Isolation and identification of some cyanobacteria and their plant growth promoting effect on wheat (Triticum aestivum L.), Ethiopia
Cyanobacteria are gram negative photosynthetic prokaryotic microorganisms otherwise known as Blue Green Algae (BGA). Numerous cyanobacteria were isolated and identified worldwide and most of them are known to possess the ability to discharge plant growth promoting substances as well as fixing atmosp...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolaita Sodo Univeristy
2020-05-01
|
| Series: | Journal of Science and Inclusive Development |
| Subjects: | |
| Online Access: | https://jsid.edu.et/index.php/jsid/article/view/216 |
| _version_ | 1797725859147153408 |
|---|---|
| author | Krishna Moorthy Sivalingam Abera Batiri |
| author_facet | Krishna Moorthy Sivalingam Abera Batiri |
| author_sort | Krishna Moorthy Sivalingam |
| collection | DOAJ |
| description | Cyanobacteria are gram negative photosynthetic prokaryotic microorganisms otherwise known as Blue Green Algae (BGA). Numerous cyanobacteria were isolated and identified worldwide and most of them are known to possess the ability to discharge plant growth promoting substances as well as fixing atmospheric nitrogen. Therefore, the present study mainly focussed on evaluating the plant growth promoting activity of cyanobacterial isolates using wheat as an experimental crop by seed germination and pot experiments. In the present study, five different cyanobacterial species were isolated and identified as Pseudanabaena galeata KA1, Oscillatoria perornata KA2, Phormidium acutum KA3, Rivularia sp. KA4 and Lyngbya sp. KA5 based on the morphometric characters using microscopic investigations. The heterocystous cyanobacterium Rivularia sp. KA4 at 0.3% aqueous concentration showed significantly (p<0.05) highest results in the morphological parameters as well as in the biochemical parameters under seed germinations experiment. The same heterocystous cyanobacterium Rivularia sp. KA4 at 2g dried application significantly (p<0.05) boosted the morphological growth parameters (plant height, number of leaves, leaf length, leaf width, number of roots, root length, shoot fresh and dry weight) and biochemical parameters (chlorophyll a, chlorophyll b, total chlorophyll and carotenoid) of the experimental crop under pot experiment when compared to all other cyanobacterial species, chemical fertilizer treatment, and control. Therefore, the heterocystous cyanobacterial isolate Rivularia sp. KA4 at 3% aqueous extracts can be used to do the pretreatment of wheat seeds and the same heterocystous cyanobacterium Rivularia sp. KA4 can also be used as biofertilizers in both dried as well as liquid form for the cultivation of T. aestivum. |
| first_indexed | 2024-03-12T10:37:17Z |
| format | Article |
| id | doaj.art-21b96a467f9b48d9b934fbeed00472b1 |
| institution | Directory Open Access Journal |
| issn | 2708-3756 |
| language | English |
| last_indexed | 2024-03-12T10:37:17Z |
| publishDate | 2020-05-01 |
| publisher | Wolaita Sodo Univeristy |
| record_format | Article |
| series | Journal of Science and Inclusive Development |
| spelling | doaj.art-21b96a467f9b48d9b934fbeed00472b12023-09-02T08:27:46ZengWolaita Sodo UniveristyJournal of Science and Inclusive Development2708-37562020-05-01173710.20372/jsid/2020-32216Isolation and identification of some cyanobacteria and their plant growth promoting effect on wheat (Triticum aestivum L.), EthiopiaKrishna Moorthy Sivalingam0Abera Batiri1Department of Biology, Wolaita Sodo University, Ethiopia.Department of Biology, Wolaita Sodo University, EthiopiaCyanobacteria are gram negative photosynthetic prokaryotic microorganisms otherwise known as Blue Green Algae (BGA). Numerous cyanobacteria were isolated and identified worldwide and most of them are known to possess the ability to discharge plant growth promoting substances as well as fixing atmospheric nitrogen. Therefore, the present study mainly focussed on evaluating the plant growth promoting activity of cyanobacterial isolates using wheat as an experimental crop by seed germination and pot experiments. In the present study, five different cyanobacterial species were isolated and identified as Pseudanabaena galeata KA1, Oscillatoria perornata KA2, Phormidium acutum KA3, Rivularia sp. KA4 and Lyngbya sp. KA5 based on the morphometric characters using microscopic investigations. The heterocystous cyanobacterium Rivularia sp. KA4 at 0.3% aqueous concentration showed significantly (p<0.05) highest results in the morphological parameters as well as in the biochemical parameters under seed germinations experiment. The same heterocystous cyanobacterium Rivularia sp. KA4 at 2g dried application significantly (p<0.05) boosted the morphological growth parameters (plant height, number of leaves, leaf length, leaf width, number of roots, root length, shoot fresh and dry weight) and biochemical parameters (chlorophyll a, chlorophyll b, total chlorophyll and carotenoid) of the experimental crop under pot experiment when compared to all other cyanobacterial species, chemical fertilizer treatment, and control. Therefore, the heterocystous cyanobacterial isolate Rivularia sp. KA4 at 3% aqueous extracts can be used to do the pretreatment of wheat seeds and the same heterocystous cyanobacterium Rivularia sp. KA4 can also be used as biofertilizers in both dried as well as liquid form for the cultivation of T. aestivum.https://jsid.edu.et/index.php/jsid/article/view/216cyanobacteria, seed germination, pot experiment, wheat, chemical fertilizer |
| spellingShingle | Krishna Moorthy Sivalingam Abera Batiri Isolation and identification of some cyanobacteria and their plant growth promoting effect on wheat (Triticum aestivum L.), Ethiopia Journal of Science and Inclusive Development cyanobacteria, seed germination, pot experiment, wheat, chemical fertilizer |
| title | Isolation and identification of some cyanobacteria and their plant growth promoting effect on wheat (Triticum aestivum L.), Ethiopia |
| title_full | Isolation and identification of some cyanobacteria and their plant growth promoting effect on wheat (Triticum aestivum L.), Ethiopia |
| title_fullStr | Isolation and identification of some cyanobacteria and their plant growth promoting effect on wheat (Triticum aestivum L.), Ethiopia |
| title_full_unstemmed | Isolation and identification of some cyanobacteria and their plant growth promoting effect on wheat (Triticum aestivum L.), Ethiopia |
| title_short | Isolation and identification of some cyanobacteria and their plant growth promoting effect on wheat (Triticum aestivum L.), Ethiopia |
| title_sort | isolation and identification of some cyanobacteria and their plant growth promoting effect on wheat triticum aestivum l ethiopia |
| topic | cyanobacteria, seed germination, pot experiment, wheat, chemical fertilizer |
| url | https://jsid.edu.et/index.php/jsid/article/view/216 |
| work_keys_str_mv | AT krishnamoorthysivalingam isolationandidentificationofsomecyanobacteriaandtheirplantgrowthpromotingeffectonwheattriticumaestivumlethiopia AT aberabatiri isolationandidentificationofsomecyanobacteriaandtheirplantgrowthpromotingeffectonwheattriticumaestivumlethiopia |