Influence of Phospholipid and Protein Constituents on Tribological Properties of Artificial Hydrogel Cartilage Material
In this study, the influence of phospholipid and protein constituents on friction and wear behavior of artificial hydrogel cartilage was investigated. A sliding pair of an ellipsoidal specimen of poly (vinyl alcohol) (PVA) hydrogel and a flat specimen of PVA hydrogel was evaluated in simplified reci...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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The Japan Society of Mechanical Engineers
2013-09-01
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| Series: | Journal of Biomechanical Science and Engineering |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jbse/8/3/8_257/_pdf/-char/en |
| _version_ | 1811225604388814848 |
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| author | Seido YARIMITSU Kazuhiro NAKASHIMA Yoshinori SAWAE Teruo MURAKAMI |
| author_facet | Seido YARIMITSU Kazuhiro NAKASHIMA Yoshinori SAWAE Teruo MURAKAMI |
| author_sort | Seido YARIMITSU |
| collection | DOAJ |
| description | In this study, the influence of phospholipid and protein constituents on friction and wear behavior of artificial hydrogel cartilage was investigated. A sliding pair of an ellipsoidal specimen of poly (vinyl alcohol) (PVA) hydrogel and a flat specimen of PVA hydrogel was evaluated in simplified reciprocating friction test. Dipalmitoylphosphatidylcholine (DPPC) was selected as a phospholipid constituent and was dispersed in saline as liposome. Fluorescent-labeled albumin and γ-globulin were used as protein constituents in lubricants at concentration of 0.7 wt%. After reciprocating friction test, the boundary film formed on the surface of PVA hydrogel and the worn surface of PVA hydrogel were observed by using fluorescent microscope and confocal laser scanning microscope, respectively. When only albumin or γ-globulin was added to lubricant, adhesive wear pattern was frequently observed and large breaking-off of surface structure of PVA hydrogel occurred. Lubricants that contain both proteins and 0.01wt% DPPC showed reduction of friction and suppression of large breaking-off of surface structure of PVA hydrogel. Meanwhile, under coexistence of protein and 0.02wt% DPPC, friction increased compared to that for lubricants that contain 0.01wt% DPPC and the adhesive wear patterns became obvious. Therefore, both the concentration and the relative ratio of proteins to phospholipids are important factors to function adequately as excellent boundary lubricant for PVA hydrogel. |
| first_indexed | 2024-04-12T09:10:09Z |
| format | Article |
| id | doaj.art-21bad8afbdaf485485503c45356b5b59 |
| institution | Directory Open Access Journal |
| issn | 1880-9863 |
| language | English |
| last_indexed | 2024-04-12T09:10:09Z |
| publishDate | 2013-09-01 |
| publisher | The Japan Society of Mechanical Engineers |
| record_format | Article |
| series | Journal of Biomechanical Science and Engineering |
| spelling | doaj.art-21bad8afbdaf485485503c45356b5b592022-12-22T03:39:00ZengThe Japan Society of Mechanical EngineersJournal of Biomechanical Science and Engineering1880-98632013-09-018325726710.1299/jbse.8.257jbseInfluence of Phospholipid and Protein Constituents on Tribological Properties of Artificial Hydrogel Cartilage MaterialSeido YARIMITSU0Kazuhiro NAKASHIMA1Yoshinori SAWAE2Teruo MURAKAMI3Research Center for Advanced Biomechanics, Kyushu UniversityResearch Center for Advanced Biomechanics, Kyushu UniversityResearch Center for Advanced Biomechanics, Kyushu UniversityResearch Center for Advanced Biomechanics, Kyushu UniversityIn this study, the influence of phospholipid and protein constituents on friction and wear behavior of artificial hydrogel cartilage was investigated. A sliding pair of an ellipsoidal specimen of poly (vinyl alcohol) (PVA) hydrogel and a flat specimen of PVA hydrogel was evaluated in simplified reciprocating friction test. Dipalmitoylphosphatidylcholine (DPPC) was selected as a phospholipid constituent and was dispersed in saline as liposome. Fluorescent-labeled albumin and γ-globulin were used as protein constituents in lubricants at concentration of 0.7 wt%. After reciprocating friction test, the boundary film formed on the surface of PVA hydrogel and the worn surface of PVA hydrogel were observed by using fluorescent microscope and confocal laser scanning microscope, respectively. When only albumin or γ-globulin was added to lubricant, adhesive wear pattern was frequently observed and large breaking-off of surface structure of PVA hydrogel occurred. Lubricants that contain both proteins and 0.01wt% DPPC showed reduction of friction and suppression of large breaking-off of surface structure of PVA hydrogel. Meanwhile, under coexistence of protein and 0.02wt% DPPC, friction increased compared to that for lubricants that contain 0.01wt% DPPC and the adhesive wear patterns became obvious. Therefore, both the concentration and the relative ratio of proteins to phospholipids are important factors to function adequately as excellent boundary lubricant for PVA hydrogel.https://www.jstage.jst.go.jp/article/jbse/8/3/8_257/_pdf/-char/enartificial hydrogel cartilagephospholipidproteinfrictionwear |
| spellingShingle | Seido YARIMITSU Kazuhiro NAKASHIMA Yoshinori SAWAE Teruo MURAKAMI Influence of Phospholipid and Protein Constituents on Tribological Properties of Artificial Hydrogel Cartilage Material Journal of Biomechanical Science and Engineering artificial hydrogel cartilage phospholipid protein friction wear |
| title | Influence of Phospholipid and Protein Constituents on Tribological Properties of Artificial Hydrogel Cartilage Material |
| title_full | Influence of Phospholipid and Protein Constituents on Tribological Properties of Artificial Hydrogel Cartilage Material |
| title_fullStr | Influence of Phospholipid and Protein Constituents on Tribological Properties of Artificial Hydrogel Cartilage Material |
| title_full_unstemmed | Influence of Phospholipid and Protein Constituents on Tribological Properties of Artificial Hydrogel Cartilage Material |
| title_short | Influence of Phospholipid and Protein Constituents on Tribological Properties of Artificial Hydrogel Cartilage Material |
| title_sort | influence of phospholipid and protein constituents on tribological properties of artificial hydrogel cartilage material |
| topic | artificial hydrogel cartilage phospholipid protein friction wear |
| url | https://www.jstage.jst.go.jp/article/jbse/8/3/8_257/_pdf/-char/en |
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