Comparison on the sensitivity of laboratory diagnosis technology in the diagnosis of fungal keratitis
AIM: To analyze the correlation and clinical significance of fungal smear, fungal culture and pathological examination in the diagnosis of fungalkeratitis. <p>METHODS:One hundred and ten cases(110 eyes)with fungal keratitis from January 2012 to December 2014 were collected. The results of fung...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2015-08-01
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| Series: | Guoji Yanke Zazhi |
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| Online Access: | http://ies.ijo.cn/cn_publish/2015/8/201508005.pdf |
| _version_ | 1818042551825858560 |
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| author | Peng-Fei Chen Xiao-Yi Qin Li-Ping Mao You-Pei Wang Da-Xuan Wang Mei-Qin Zheng |
| author_facet | Peng-Fei Chen Xiao-Yi Qin Li-Ping Mao You-Pei Wang Da-Xuan Wang Mei-Qin Zheng |
| author_sort | Peng-Fei Chen |
| collection | DOAJ |
| description | AIM: To analyze the correlation and clinical significance of fungal smear, fungal culture and pathological examination in the diagnosis of fungalkeratitis. <p>METHODS:One hundred and ten cases(110 eyes)with fungal keratitis from January 2012 to December 2014 were collected. The results of fungal smear, fungal culture and pathological examination results were analyzed retrospectively. Fungal smear was detected by 10% KOH wet microscopy and gram staining microscopy. Fungal culture was used potato dextrose agar(PDA)medium. The specimens of pathological examination were from corneal transplantation surgery. paraffin section, HE and hexamine silver and PAS staining was used in the pathological examination. <p>RESULTS:Of the 110 cases of fungal keratitis, fungal smear positive were observed in 50 cases(45.5%), fungal culture positive were observed in 55 cases(50.0%); pathological examination positive were observed in 88 cases(80.0%). Fifty cases were both fungal smear and pathological examination positive and 22 cases were both fungal smear and pathological examination negative. The coincidence rate of fungal smear and pathologic examination was 65.5%. Fifty-five cases were both fungal culture and pathological examination positive and 22 cases were both fungal culture and pathological examination negative. The coincidence rate of fungal culture and pathologic examination was 70.0%. In the 60 cases of fungal smear negative results, 38 cases(63.3%)were confirmed positive through pathological examination. In the 55 cases of fungus culture negative results, 33 cases(60.0%)were confirmed positive by pathological examination. <p>CONCLUSION:The accuracy of pathological examination is the highest. The combined application of fungal smear, fungal culture and pathological examination can improve the diagnostic accuracy of fungal keratitis. |
| first_indexed | 2024-12-10T08:48:07Z |
| format | Article |
| id | doaj.art-21bef9337d974c41b3330ee095a21bdc |
| institution | Directory Open Access Journal |
| issn | 1672-5123 1672-5123 |
| language | English |
| last_indexed | 2024-12-10T08:48:07Z |
| publishDate | 2015-08-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | Guoji Yanke Zazhi |
| spelling | doaj.art-21bef9337d974c41b3330ee095a21bdc2022-12-22T01:55:40ZengPress of International Journal of Ophthalmology (IJO PRESS)Guoji Yanke Zazhi1672-51231672-51232015-08-011581322132610.3980/j.issn.1672-5123.2015.8.05Comparison on the sensitivity of laboratory diagnosis technology in the diagnosis of fungal keratitisPeng-Fei Chen0Xiao-Yi Qin1Li-Ping Mao2You-Pei Wang3Da-Xuan Wang4Mei-Qin Zheng5Clinical Examination Center, Eye Hospital, Wenzhou Medical University, Eye Hospital of Zhejiang Province, Wenzhou 325027, Zhejiang Province, ChinaClinical Examination Center, Eye Hospital, Wenzhou Medical University, Eye Hospital of Zhejiang Province, Wenzhou 325027, Zhejiang Province, ChinaClinical Examination Center, Eye Hospital, Wenzhou Medical University, Eye Hospital of Zhejiang Province, Wenzhou 325027, Zhejiang Province, ChinaClinical Examination Center, Eye Hospital, Wenzhou Medical University, Eye Hospital of Zhejiang Province, Wenzhou 325027, Zhejiang Province, ChinaClinical Examination Center, Eye Hospital, Wenzhou Medical University, Eye Hospital of Zhejiang Province, Wenzhou 325027, Zhejiang Province, ChinaClinical Examination Center, Eye Hospital, Wenzhou Medical University, Eye Hospital of Zhejiang Province, Wenzhou 325027, Zhejiang Province, ChinaAIM: To analyze the correlation and clinical significance of fungal smear, fungal culture and pathological examination in the diagnosis of fungalkeratitis. <p>METHODS:One hundred and ten cases(110 eyes)with fungal keratitis from January 2012 to December 2014 were collected. The results of fungal smear, fungal culture and pathological examination results were analyzed retrospectively. Fungal smear was detected by 10% KOH wet microscopy and gram staining microscopy. Fungal culture was used potato dextrose agar(PDA)medium. The specimens of pathological examination were from corneal transplantation surgery. paraffin section, HE and hexamine silver and PAS staining was used in the pathological examination. <p>RESULTS:Of the 110 cases of fungal keratitis, fungal smear positive were observed in 50 cases(45.5%), fungal culture positive were observed in 55 cases(50.0%); pathological examination positive were observed in 88 cases(80.0%). Fifty cases were both fungal smear and pathological examination positive and 22 cases were both fungal smear and pathological examination negative. The coincidence rate of fungal smear and pathologic examination was 65.5%. Fifty-five cases were both fungal culture and pathological examination positive and 22 cases were both fungal culture and pathological examination negative. The coincidence rate of fungal culture and pathologic examination was 70.0%. In the 60 cases of fungal smear negative results, 38 cases(63.3%)were confirmed positive through pathological examination. In the 55 cases of fungus culture negative results, 33 cases(60.0%)were confirmed positive by pathological examination. <p>CONCLUSION:The accuracy of pathological examination is the highest. The combined application of fungal smear, fungal culture and pathological examination can improve the diagnostic accuracy of fungal keratitis.http://ies.ijo.cn/cn_publish/2015/8/201508005.pdfkeratitisfungalfungal smearfungal culturepathological examination |
| spellingShingle | Peng-Fei Chen Xiao-Yi Qin Li-Ping Mao You-Pei Wang Da-Xuan Wang Mei-Qin Zheng Comparison on the sensitivity of laboratory diagnosis technology in the diagnosis of fungal keratitis Guoji Yanke Zazhi keratitis fungal fungal smear fungal culture pathological examination |
| title | Comparison on the sensitivity of laboratory diagnosis technology in the diagnosis of fungal keratitis |
| title_full | Comparison on the sensitivity of laboratory diagnosis technology in the diagnosis of fungal keratitis |
| title_fullStr | Comparison on the sensitivity of laboratory diagnosis technology in the diagnosis of fungal keratitis |
| title_full_unstemmed | Comparison on the sensitivity of laboratory diagnosis technology in the diagnosis of fungal keratitis |
| title_short | Comparison on the sensitivity of laboratory diagnosis technology in the diagnosis of fungal keratitis |
| title_sort | comparison on the sensitivity of laboratory diagnosis technology in the diagnosis of fungal keratitis |
| topic | keratitis fungal fungal smear fungal culture pathological examination |
| url | http://ies.ijo.cn/cn_publish/2015/8/201508005.pdf |
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