Inheritance of Early and Late Ascochyta Blight Resistance in Wide Crosses of Chickpea
Chickpea (<i>Cicer arietinum</i>) is a globally important food legume but its yield is negatively impacted by the fungal pathogen Ascochyta blight (<i>Ascochyta rabiei</i>) causing necrotic lesions leading to plant death. Past studies have found that Ascochyta resistance is p...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
|
| Series: | Genes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4425/14/2/316 |
| _version_ | 1797620790000091136 |
|---|---|
| author | Abdulkarim Lakmes Abdullah Jhar Adrian C. Brennan Abdullah Kahriman |
| author_facet | Abdulkarim Lakmes Abdullah Jhar Adrian C. Brennan Abdullah Kahriman |
| author_sort | Abdulkarim Lakmes |
| collection | DOAJ |
| description | Chickpea (<i>Cicer arietinum</i>) is a globally important food legume but its yield is negatively impacted by the fungal pathogen Ascochyta blight (<i>Ascochyta rabiei</i>) causing necrotic lesions leading to plant death. Past studies have found that Ascochyta resistance is polygenic. It is important to find new resistance genes from the wider genepool of chickpeas. This study reports the inheritance of Ascochyta blight resistance of two wide crosses between the cultivar Gokce and wild chickpea accessions of <i>C. reticulatum</i> and <i>C. echinospermum</i> under field conditions in Southern Turkey. Following inoculation, infection damage was scored weekly for six weeks. The families were genotyped for 60 SNPs mapped to the reference genome for quantitative locus (QTL) mapping of resistance. Family lines showed broad resistance score distributions. A late responding QTL on chromosome 7 was identified in the <i>C. reticulatum</i> family and three early responding QTLs on chromosomes 2, 3, and 6 in the <i>C. echinospermum</i> family. Wild alleles mostly showed reduced disease severity, while heterozygous genotypes were most diseased. Interrogation of 200k bp genomic regions of the reference CDC Frontier genome surrounding QTLs identified nine gene candidates involved in disease resistance and cell wall remodeling. This study identifies new candidate chickpea Ascochyta blight resistance QTLs of breeding potential. |
| first_indexed | 2024-03-11T08:46:37Z |
| format | Article |
| id | doaj.art-21b98d797f6f4ee9848ec079cf169815 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-11T08:46:37Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-21b98d797f6f4ee9848ec079cf1698152023-11-16T20:41:17ZengMDPI AGGenes2073-44252023-01-0114231610.3390/genes14020316Inheritance of Early and Late Ascochyta Blight Resistance in Wide Crosses of ChickpeaAbdulkarim Lakmes0Abdullah Jhar1Adrian C. Brennan2Abdullah Kahriman3Department of Field Crops, Harran University, Sanliurfa 63100, TurkeyDepartment of Field Crops, Harran University, Sanliurfa 63100, TurkeyBiosciences Department, Durham University, Durham DH1 3LE, UKDepartment of Field Crops, Harran University, Sanliurfa 63100, TurkeyChickpea (<i>Cicer arietinum</i>) is a globally important food legume but its yield is negatively impacted by the fungal pathogen Ascochyta blight (<i>Ascochyta rabiei</i>) causing necrotic lesions leading to plant death. Past studies have found that Ascochyta resistance is polygenic. It is important to find new resistance genes from the wider genepool of chickpeas. This study reports the inheritance of Ascochyta blight resistance of two wide crosses between the cultivar Gokce and wild chickpea accessions of <i>C. reticulatum</i> and <i>C. echinospermum</i> under field conditions in Southern Turkey. Following inoculation, infection damage was scored weekly for six weeks. The families were genotyped for 60 SNPs mapped to the reference genome for quantitative locus (QTL) mapping of resistance. Family lines showed broad resistance score distributions. A late responding QTL on chromosome 7 was identified in the <i>C. reticulatum</i> family and three early responding QTLs on chromosomes 2, 3, and 6 in the <i>C. echinospermum</i> family. Wild alleles mostly showed reduced disease severity, while heterozygous genotypes were most diseased. Interrogation of 200k bp genomic regions of the reference CDC Frontier genome surrounding QTLs identified nine gene candidates involved in disease resistance and cell wall remodeling. This study identifies new candidate chickpea Ascochyta blight resistance QTLs of breeding potential.https://www.mdpi.com/2073-4425/14/2/316Ascochyta blight<i>Cicer arietinum</i>wild crop relativespathogen resistancequantitative trait locuspolygenic inheritance |
| spellingShingle | Abdulkarim Lakmes Abdullah Jhar Adrian C. Brennan Abdullah Kahriman Inheritance of Early and Late Ascochyta Blight Resistance in Wide Crosses of Chickpea Genes Ascochyta blight <i>Cicer arietinum</i> wild crop relatives pathogen resistance quantitative trait locus polygenic inheritance |
| title | Inheritance of Early and Late Ascochyta Blight Resistance in Wide Crosses of Chickpea |
| title_full | Inheritance of Early and Late Ascochyta Blight Resistance in Wide Crosses of Chickpea |
| title_fullStr | Inheritance of Early and Late Ascochyta Blight Resistance in Wide Crosses of Chickpea |
| title_full_unstemmed | Inheritance of Early and Late Ascochyta Blight Resistance in Wide Crosses of Chickpea |
| title_short | Inheritance of Early and Late Ascochyta Blight Resistance in Wide Crosses of Chickpea |
| title_sort | inheritance of early and late ascochyta blight resistance in wide crosses of chickpea |
| topic | Ascochyta blight <i>Cicer arietinum</i> wild crop relatives pathogen resistance quantitative trait locus polygenic inheritance |
| url | https://www.mdpi.com/2073-4425/14/2/316 |
| work_keys_str_mv | AT abdulkarimlakmes inheritanceofearlyandlateascochytablightresistanceinwidecrossesofchickpea AT abdullahjhar inheritanceofearlyandlateascochytablightresistanceinwidecrossesofchickpea AT adriancbrennan inheritanceofearlyandlateascochytablightresistanceinwidecrossesofchickpea AT abdullahkahriman inheritanceofearlyandlateascochytablightresistanceinwidecrossesofchickpea |