Forskolin-Loaded Halloysite Nanotubes as Osteoconductive Additive for the Biopolymer Tissue Engineering Scaffolds
Here we report the use of forskolin-modified halloysite nanotubes (HNTs) as a dopant for biopolymer porous hydrogel scaffolds to impart osteoinductive properties. Forskolin is a labdane diterpenoid isolated from the Indian Coleus plant. This small molecule is widely used as a supplement in molecular...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/13/22/3949 |
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| author | Ekaterina Naumenko Ivan Guryanov Elena Zakirova Rawil Fakhrullin |
| author_facet | Ekaterina Naumenko Ivan Guryanov Elena Zakirova Rawil Fakhrullin |
| author_sort | Ekaterina Naumenko |
| collection | DOAJ |
| description | Here we report the use of forskolin-modified halloysite nanotubes (HNTs) as a dopant for biopolymer porous hydrogel scaffolds to impart osteoinductive properties. Forskolin is a labdane diterpenoid isolated from the Indian Coleus plant. This small molecule is widely used as a supplement in molecular biology for cell differentiation. It has been reported in some earlier publications that forskolin can activate osteodifferentiation process by cyclic adenosine monophosphate (c-AMP) signalling activation in stem cells. In presented study it was demonstrated that forskolin release from halloysite-doped scaffolds induced the osteodifferentiation of equine mesenchymal stem cells (MSCs) in vitro without addition of any specific growth factors. The reinforcement of mechanical properties of cells and intercellular space during the osteodifferentiation was demonstrated using atomic force microscopy (AFM). These clay-doped scaffolds may find applications to accelerate the regeneration of horse bone defects by inducing the processes of osteodifferentiation of endogenous MSCs. |
| first_indexed | 2024-03-10T05:06:44Z |
| format | Article |
| id | doaj.art-21b49af209ee4d3fbd7068edbf186bed |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T05:06:44Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-21b49af209ee4d3fbd7068edbf186bed2023-11-23T01:09:18ZengMDPI AGPolymers2073-43602021-11-011322394910.3390/polym13223949Forskolin-Loaded Halloysite Nanotubes as Osteoconductive Additive for the Biopolymer Tissue Engineering ScaffoldsEkaterina Naumenko0Ivan Guryanov1Elena Zakirova2Rawil Fakhrullin3Institute of Fundamental Medicine and Biology, Kazan Federal University, Kreml Uramı 18, Kazan 420008, Republic of Tatarstan, Russian FederationInstitute of Fundamental Medicine and Biology, Kazan Federal University, Kreml Uramı 18, Kazan 420008, Republic of Tatarstan, Russian FederationInstitute of Fundamental Medicine and Biology, Kazan Federal University, Kreml Uramı 18, Kazan 420008, Republic of Tatarstan, Russian FederationInstitute of Fundamental Medicine and Biology, Kazan Federal University, Kreml Uramı 18, Kazan 420008, Republic of Tatarstan, Russian FederationHere we report the use of forskolin-modified halloysite nanotubes (HNTs) as a dopant for biopolymer porous hydrogel scaffolds to impart osteoinductive properties. Forskolin is a labdane diterpenoid isolated from the Indian Coleus plant. This small molecule is widely used as a supplement in molecular biology for cell differentiation. It has been reported in some earlier publications that forskolin can activate osteodifferentiation process by cyclic adenosine monophosphate (c-AMP) signalling activation in stem cells. In presented study it was demonstrated that forskolin release from halloysite-doped scaffolds induced the osteodifferentiation of equine mesenchymal stem cells (MSCs) in vitro without addition of any specific growth factors. The reinforcement of mechanical properties of cells and intercellular space during the osteodifferentiation was demonstrated using atomic force microscopy (AFM). These clay-doped scaffolds may find applications to accelerate the regeneration of horse bone defects by inducing the processes of osteodifferentiation of endogenous MSCs.https://www.mdpi.com/2073-4360/13/22/3949forskolinbiopolymerstissue engineering scaffoldshalloysite nanotubesmesenchymal stem cells |
| spellingShingle | Ekaterina Naumenko Ivan Guryanov Elena Zakirova Rawil Fakhrullin Forskolin-Loaded Halloysite Nanotubes as Osteoconductive Additive for the Biopolymer Tissue Engineering Scaffolds Polymers forskolin biopolymers tissue engineering scaffolds halloysite nanotubes mesenchymal stem cells |
| title | Forskolin-Loaded Halloysite Nanotubes as Osteoconductive Additive for the Biopolymer Tissue Engineering Scaffolds |
| title_full | Forskolin-Loaded Halloysite Nanotubes as Osteoconductive Additive for the Biopolymer Tissue Engineering Scaffolds |
| title_fullStr | Forskolin-Loaded Halloysite Nanotubes as Osteoconductive Additive for the Biopolymer Tissue Engineering Scaffolds |
| title_full_unstemmed | Forskolin-Loaded Halloysite Nanotubes as Osteoconductive Additive for the Biopolymer Tissue Engineering Scaffolds |
| title_short | Forskolin-Loaded Halloysite Nanotubes as Osteoconductive Additive for the Biopolymer Tissue Engineering Scaffolds |
| title_sort | forskolin loaded halloysite nanotubes as osteoconductive additive for the biopolymer tissue engineering scaffolds |
| topic | forskolin biopolymers tissue engineering scaffolds halloysite nanotubes mesenchymal stem cells |
| url | https://www.mdpi.com/2073-4360/13/22/3949 |
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