MicroRNA-506 inhibits tumor growth and metastasis in nasopharyngeal carcinoma through the inactivation of the Wnt/β-catenin signaling pathway by down-regulating LHX2
Abstract Background Epithelial-mesenchymal transition (EMT)-associated proteins play key roles in cancer progression and metastasis with the involvement of microRNAs (miRNAs). This study aims to assess the role of miR-506 working in tandem with LIM Homeobox 2 (LHX2) in EMT and metastasis through the...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-02-01
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| Series: | Journal of Experimental & Clinical Cancer Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13046-019-1023-4 |
| _version_ | 1818320463220178944 |
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| author | Tian-Song Liang Ying-Juan Zheng Juan Wang Jing-Yi Zhao Dao-Ke Yang Zhang-Suo Liu |
| author_facet | Tian-Song Liang Ying-Juan Zheng Juan Wang Jing-Yi Zhao Dao-Ke Yang Zhang-Suo Liu |
| author_sort | Tian-Song Liang |
| collection | DOAJ |
| description | Abstract Background Epithelial-mesenchymal transition (EMT)-associated proteins play key roles in cancer progression and metastasis with the involvement of microRNAs (miRNAs). This study aims to assess the role of miR-506 working in tandem with LIM Homeobox 2 (LHX2) in EMT and metastasis through the Wnt/β-catenin signaling pathway in nasopharyngeal carcinoma (NPC). Methods Differentially expressed genes associated with NPC were screened using microarray analyses, from which LHX2 was identified. Next, the potential relationship between miR-506 and LHX2 was analyzed. In order to explore the effect of miR-506 or LHX2 on NPC cell proliferation, migration, invasion and apoptosis, serials of mimics, inhibitors or siRNA against LHX2 were transfected into NPC cells. Then, the expression patterns of LHX2, Wnt1, β-catenin, E-cadherin, Vimentin, TCF4 and Twist were determined to assess the influence of miR-506 or LHX2 on EMT as well as the relationship between the Wnt/β-catenin signaling pathway and TCF4. The tumorigenicity and lymph node metastasis (LNM) in xenograft tumors of nude mice were observed. Results The has-miR-506-3p was identified as the down-regulated gene in NPC based on the microarray data while LHX2 was negatively regulated by miR-506. Over-expression of miR-506 or silencing of LHK2 inhibited NPC cell proliferation, migration, invasion, tumorigenicity and LNM but promoted apoptosis indicated by decreased Wnt1, β-catenin, Vimentin, TCF4 and Twist expressions along with increased E-cadherin expressions. Conclusions miR-506 inhibits tumor growth and metastasis in NPC via inhibition of Wnt/β-catenin signaling by down-regulating LHX2, accompanied by decreased TCF4. Taken together, miR-506 targeted-inhibition LHX2 presents a promising therapeutic strategy for the treatment of NPC. Trial registration ChiCTR1800018889. Registered 15 October 2018. |
| first_indexed | 2024-12-13T10:25:24Z |
| format | Article |
| id | doaj.art-21c4f4c72388463aa08465eb6ff5321d |
| institution | Directory Open Access Journal |
| issn | 1756-9966 |
| language | English |
| last_indexed | 2024-12-13T10:25:24Z |
| publishDate | 2019-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Experimental & Clinical Cancer Research |
| spelling | doaj.art-21c4f4c72388463aa08465eb6ff5321d2022-12-21T23:51:03ZengBMCJournal of Experimental & Clinical Cancer Research1756-99662019-02-0138112010.1186/s13046-019-1023-4MicroRNA-506 inhibits tumor growth and metastasis in nasopharyngeal carcinoma through the inactivation of the Wnt/β-catenin signaling pathway by down-regulating LHX2Tian-Song Liang0Ying-Juan Zheng1Juan Wang2Jing-Yi Zhao3Dao-Ke Yang4Zhang-Suo Liu5Department of Radiotherapy, the First Affiliated Hospital of Zhengzhou UniversityDepartment of Radiotherapy, the First Affiliated Hospital of Zhengzhou UniversityDepartment of Radiotherapy, the First Affiliated Hospital of Zhengzhou UniversityDepartment of Radiotherapy, the First Affiliated Hospital of Zhengzhou UniversityDepartment of Radiotherapy, the First Affiliated Hospital of Zhengzhou UniversityDepartment of Radiotherapy, the First Affiliated Hospital of Zhengzhou UniversityAbstract Background Epithelial-mesenchymal transition (EMT)-associated proteins play key roles in cancer progression and metastasis with the involvement of microRNAs (miRNAs). This study aims to assess the role of miR-506 working in tandem with LIM Homeobox 2 (LHX2) in EMT and metastasis through the Wnt/β-catenin signaling pathway in nasopharyngeal carcinoma (NPC). Methods Differentially expressed genes associated with NPC were screened using microarray analyses, from which LHX2 was identified. Next, the potential relationship between miR-506 and LHX2 was analyzed. In order to explore the effect of miR-506 or LHX2 on NPC cell proliferation, migration, invasion and apoptosis, serials of mimics, inhibitors or siRNA against LHX2 were transfected into NPC cells. Then, the expression patterns of LHX2, Wnt1, β-catenin, E-cadherin, Vimentin, TCF4 and Twist were determined to assess the influence of miR-506 or LHX2 on EMT as well as the relationship between the Wnt/β-catenin signaling pathway and TCF4. The tumorigenicity and lymph node metastasis (LNM) in xenograft tumors of nude mice were observed. Results The has-miR-506-3p was identified as the down-regulated gene in NPC based on the microarray data while LHX2 was negatively regulated by miR-506. Over-expression of miR-506 or silencing of LHK2 inhibited NPC cell proliferation, migration, invasion, tumorigenicity and LNM but promoted apoptosis indicated by decreased Wnt1, β-catenin, Vimentin, TCF4 and Twist expressions along with increased E-cadherin expressions. Conclusions miR-506 inhibits tumor growth and metastasis in NPC via inhibition of Wnt/β-catenin signaling by down-regulating LHX2, accompanied by decreased TCF4. Taken together, miR-506 targeted-inhibition LHX2 presents a promising therapeutic strategy for the treatment of NPC. Trial registration ChiCTR1800018889. Registered 15 October 2018.http://link.springer.com/article/10.1186/s13046-019-1023-4MicroRNA-506LIM Homeobox 2Wnt/β-catenin signaling pathwayEpithelial-mesenchymal transitionLymph node metastasisNasopharyngeal carcinoma |
| spellingShingle | Tian-Song Liang Ying-Juan Zheng Juan Wang Jing-Yi Zhao Dao-Ke Yang Zhang-Suo Liu MicroRNA-506 inhibits tumor growth and metastasis in nasopharyngeal carcinoma through the inactivation of the Wnt/β-catenin signaling pathway by down-regulating LHX2 Journal of Experimental & Clinical Cancer Research MicroRNA-506 LIM Homeobox 2 Wnt/β-catenin signaling pathway Epithelial-mesenchymal transition Lymph node metastasis Nasopharyngeal carcinoma |
| title | MicroRNA-506 inhibits tumor growth and metastasis in nasopharyngeal carcinoma through the inactivation of the Wnt/β-catenin signaling pathway by down-regulating LHX2 |
| title_full | MicroRNA-506 inhibits tumor growth and metastasis in nasopharyngeal carcinoma through the inactivation of the Wnt/β-catenin signaling pathway by down-regulating LHX2 |
| title_fullStr | MicroRNA-506 inhibits tumor growth and metastasis in nasopharyngeal carcinoma through the inactivation of the Wnt/β-catenin signaling pathway by down-regulating LHX2 |
| title_full_unstemmed | MicroRNA-506 inhibits tumor growth and metastasis in nasopharyngeal carcinoma through the inactivation of the Wnt/β-catenin signaling pathway by down-regulating LHX2 |
| title_short | MicroRNA-506 inhibits tumor growth and metastasis in nasopharyngeal carcinoma through the inactivation of the Wnt/β-catenin signaling pathway by down-regulating LHX2 |
| title_sort | microrna 506 inhibits tumor growth and metastasis in nasopharyngeal carcinoma through the inactivation of the wnt β catenin signaling pathway by down regulating lhx2 |
| topic | MicroRNA-506 LIM Homeobox 2 Wnt/β-catenin signaling pathway Epithelial-mesenchymal transition Lymph node metastasis Nasopharyngeal carcinoma |
| url | http://link.springer.com/article/10.1186/s13046-019-1023-4 |
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