New Generation of Hybrid Materials Based on Gelatin and Bioactive Glass Particles for Bone Tissue Regeneration
Hybrid scaffolds based on bioactive glass (BAG) particles (<38 µm), covalently linked to gelatin (G*) using 3-glycidoxypropyltrimethoxysilane (GPTMS), have been studied for bone bioengineering. In this study, two glass compositions (13-93 and 13-93B20 (where 20% of the SiO<sub>2</sub>...
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| Format: | Article |
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MDPI AG
2021-03-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/3/444 |
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| author | Amel Houaoui Agata Szczodra Mari Lallukka Lamia El-Guermah Remy Agniel Emmanuel Pauthe Jonathan Massera Michel Boissiere |
| author_facet | Amel Houaoui Agata Szczodra Mari Lallukka Lamia El-Guermah Remy Agniel Emmanuel Pauthe Jonathan Massera Michel Boissiere |
| author_sort | Amel Houaoui |
| collection | DOAJ |
| description | Hybrid scaffolds based on bioactive glass (BAG) particles (<38 µm), covalently linked to gelatin (G*) using 3-glycidoxypropyltrimethoxysilane (GPTMS), have been studied for bone bioengineering. In this study, two glass compositions (13-93 and 13-93B20 (where 20% of the SiO<sub>2</sub> was replaced with B<sub>2</sub>O<sub>3</sub>)) were introduced in the gelatin matrix. The C<sub>factor</sub> (gelatin/GPTMS molar ratio) was kept constant at 500. The hybrids obtained were found to be stable at 37 °C in solution, the condition in which pure gelatin is liquid. All hybrids were characterized by in vitro dissolution in Tris(hydroxymethyl)aminomethane (TRIS) solution (for up to 4 weeks) and Simulated Body Fluid (SBF) (for up to 2 weeks). Samples processed with 13-93B20 exhibited faster initial dissolution and significantly faster precipitation of a hydroxyapatite (HA) layer. The faster ion release and HA precipitation recorded from the G*/13-93B20 samples are attributable to the higher reactivity of borosilicate compared to silicate glass. The MC3T3-E1 cell behavior in direct contact with the hybrids was investigated, showing that the cells were able to proliferate and spread on the developed biomaterials. Tailoring the glass composition allows us to better control the material’s dissolution, biodegradability, and bioactivity. Bioactive (especially with 13-93B20 BAG) and biocompatible, the hybrids are promising for bone application. |
| first_indexed | 2024-03-10T13:10:17Z |
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| id | doaj.art-21c89fbb87b341198640f8650cb39be9 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T13:10:17Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-21c89fbb87b341198640f8650cb39be92023-11-21T10:49:16ZengMDPI AGBiomolecules2218-273X2021-03-0111344410.3390/biom11030444New Generation of Hybrid Materials Based on Gelatin and Bioactive Glass Particles for Bone Tissue RegenerationAmel Houaoui0Agata Szczodra1Mari Lallukka2Lamia El-Guermah3Remy Agniel4Emmanuel Pauthe5Jonathan Massera6Michel Boissiere7Biomaterials for Health Research Group, ERRMECe, Equipe de Recherche sur les Relations Matrice Extracellulaire-Cellules (EA1391), Institut des Matériaux I-MAT (FD4122), CY Tech, CY Cergy Paris Université, Maison Internationale de la Recherche (MIR), rue Descartes, 95001 Neuville sur Oise CEDEX, FranceTampere University, Faculty of Medicine and Health Technology, Laboratory of Biomaterials and Tissue Engineering, Korkeakoulunkatu 3, 33720 Tampere, FinlandTampere University, Faculty of Medicine and Health Technology, Laboratory of Biomaterials and Tissue Engineering, Korkeakoulunkatu 3, 33720 Tampere, FinlandBiomaterials for Health Research Group, ERRMECe, Equipe de Recherche sur les Relations Matrice Extracellulaire-Cellules (EA1391), Institut des Matériaux I-MAT (FD4122), CY Tech, CY Cergy Paris Université, Maison Internationale de la Recherche (MIR), rue Descartes, 95001 Neuville sur Oise CEDEX, FranceBiomaterials for Health Research Group, ERRMECe, Equipe de Recherche sur les Relations Matrice Extracellulaire-Cellules (EA1391), Institut des Matériaux I-MAT (FD4122), CY Tech, CY Cergy Paris Université, Maison Internationale de la Recherche (MIR), rue Descartes, 95001 Neuville sur Oise CEDEX, FranceBiomaterials for Health Research Group, ERRMECe, Equipe de Recherche sur les Relations Matrice Extracellulaire-Cellules (EA1391), Institut des Matériaux I-MAT (FD4122), CY Tech, CY Cergy Paris Université, Maison Internationale de la Recherche (MIR), rue Descartes, 95001 Neuville sur Oise CEDEX, FranceTampere University, Faculty of Medicine and Health Technology, Laboratory of Biomaterials and Tissue Engineering, Korkeakoulunkatu 3, 33720 Tampere, FinlandBiomaterials for Health Research Group, ERRMECe, Equipe de Recherche sur les Relations Matrice Extracellulaire-Cellules (EA1391), Institut des Matériaux I-MAT (FD4122), CY Tech, CY Cergy Paris Université, Maison Internationale de la Recherche (MIR), rue Descartes, 95001 Neuville sur Oise CEDEX, FranceHybrid scaffolds based on bioactive glass (BAG) particles (<38 µm), covalently linked to gelatin (G*) using 3-glycidoxypropyltrimethoxysilane (GPTMS), have been studied for bone bioengineering. In this study, two glass compositions (13-93 and 13-93B20 (where 20% of the SiO<sub>2</sub> was replaced with B<sub>2</sub>O<sub>3</sub>)) were introduced in the gelatin matrix. The C<sub>factor</sub> (gelatin/GPTMS molar ratio) was kept constant at 500. The hybrids obtained were found to be stable at 37 °C in solution, the condition in which pure gelatin is liquid. All hybrids were characterized by in vitro dissolution in Tris(hydroxymethyl)aminomethane (TRIS) solution (for up to 4 weeks) and Simulated Body Fluid (SBF) (for up to 2 weeks). Samples processed with 13-93B20 exhibited faster initial dissolution and significantly faster precipitation of a hydroxyapatite (HA) layer. The faster ion release and HA precipitation recorded from the G*/13-93B20 samples are attributable to the higher reactivity of borosilicate compared to silicate glass. The MC3T3-E1 cell behavior in direct contact with the hybrids was investigated, showing that the cells were able to proliferate and spread on the developed biomaterials. Tailoring the glass composition allows us to better control the material’s dissolution, biodegradability, and bioactivity. Bioactive (especially with 13-93B20 BAG) and biocompatible, the hybrids are promising for bone application.https://www.mdpi.com/2218-273X/11/3/444hybrid scaffoldbioactive glassgelatinGPTMSbone tissue engineering |
| spellingShingle | Amel Houaoui Agata Szczodra Mari Lallukka Lamia El-Guermah Remy Agniel Emmanuel Pauthe Jonathan Massera Michel Boissiere New Generation of Hybrid Materials Based on Gelatin and Bioactive Glass Particles for Bone Tissue Regeneration Biomolecules hybrid scaffold bioactive glass gelatin GPTMS bone tissue engineering |
| title | New Generation of Hybrid Materials Based on Gelatin and Bioactive Glass Particles for Bone Tissue Regeneration |
| title_full | New Generation of Hybrid Materials Based on Gelatin and Bioactive Glass Particles for Bone Tissue Regeneration |
| title_fullStr | New Generation of Hybrid Materials Based on Gelatin and Bioactive Glass Particles for Bone Tissue Regeneration |
| title_full_unstemmed | New Generation of Hybrid Materials Based on Gelatin and Bioactive Glass Particles for Bone Tissue Regeneration |
| title_short | New Generation of Hybrid Materials Based on Gelatin and Bioactive Glass Particles for Bone Tissue Regeneration |
| title_sort | new generation of hybrid materials based on gelatin and bioactive glass particles for bone tissue regeneration |
| topic | hybrid scaffold bioactive glass gelatin GPTMS bone tissue engineering |
| url | https://www.mdpi.com/2218-273X/11/3/444 |
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