Research of Genetic Polymorphism Species Linumu sitatissimum L. on a Basis a RAPD-Method

The goal of this investigation was the interpretation of genetic polymorphism in flax using the random amplified polymorphic DNA (RAPD-PCR) technique in relation to genealogical information and eco geographic origin of the accessions. Protein markers have been successfully applied for identification...

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Main Authors: T. A. Rozhmina, Y.-B. Fu, A. Diederichsen, K. W. Richards, M. Pavelek, Miroslava Vrbova
Format: Article
Language:English
Published: Taylor & Francis Group 2018-03-01
Series:Journal of Natural Fibers
Subjects:
Online Access:http://dx.doi.org/10.1080/15440478.2016.1193083
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author T. A. Rozhmina
Y.-B. Fu
A. Diederichsen
K. W. Richards
M. Pavelek
Miroslava Vrbova
author_facet T. A. Rozhmina
Y.-B. Fu
A. Diederichsen
K. W. Richards
M. Pavelek
Miroslava Vrbova
author_sort T. A. Rozhmina
collection DOAJ
description The goal of this investigation was the interpretation of genetic polymorphism in flax using the random amplified polymorphic DNA (RAPD-PCR) technique in relation to genealogical information and eco geographic origin of the accessions. Protein markers have been successfully applied for identification of ecotypes of cultivated plants and for cultivar identification and registration. However, for intraspecific differentiation in flax effective protein markers have not been found. The DNA markers developed during the recent decades proved to be more efficient in detecting polymorphism in flax. The plant material were 287 accessions from the flax collection at the All-Russian Flax Research Institute (VNIIL) belonging to different botanical and eco-geographical groups based on the classification of the species Linumu sitatissimum L. On the basis of a molecular estimation the gene pool offlax it can be assumed that the fiber flax from northern continental Russia is not exclusively of Indo-afghan origin as suggested by Sinskaja (1959), but also has genetic roots in flax from Kolchidian. Essential genetic similarity between cultivar of fiber flax from Russia and other the European countries is established. Results of a generality of an origin of fiber flax cultivar from Russia are confirmed also with the analysis of their genealogy. Essential genetic polymorphism of linseed flax is shown. The distinctness of linseed and fiber flax in their genetic constitution as revealed by the RAPD analysis is of strategic importance in preservation of the genetic diversity and for efficient use of the flax gene pool in breeding.
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spelling doaj.art-21bd105e4859499893a4bbeaaf9cbb842023-09-22T14:19:05ZengTaylor & Francis GroupJournal of Natural Fibers1544-04781544-046X2018-03-0115215516110.1080/15440478.2016.11930831193083Research of Genetic Polymorphism Species Linumu sitatissimum L. on a Basis a RAPD-MethodT. A. Rozhmina0Y.-B. Fu1A. Diederichsen2K. W. Richards3M. Pavelek4Miroslava Vrbova5All-Russian Flax Research Institute (VNIIL)Plant Gene Resources of Canada (PGRC), Saskatoon Research CenterPlant Gene Resources of Canada (PGRC), Saskatoon Research CenterPlant Gene Resources of Canada (PGRC), Saskatoon Research CenterAgritec Plant Research LtdAgritec Plant Research LtdThe goal of this investigation was the interpretation of genetic polymorphism in flax using the random amplified polymorphic DNA (RAPD-PCR) technique in relation to genealogical information and eco geographic origin of the accessions. Protein markers have been successfully applied for identification of ecotypes of cultivated plants and for cultivar identification and registration. However, for intraspecific differentiation in flax effective protein markers have not been found. The DNA markers developed during the recent decades proved to be more efficient in detecting polymorphism in flax. The plant material were 287 accessions from the flax collection at the All-Russian Flax Research Institute (VNIIL) belonging to different botanical and eco-geographical groups based on the classification of the species Linumu sitatissimum L. On the basis of a molecular estimation the gene pool offlax it can be assumed that the fiber flax from northern continental Russia is not exclusively of Indo-afghan origin as suggested by Sinskaja (1959), but also has genetic roots in flax from Kolchidian. Essential genetic similarity between cultivar of fiber flax from Russia and other the European countries is established. Results of a generality of an origin of fiber flax cultivar from Russia are confirmed also with the analysis of their genealogy. Essential genetic polymorphism of linseed flax is shown. The distinctness of linseed and fiber flax in their genetic constitution as revealed by the RAPD analysis is of strategic importance in preservation of the genetic diversity and for efficient use of the flax gene pool in breeding.http://dx.doi.org/10.1080/15440478.2016.1193083flaxlinum usitatissimumrapdgenetic polymorphism
spellingShingle T. A. Rozhmina
Y.-B. Fu
A. Diederichsen
K. W. Richards
M. Pavelek
Miroslava Vrbova
Research of Genetic Polymorphism Species Linumu sitatissimum L. on a Basis a RAPD-Method
Journal of Natural Fibers
flax
linum usitatissimum
rapd
genetic polymorphism
title Research of Genetic Polymorphism Species Linumu sitatissimum L. on a Basis a RAPD-Method
title_full Research of Genetic Polymorphism Species Linumu sitatissimum L. on a Basis a RAPD-Method
title_fullStr Research of Genetic Polymorphism Species Linumu sitatissimum L. on a Basis a RAPD-Method
title_full_unstemmed Research of Genetic Polymorphism Species Linumu sitatissimum L. on a Basis a RAPD-Method
title_short Research of Genetic Polymorphism Species Linumu sitatissimum L. on a Basis a RAPD-Method
title_sort research of genetic polymorphism species linumu sitatissimum l on a basis a rapd method
topic flax
linum usitatissimum
rapd
genetic polymorphism
url http://dx.doi.org/10.1080/15440478.2016.1193083
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