:39Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dental Sciences |
| spelling | doaj.art-21b2d57b4ecb41c98b5cda1fa63e955f2022-12-21T23:18:08ZengElsevierJournal of Dental Sciences1991-79022021-01-01161123130Efficacy of different formulations of nystatin in an experimental model of oral candidiasis in sialoadenectomized ratsMario Pérez-Sayáns0Renee Beiro-Fuentes1Eva M. Otero-Rey2Cintia Micaela Chamorro-Petronacci3Pilar Gándara-Vila4Jose Manuel Somoza-Martín5Abel García-García6Andrés Blanco-Carrión7Oral Medicine, Oral Surgery and Implantology Unit (MedOralRes Group), Faculty of Medicine and Dentistry University of Santiago de Compostela, Spain; Foundation of Santiago de Compostela Health Research Institute (IDIS), Santiago de Compostela, SpainOral Medicine, Oral Surgery and Implantology Unit (MedOralRes Group), Faculty of Medicine and Dentistry University of Santiago de Compostela, SpainOral Medicine, Oral Surgery and Implantology Unit (MedOralRes Group), Faculty of Medicine and Dentistry University of Santiago de Compostela, SpainOral Medicine, Oral Surgery and Implantology Unit (MedOralRes Group), Faculty of Medicine and Dentistry University of Santiago de Compostela, Spain; Foundation of Santiago de Compostela Health Research Institute (IDIS), Santiago de Compostela, Spain; Corresponding author. Oral Medicine Department, Santiago de Compostela University, Spain.Oral Medicine, Oral Surgery and Implantology Unit (MedOralRes Group), Faculty of Medicine and Dentistry University of Santiago de Compostela, Spain; Foundation of Santiago de Compostela Health Research Institute (IDIS), Santiago de Compostela, SpainOral Medicine, Oral Surgery and Implantology Unit (MedOralRes Group), Faculty of Medicine and Dentistry University of Santiago de Compostela, SpainOral Medicine, Oral Surgery and Implantology Unit (MedOralRes Group), Faculty of Medicine and Dentistry University of Santiago de Compostela, Spain; Foundation of Santiago de Compostela Health Research Institute (IDIS), Santiago de Compostela, SpainOral Medicine, Oral Surgery and Implantology Unit (MedOralRes Group), Faculty of Medicine and Dentistry University of Santiago de Compostela, SpainBackground/purpose: Oral candidiasis is the most common fungal infection of the oral cavity and has become a focus of attention in recent years because of its association with highly topical immunosuppressive conditions. The aims of this study were to determine the value of microbiological, clinical and histological parameters of Candida albicans colonisation of the dorsal tongue surface as indicators of disease severity, and to evaluate therapeutic response to different formulations of nystatin. Materials and methods: We used 84 males, 2-month-old Sprague–Dawley sialoadenectomized rats. Different formulations of nystatin were used to evaluate the therapeutic response. The animals were randomized to 2 groups with each of 42 animals and received the experimental treatments from day 17–22. Results: 100% of the rats showed evidence of infection. At 5 and 10 days of starting treatment with nystatin + chitosan, and at 10 days of starting nystatin + orabase, the number of animals with positive dorsal tongue culture decreased significantly (p < 0.05), acting the Nystatin + chitosan more rapidly against Candida. In the control group, the percentage of normal papillae on day 22 and 27 was 83.33% (SD = 1.50) and 79.08% (SD = 2.30), respectively. Significant differences were observed in the mean O'Grady score at 5 and 10 days (p < 0.0001). Conclusion: The model has been shown to be effective in inducing infection, and that the combination of nystatin and chitosan yielded the best therapeutic outcomes at both 5 and 10 days after infection.http://www.sciencedirect.com/science/article/pii/S1991790220301513Experimental modelsSalivary glandsNystatineC-albicansOral candidiasis |
| spellingShingle | Mario Pérez-Sayáns Renee Beiro-Fuentes Eva M. Otero-Rey Cintia Micaela Chamorro-Petronacci Pilar Gándara-Vila Jose Manuel Somoza-Martín Abel García-García Andrés Blanco-Carrión Efficacy of different formulations of nystatin in an experimental model of oral candidiasis in sialoadenectomized rats Journal of Dental Sciences Experimental models Salivary glands Nystatine C-albicans Oral candidiasis |
| title | Efficacy of different formulations of nystatin in an experimental model of oral candidiasis in sialoadenectomized rats |
| title_full | Efficacy of different formulations of nystatin in an experimental model of oral candidiasis in sialoadenectomized rats |
| title_fullStr | Efficacy of different formulations of nystatin in an experimental model of oral candidiasis in sialoadenectomized rats |
| title_full_unstemmed | Efficacy of different formulations of nystatin in an experimental model of oral candidiasis in sialoadenectomized rats |
| title_short | Efficacy of different formulations of nystatin in an experimental model of oral candidiasis in sialoadenectomized rats |
| title_sort | efficacy of different formulations of nystatin in an experimental model of oral candidiasis in sialoadenectomized rats |
| topic | Experimental models Salivary glands Nystatine C-albicans Oral candidiasis |
| url | http://www.sciencedirect.com/science/article/pii/S1991790220301513 |
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