Sensitivity of a Vacuum Aspiratory Culture Technique for Diagnosis of Localized Cutaneous Leishmaniasis in an Endemic Area of Leishmania (Viannia) braziliensis Transmission
Sixty eight patients with localized cutaneous leishmaniasis from an area with Leishmania (Viannia) braziliensis transmission had cultures performed with a modified Marzochi´s vacuum aspiratory puncture technique to establish sensitivity and contamination rate with this new method. Overall sensitivit...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
1999-01-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761999000400014 |
| _version_ | 1797758423531520000 |
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| author | Romero Gustavo Adolfo Sierra Sampaio Raimunda Nonata Ribeiro Macêdo Vanize de Oliveira Marsden Philip Davis |
| author_facet | Romero Gustavo Adolfo Sierra Sampaio Raimunda Nonata Ribeiro Macêdo Vanize de Oliveira Marsden Philip Davis |
| author_sort | Romero Gustavo Adolfo Sierra |
| collection | DOAJ |
| description | Sixty eight patients with localized cutaneous leishmaniasis from an area with Leishmania (Viannia) braziliensis transmission had cultures performed with a modified Marzochi´s vacuum aspiratory puncture technique to establish sensitivity and contamination rate with this new method. Overall sensitivity of three aspirates was 47.1%; (CI95% 39.4; 59.4) significantly greater than the sensitivity of a single one aspirate. Fungal contamination was observed in 6/204 (2.9%) inoculated culture tubes. We recommend that this useful technique should be adopted as routine for primary isolation of L. (V.) braziliensis from localized cutaneous ulcers. |
| first_indexed | 2024-03-12T18:29:46Z |
| format | Article |
| id | doaj.art-21bc7ec8542a4812a76317a3f4360873 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:29:46Z |
| publishDate | 1999-01-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-21bc7ec8542a4812a76317a3f43608732023-08-02T08:19:32ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601999-01-01944505508Sensitivity of a Vacuum Aspiratory Culture Technique for Diagnosis of Localized Cutaneous Leishmaniasis in an Endemic Area of Leishmania (Viannia) braziliensis TransmissionRomero Gustavo Adolfo SierraSampaio Raimunda Nonata RibeiroMacêdo Vanize de OliveiraMarsden Philip DavisSixty eight patients with localized cutaneous leishmaniasis from an area with Leishmania (Viannia) braziliensis transmission had cultures performed with a modified Marzochi´s vacuum aspiratory puncture technique to establish sensitivity and contamination rate with this new method. Overall sensitivity of three aspirates was 47.1%; (CI95% 39.4; 59.4) significantly greater than the sensitivity of a single one aspirate. Fungal contamination was observed in 6/204 (2.9%) inoculated culture tubes. We recommend that this useful technique should be adopted as routine for primary isolation of L. (V.) braziliensis from localized cutaneous ulcers.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761999000400014Leishmania (Viannia) braziliensisculture sensitivityfungal contaminationvacuum aspiratory technique |
| spellingShingle | Romero Gustavo Adolfo Sierra Sampaio Raimunda Nonata Ribeiro Macêdo Vanize de Oliveira Marsden Philip Davis Sensitivity of a Vacuum Aspiratory Culture Technique for Diagnosis of Localized Cutaneous Leishmaniasis in an Endemic Area of Leishmania (Viannia) braziliensis Transmission Memorias do Instituto Oswaldo Cruz Leishmania (Viannia) braziliensis culture sensitivity fungal contamination vacuum aspiratory technique |
| title | Sensitivity of a Vacuum Aspiratory Culture Technique for Diagnosis of Localized Cutaneous Leishmaniasis in an Endemic Area of Leishmania (Viannia) braziliensis Transmission |
| title_full | Sensitivity of a Vacuum Aspiratory Culture Technique for Diagnosis of Localized Cutaneous Leishmaniasis in an Endemic Area of Leishmania (Viannia) braziliensis Transmission |
| title_fullStr | Sensitivity of a Vacuum Aspiratory Culture Technique for Diagnosis of Localized Cutaneous Leishmaniasis in an Endemic Area of Leishmania (Viannia) braziliensis Transmission |
| title_full_unstemmed | Sensitivity of a Vacuum Aspiratory Culture Technique for Diagnosis of Localized Cutaneous Leishmaniasis in an Endemic Area of Leishmania (Viannia) braziliensis Transmission |
| title_short | Sensitivity of a Vacuum Aspiratory Culture Technique for Diagnosis of Localized Cutaneous Leishmaniasis in an Endemic Area of Leishmania (Viannia) braziliensis Transmission |
| title_sort | sensitivity of a vacuum aspiratory culture technique for diagnosis of localized cutaneous leishmaniasis in an endemic area of leishmania viannia braziliensis transmission |
| topic | Leishmania (Viannia) braziliensis culture sensitivity fungal contamination vacuum aspiratory technique |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761999000400014 |
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