Label-free quantitative analysis of lipid metabolism in living Caenorhabditis elegans[S]
The ubiquity of lipids in biological structures and functions suggests that lipid metabolisms are highly regulated. However, current invasive techniques for lipid studies prevent characterization of the dynamic interactions between various lipid metabolism pathways. Here, we describe a noninvasive a...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2010-03-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520305253 |
| _version_ | 1831666079066226688 |
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| author | Thuc T. Le Holli M. Duren Mikhail N. Slipchenko Chang-Deng Hu Ji-Xin Cheng |
| author_facet | Thuc T. Le Holli M. Duren Mikhail N. Slipchenko Chang-Deng Hu Ji-Xin Cheng |
| author_sort | Thuc T. Le |
| collection | DOAJ |
| description | The ubiquity of lipids in biological structures and functions suggests that lipid metabolisms are highly regulated. However, current invasive techniques for lipid studies prevent characterization of the dynamic interactions between various lipid metabolism pathways. Here, we describe a noninvasive approach to study lipid metabolisms using a multifunctional coherent anti-Stokes Raman scattering (CARS) microscope. Using living Caenorhabditis elegans as a model organism, we report label-free visualization of coexisting neutral and autofluorescent lipid species. We find that the relative expression level of neutral and autofluorescent lipid species can be used to assay the genotype-phenotype relationship of mutant C. elegans with deletions in the genes encoding lipid synthesis transcription factors, LDL receptors, transforming growth factor β receptors, lipid desaturation enzymes, and antioxidant enzymes. Furthermore, by coupling CARS with fingerprint confocal Raman analysis, we analyze the unsaturation level of lipids in wild-type and mutant C. elegans. Our study shows that complex genotype-phenotype relationships between lipid storage, peroxidation, and desaturation can be rapidly and quantitatively analyzed in a single living C. elegans. |
| first_indexed | 2024-12-19T19:46:05Z |
| format | Article |
| id | doaj.art-21bef8dbbd1f45b0885c87b12419c85e |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-19T19:46:05Z |
| publishDate | 2010-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-21bef8dbbd1f45b0885c87b12419c85e2022-12-21T20:08:07ZengElsevierJournal of Lipid Research0022-22752010-03-01513672677Label-free quantitative analysis of lipid metabolism in living Caenorhabditis elegans[S]Thuc T. Le0Holli M. Duren1Mikhail N. Slipchenko2Chang-Deng Hu3Ji-Xin Cheng4Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907To whom correspondence should be addressed; Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907; Purdue Cancer Center, Purdue University, West Lafayette, IN 47907To whom correspondence should be addressed; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907; Purdue Cancer Center, Purdue University, West Lafayette, IN 47907; Department of Chemistry, Purdue University, West Lafayette, IN 47907The ubiquity of lipids in biological structures and functions suggests that lipid metabolisms are highly regulated. However, current invasive techniques for lipid studies prevent characterization of the dynamic interactions between various lipid metabolism pathways. Here, we describe a noninvasive approach to study lipid metabolisms using a multifunctional coherent anti-Stokes Raman scattering (CARS) microscope. Using living Caenorhabditis elegans as a model organism, we report label-free visualization of coexisting neutral and autofluorescent lipid species. We find that the relative expression level of neutral and autofluorescent lipid species can be used to assay the genotype-phenotype relationship of mutant C. elegans with deletions in the genes encoding lipid synthesis transcription factors, LDL receptors, transforming growth factor β receptors, lipid desaturation enzymes, and antioxidant enzymes. Furthermore, by coupling CARS with fingerprint confocal Raman analysis, we analyze the unsaturation level of lipids in wild-type and mutant C. elegans. Our study shows that complex genotype-phenotype relationships between lipid storage, peroxidation, and desaturation can be rapidly and quantitatively analyzed in a single living C. elegans.http://www.sciencedirect.com/science/article/pii/S0022227520305253cholesterol synthesiscoherent anti-Stokes Raman scatteringlipid desaturationlabel-free imaginglipid storageperoxidation |
| spellingShingle | Thuc T. Le Holli M. Duren Mikhail N. Slipchenko Chang-Deng Hu Ji-Xin Cheng Label-free quantitative analysis of lipid metabolism in living Caenorhabditis elegans[S] Journal of Lipid Research cholesterol synthesis coherent anti-Stokes Raman scattering lipid desaturation label-free imaging lipid storage peroxidation |
| title | Label-free quantitative analysis of lipid metabolism in living Caenorhabditis elegans[S] |
| title_full | Label-free quantitative analysis of lipid metabolism in living Caenorhabditis elegans[S] |
| title_fullStr | Label-free quantitative analysis of lipid metabolism in living Caenorhabditis elegans[S] |
| title_full_unstemmed | Label-free quantitative analysis of lipid metabolism in living Caenorhabditis elegans[S] |
| title_short | Label-free quantitative analysis of lipid metabolism in living Caenorhabditis elegans[S] |
| title_sort | label free quantitative analysis of lipid metabolism in living caenorhabditis elegans s |
| topic | cholesterol synthesis coherent anti-Stokes Raman scattering lipid desaturation label-free imaging lipid storage peroxidation |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520305253 |
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