Development of an Inverted Epifluorescence Microscope for Long-Term Monitoring of Bacteria in Multiplexed Microfluidic Devices
Developing more efficient methods for antibiotic susceptibility testing is a pressing issue in novel drug development as bacterial resistance to antibiotics becomes increasingly common. Microfluidic devices have been demonstrated to be powerful platforms that allow researchers to perform multiplexed...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/20/15/4140 |
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| author | Amaro Torres-Simón María Henar Marino Clara Gómez-Cruz Marina Cañadas Miguel Marco Jorge Ripoll Juan José Vaquero Arrate Muñoz-Barrutia |
| author_facet | Amaro Torres-Simón María Henar Marino Clara Gómez-Cruz Marina Cañadas Miguel Marco Jorge Ripoll Juan José Vaquero Arrate Muñoz-Barrutia |
| author_sort | Amaro Torres-Simón |
| collection | DOAJ |
| description | Developing more efficient methods for antibiotic susceptibility testing is a pressing issue in novel drug development as bacterial resistance to antibiotics becomes increasingly common. Microfluidic devices have been demonstrated to be powerful platforms that allow researchers to perform multiplexed antibiotic testing. However, the level of multiplexing within microdevices is limited, evidencing the need of creating simple, low-cost and high-resolution imaging systems that can be integrated in antibiotic development pipelines. This paper describes the design and development of an epifluorescence inverted microscope that enables long-term monitoring of bacteria inside multiplexed microfluidic devices. The goal of this work is to provide a simple microscope powerful enough to allow single-cell analysis of bacteria at a reduced cost. This facilitates increasing the number of microscopes that are simultaneously used for antibiotic testing. We prove that the designed system is able to accurately detect fluorescent beads of 100 nm, demonstrating comparable features to high-end commercial microscopes and effectively achieving the resolution required for single-cell analysis of bacteria. The proposed microscope could thus increase the efficiency in antibiotic testing while reducing cost, size, weight, and power requirements, contributing to the successful development of new antibiotic drugs. |
| first_indexed | 2024-03-10T18:12:45Z |
| format | Article |
| id | doaj.art-21b635d3bd2d41de8b991bfa479b0b85 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T18:12:45Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-21b635d3bd2d41de8b991bfa479b0b852023-11-20T07:56:01ZengMDPI AGSensors1424-82202020-07-012015414010.3390/s20154140Development of an Inverted Epifluorescence Microscope for Long-Term Monitoring of Bacteria in Multiplexed Microfluidic DevicesAmaro Torres-Simón0María Henar Marino1Clara Gómez-Cruz2Marina Cañadas3Miguel Marco4Jorge Ripoll5Juan José Vaquero6Arrate Muñoz-Barrutia7Departamento de Bioingeniería e Ingeniería Aeroespacial, Universidad Carlos III de Madrid, 28911 Leganés, SpainDepartamento de Bioingeniería e Ingeniería Aeroespacial, Universidad Carlos III de Madrid, 28911 Leganés, SpainDepartamento de Bioingeniería e Ingeniería Aeroespacial, Universidad Carlos III de Madrid, 28911 Leganés, SpainDepartamento de Bioingeniería e Ingeniería Aeroespacial, Universidad Carlos III de Madrid, 28911 Leganés, SpainDepartamento de Ingeniería Mecánica, Universidad Carlos III de Madrid, 28911 Leganés, SpainDepartamento de Bioingeniería e Ingeniería Aeroespacial, Universidad Carlos III de Madrid, 28911 Leganés, SpainDepartamento de Bioingeniería e Ingeniería Aeroespacial, Universidad Carlos III de Madrid, 28911 Leganés, SpainDepartamento de Bioingeniería e Ingeniería Aeroespacial, Universidad Carlos III de Madrid, 28911 Leganés, SpainDeveloping more efficient methods for antibiotic susceptibility testing is a pressing issue in novel drug development as bacterial resistance to antibiotics becomes increasingly common. Microfluidic devices have been demonstrated to be powerful platforms that allow researchers to perform multiplexed antibiotic testing. However, the level of multiplexing within microdevices is limited, evidencing the need of creating simple, low-cost and high-resolution imaging systems that can be integrated in antibiotic development pipelines. This paper describes the design and development of an epifluorescence inverted microscope that enables long-term monitoring of bacteria inside multiplexed microfluidic devices. The goal of this work is to provide a simple microscope powerful enough to allow single-cell analysis of bacteria at a reduced cost. This facilitates increasing the number of microscopes that are simultaneously used for antibiotic testing. We prove that the designed system is able to accurately detect fluorescent beads of 100 nm, demonstrating comparable features to high-end commercial microscopes and effectively achieving the resolution required for single-cell analysis of bacteria. The proposed microscope could thus increase the efficiency in antibiotic testing while reducing cost, size, weight, and power requirements, contributing to the successful development of new antibiotic drugs.https://www.mdpi.com/1424-8220/20/15/4140epifluorescence microscopytime-lapse microscopyfluorescence imagingsingle-cell analysismicrofluidicsGFP-expressing bacteria |
| spellingShingle | Amaro Torres-Simón María Henar Marino Clara Gómez-Cruz Marina Cañadas Miguel Marco Jorge Ripoll Juan José Vaquero Arrate Muñoz-Barrutia Development of an Inverted Epifluorescence Microscope for Long-Term Monitoring of Bacteria in Multiplexed Microfluidic Devices Sensors epifluorescence microscopy time-lapse microscopy fluorescence imaging single-cell analysis microfluidics GFP-expressing bacteria |
| title | Development of an Inverted Epifluorescence Microscope for Long-Term Monitoring of Bacteria in Multiplexed Microfluidic Devices |
| title_full | Development of an Inverted Epifluorescence Microscope for Long-Term Monitoring of Bacteria in Multiplexed Microfluidic Devices |
| title_fullStr | Development of an Inverted Epifluorescence Microscope for Long-Term Monitoring of Bacteria in Multiplexed Microfluidic Devices |
| title_full_unstemmed | Development of an Inverted Epifluorescence Microscope for Long-Term Monitoring of Bacteria in Multiplexed Microfluidic Devices |
| title_short | Development of an Inverted Epifluorescence Microscope for Long-Term Monitoring of Bacteria in Multiplexed Microfluidic Devices |
| title_sort | development of an inverted epifluorescence microscope for long term monitoring of bacteria in multiplexed microfluidic devices |
| topic | epifluorescence microscopy time-lapse microscopy fluorescence imaging single-cell analysis microfluidics GFP-expressing bacteria |
| url | https://www.mdpi.com/1424-8220/20/15/4140 |
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