Cell Proteomic Footprinting: Advances in the Quality of Cellular and Cell-Derived Cancer Vaccines
In omics sciences, many compounds are measured simultaneously in a sample in a single run. Such analytical performance opens up prospects for improving cellular cancer vaccines and other cell-based immunotherapeutics. This article provides an overview of proteomics technology, known as cell proteomi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/2/661 |
| _version_ | 1797618653459382272 |
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| author | Petr G. Lokhov Elena E. Balashova Oxana P. Trifonova Dmitry L. Maslov Alexander I. Archakov |
| author_facet | Petr G. Lokhov Elena E. Balashova Oxana P. Trifonova Dmitry L. Maslov Alexander I. Archakov |
| author_sort | Petr G. Lokhov |
| collection | DOAJ |
| description | In omics sciences, many compounds are measured simultaneously in a sample in a single run. Such analytical performance opens up prospects for improving cellular cancer vaccines and other cell-based immunotherapeutics. This article provides an overview of proteomics technology, known as cell proteomic footprinting. The molecular phenotype of cells is highly variable, and their antigenic profile is affected by many factors, including cell isolation from the tissue, cell cultivation conditions, and storage procedures. This makes the therapeutic properties of cells, including those used in vaccines, unpredictable. Cell proteomic footprinting makes it possible to obtain controlled cell products. Namely, this technology facilitates the cell authentication and quality control of cells regarding their molecular phenotype, which is directly connected with the antigenic properties of cell products. Protocols for cell proteomic footprinting with their crucial moments, footprint processing, and recommendations for the implementation of this technology are described in this paper. The provided footprints in this paper and program source code for their processing contribute to the fast implementation of this technology in the development and manufacturing of cell-based immunotherapeutics. |
| first_indexed | 2024-03-11T08:15:34Z |
| format | Article |
| id | doaj.art-21c56e0ae8ad48a9af97652fa0bf5907 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-11T08:15:34Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-21c56e0ae8ad48a9af97652fa0bf59072023-11-16T22:42:56ZengMDPI AGPharmaceutics1999-49232023-02-0115266110.3390/pharmaceutics15020661Cell Proteomic Footprinting: Advances in the Quality of Cellular and Cell-Derived Cancer VaccinesPetr G. Lokhov0Elena E. Balashova1Oxana P. Trifonova2Dmitry L. Maslov3Alexander I. Archakov4Institute of Biomedical Chemistry, 10 Building 8, Pogodinskaya Street, 119121 Moscow, RussiaInstitute of Biomedical Chemistry, 10 Building 8, Pogodinskaya Street, 119121 Moscow, RussiaInstitute of Biomedical Chemistry, 10 Building 8, Pogodinskaya Street, 119121 Moscow, RussiaInstitute of Biomedical Chemistry, 10 Building 8, Pogodinskaya Street, 119121 Moscow, RussiaInstitute of Biomedical Chemistry, 10 Building 8, Pogodinskaya Street, 119121 Moscow, RussiaIn omics sciences, many compounds are measured simultaneously in a sample in a single run. Such analytical performance opens up prospects for improving cellular cancer vaccines and other cell-based immunotherapeutics. This article provides an overview of proteomics technology, known as cell proteomic footprinting. The molecular phenotype of cells is highly variable, and their antigenic profile is affected by many factors, including cell isolation from the tissue, cell cultivation conditions, and storage procedures. This makes the therapeutic properties of cells, including those used in vaccines, unpredictable. Cell proteomic footprinting makes it possible to obtain controlled cell products. Namely, this technology facilitates the cell authentication and quality control of cells regarding their molecular phenotype, which is directly connected with the antigenic properties of cell products. Protocols for cell proteomic footprinting with their crucial moments, footprint processing, and recommendations for the implementation of this technology are described in this paper. The provided footprints in this paper and program source code for their processing contribute to the fast implementation of this technology in the development and manufacturing of cell-based immunotherapeutics.https://www.mdpi.com/1999-4923/15/2/661proteomic footprintcancer vaccineantigensvaccinationcell authenticationquality control |
| spellingShingle | Petr G. Lokhov Elena E. Balashova Oxana P. Trifonova Dmitry L. Maslov Alexander I. Archakov Cell Proteomic Footprinting: Advances in the Quality of Cellular and Cell-Derived Cancer Vaccines Pharmaceutics proteomic footprint cancer vaccine antigens vaccination cell authentication quality control |
| title | Cell Proteomic Footprinting: Advances in the Quality of Cellular and Cell-Derived Cancer Vaccines |
| title_full | Cell Proteomic Footprinting: Advances in the Quality of Cellular and Cell-Derived Cancer Vaccines |
| title_fullStr | Cell Proteomic Footprinting: Advances in the Quality of Cellular and Cell-Derived Cancer Vaccines |
| title_full_unstemmed | Cell Proteomic Footprinting: Advances in the Quality of Cellular and Cell-Derived Cancer Vaccines |
| title_short | Cell Proteomic Footprinting: Advances in the Quality of Cellular and Cell-Derived Cancer Vaccines |
| title_sort | cell proteomic footprinting advances in the quality of cellular and cell derived cancer vaccines |
| topic | proteomic footprint cancer vaccine antigens vaccination cell authentication quality control |
| url | https://www.mdpi.com/1999-4923/15/2/661 |
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