Actividad antibacteriana de extracto de hojas de Melia azedarach L
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| Format: | Article |
| Language: | Spanish |
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Universidad Nacional de Colombia
2012-09-01
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| Series: | Revista Colombiana de Biotecnología |
| Subjects: | |
| Online Access: | http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/32883 |
| _version_ | 1818671909081972736 |
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| author | Johanna Norela Rojas Sierra Alexander Francisco Pérez Cordero Jose Gregorio Martínez Avilez Jorge Ulises Mieles Galindo |
| author_facet | Johanna Norela Rojas Sierra Alexander Francisco Pérez Cordero Jose Gregorio Martínez Avilez Jorge Ulises Mieles Galindo |
| author_sort | Johanna Norela Rojas Sierra |
| collection | DOAJ |
| description | <!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:TrackMoves /> <w:TrackFormatting /> <w:HyphenationZone>21</w:HyphenationZone> <w:PunctuationKerning /> <w:ValidateAgainstSchemas /> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:DoNotPromoteQF /> <w:LidThemeOther>ES-CO</w:LidThemeOther> <w:LidThemeAsian>X-NONE</w:LidThemeAsian> <w:LidThemeComplexScript>X-NONE</w:LidThemeComplexScript> <w:Compatibility> <w:BreakWrappedTables /> <w:SnapToGridInCell /> <w:WrapTextWithPunct /> <w:UseAsianBreakRules /> <w:DontGrowAutofit /> <w:SplitPgBreakAndParaMark /> <w:DontVertAlignCellWithSp /> <w:DontBreakConstrainedForcedTables /> <w:DontVertAlignInTxbx /> <w:Word11KerningPairs /> <w:CachedColBalance /> </w:Compatibility> <w:BrowserLevel>MicrosoftInternetExplorer4</w:BrowserLevel> <m:mathPr> <m:mathFont m:val="Cambria Math" /> <m:brkBin m:val="before" /> <m:brkBinSub m:val=" " /> <m:smallFrac m:val="off" /> <m:dispDef /> <m:lMargin m:val="0" /> <m:rMargin m:val="0" /> <m:defJc m:val="centerGroup" /> <m:wrapIndent m:val="1440" /> <m:intLim m:val="subSup" /> <m:naryLim m:val="undOvr" /> </m:mathPr></w:WordDocument> </xml><![endif]--><strong><span style="mso-ansi-language: EN-US;" lang="EN-US">Título en ingles: Antibacterial activity of leaf extract <em>Melia azedarach</em> L.</span></strong> <p class="MsoNormal" style="text-align: justify;"><strong><span lang="ES">Resumen: </span></strong><span lang="ES">El trabajo tuvo como objetivo evaluar la actividad antibacteriana de extractos de hojas de <em>Melia azedarach</em> L. sobre seis bacterias patógenas. Inicialmente mediante extracción por el método Soxhlet se obtuvo extracto total en etanol y a partir de éste se prepararon fracciones líquido-líquido con éter de petróleo y acetato de etilo. El extracto total y las dos fracciones fueron diluidos a diferentes concentraciones (ppm) para evaluar <em style="mso-bidi-font-style: normal;">in vitro</em> su actividad antibacteriana. <span style="mso-spacerun: yes;"> </span>Las bacterias de mayor susceptibilidad fueron</span><span style="mso-fareast-language: ES-CO;" lang="ES"> las patógenas de humanos </span><em><span lang="ES">Enterococcus faecalis</span></em><span lang="ES">, </span><em><span style="mso-fareast-language: EN-US;" lang="ES">Escherichia coli, </span><span lang="ES">Pseudomonas aeruginosa</span></em><span style="mso-fareast-language: EN-US;" lang="ES"> y </span><em><span lang="ES">Klebsiella oxytoca</span></em><span lang="ES">, en relación a la fitopatógena <em>Burkholderia glumae </em>quien mostró resistencia a todos los tratamientos. Las bacterias patógenas fueron más susceptibles a la fracción éter de petróleo a concentración mínima de 25 ppm. El screen fitoquímico de la planta indicó presencia de metabolitos secundarios tipo alcaloides, terpenos/esteroles, saponinas, taninos y antocianinas. Estos resultados evidencian el posible uso de <em style="mso-bidi-font-style: normal;">M. azederach</em> como alternativa de control biológico sobre las bacterias analizadas.</span></p> <p class="MsoNormal" style="text-align: justify;"><strong style="mso-bidi-font-weight: normal;"><span lang="ES">Palabras clave</span></strong><span lang="ES">: bacterias; patógenas; inhibición; extractos.</span></p> <p class="MsoNormal" style="text-align: justify;"><strong><span style="mso-ansi-language: EN-GB;" lang="EN-GB">Abstract: </span></strong><span style="mso-ansi-language: EN-US;" lang="EN-US">The work was objective to evaluate the antibacterial activity of extracts from leaves of <em style="mso-bidi-font-style: normal;">Melia azedarach</em> (L) on six pathogenic bacteria. Total ethanol extract was obtained initially by extraction with method Soxhlet and from this prepared liquid-liquid fractions with petroleum ether and ethyl acetate. The total extract and the two fractions were diluted at different concentrations (ppm) to evaluate its antibacterial activity in vitro.<span style="mso-spacerun: yes;"> </span>More susceptible bacteria were the pathogenic human <em style="mso-bidi-font-style: normal;">Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa</em> and <em style="mso-bidi-font-style: normal;">Klebsiella oxytoca</em>, in relation to the plant pathogen <em style="mso-bidi-font-style: normal;">Burkholderia glumae</em> who showed resistance to all treatments. Pathogenic bacteria were more susceptible to the fraction of petroleum ether to the minimum concentration of 25 ppm. Screen plant phytochemical indicated presence of secondary metabolites type alkaloids, terpenes/sterols, saponins, tannins and anthocyanins. These results demonstrate the potential use of <em style="mso-bidi-font-style: normal;">M. azederach </em>as biological control alternatively on analysed bacterial.</span></p> <p class="MsoNormal" style="text-align: justify;"><strong style="mso-bidi-font-weight: normal;"><span style="mso-ansi-language: EN-US;" lang="EN-US">Key words</span></strong><span style="mso-ansi-language: EN-US;" lang="EN-US">: bacteria; pathogenic; inhibition; extract.</span></p> |
| first_indexed | 2024-12-17T07:31:29Z |
| format | Article |
| id | doaj.art-21ca311eb58348b69211c917f86468e3 |
| institution | Directory Open Access Journal |
| issn | 0123-3475 1909-8758 |
| language | Spanish |
| last_indexed | 2024-12-17T07:31:29Z |
| publishDate | 2012-09-01 |
| publisher | Universidad Nacional de Colombia |
| record_format | Article |
| series | Revista Colombiana de Biotecnología |
| spelling | doaj.art-21ca311eb58348b69211c917f86468e32022-12-21T21:58:28ZspaUniversidad Nacional de ColombiaRevista Colombiana de Biotecnología0123-34751909-87582012-09-0114122423228995Actividad antibacteriana de extracto de hojas de Melia azedarach LJohanna Norela Rojas Sierra0Alexander Francisco Pérez Cordero1Jose Gregorio Martínez Avilez2Jorge Ulises Mieles Galindo3investigadorinvestigadorinvestigadorinvestigador<!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:TrackMoves /> <w:TrackFormatting /> <w:HyphenationZone>21</w:HyphenationZone> <w:PunctuationKerning /> <w:ValidateAgainstSchemas /> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:DoNotPromoteQF /> <w:LidThemeOther>ES-CO</w:LidThemeOther> <w:LidThemeAsian>X-NONE</w:LidThemeAsian> <w:LidThemeComplexScript>X-NONE</w:LidThemeComplexScript> <w:Compatibility> <w:BreakWrappedTables /> <w:SnapToGridInCell /> <w:WrapTextWithPunct /> <w:UseAsianBreakRules /> <w:DontGrowAutofit /> <w:SplitPgBreakAndParaMark /> <w:DontVertAlignCellWithSp /> <w:DontBreakConstrainedForcedTables /> <w:DontVertAlignInTxbx /> <w:Word11KerningPairs /> <w:CachedColBalance /> </w:Compatibility> <w:BrowserLevel>MicrosoftInternetExplorer4</w:BrowserLevel> <m:mathPr> <m:mathFont m:val="Cambria Math" /> <m:brkBin m:val="before" /> <m:brkBinSub m:val=" " /> <m:smallFrac m:val="off" /> <m:dispDef /> <m:lMargin m:val="0" /> <m:rMargin m:val="0" /> <m:defJc m:val="centerGroup" /> <m:wrapIndent m:val="1440" /> <m:intLim m:val="subSup" /> <m:naryLim m:val="undOvr" /> </m:mathPr></w:WordDocument> </xml><![endif]--><strong><span style="mso-ansi-language: EN-US;" lang="EN-US">Título en ingles: Antibacterial activity of leaf extract <em>Melia azedarach</em> L.</span></strong> <p class="MsoNormal" style="text-align: justify;"><strong><span lang="ES">Resumen: </span></strong><span lang="ES">El trabajo tuvo como objetivo evaluar la actividad antibacteriana de extractos de hojas de <em>Melia azedarach</em> L. sobre seis bacterias patógenas. Inicialmente mediante extracción por el método Soxhlet se obtuvo extracto total en etanol y a partir de éste se prepararon fracciones líquido-líquido con éter de petróleo y acetato de etilo. El extracto total y las dos fracciones fueron diluidos a diferentes concentraciones (ppm) para evaluar <em style="mso-bidi-font-style: normal;">in vitro</em> su actividad antibacteriana. <span style="mso-spacerun: yes;"> </span>Las bacterias de mayor susceptibilidad fueron</span><span style="mso-fareast-language: ES-CO;" lang="ES"> las patógenas de humanos </span><em><span lang="ES">Enterococcus faecalis</span></em><span lang="ES">, </span><em><span style="mso-fareast-language: EN-US;" lang="ES">Escherichia coli, </span><span lang="ES">Pseudomonas aeruginosa</span></em><span style="mso-fareast-language: EN-US;" lang="ES"> y </span><em><span lang="ES">Klebsiella oxytoca</span></em><span lang="ES">, en relación a la fitopatógena <em>Burkholderia glumae </em>quien mostró resistencia a todos los tratamientos. Las bacterias patógenas fueron más susceptibles a la fracción éter de petróleo a concentración mínima de 25 ppm. El screen fitoquímico de la planta indicó presencia de metabolitos secundarios tipo alcaloides, terpenos/esteroles, saponinas, taninos y antocianinas. Estos resultados evidencian el posible uso de <em style="mso-bidi-font-style: normal;">M. azederach</em> como alternativa de control biológico sobre las bacterias analizadas.</span></p> <p class="MsoNormal" style="text-align: justify;"><strong style="mso-bidi-font-weight: normal;"><span lang="ES">Palabras clave</span></strong><span lang="ES">: bacterias; patógenas; inhibición; extractos.</span></p> <p class="MsoNormal" style="text-align: justify;"><strong><span style="mso-ansi-language: EN-GB;" lang="EN-GB">Abstract: </span></strong><span style="mso-ansi-language: EN-US;" lang="EN-US">The work was objective to evaluate the antibacterial activity of extracts from leaves of <em style="mso-bidi-font-style: normal;">Melia azedarach</em> (L) on six pathogenic bacteria. Total ethanol extract was obtained initially by extraction with method Soxhlet and from this prepared liquid-liquid fractions with petroleum ether and ethyl acetate. The total extract and the two fractions were diluted at different concentrations (ppm) to evaluate its antibacterial activity in vitro.<span style="mso-spacerun: yes;"> </span>More susceptible bacteria were the pathogenic human <em style="mso-bidi-font-style: normal;">Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa</em> and <em style="mso-bidi-font-style: normal;">Klebsiella oxytoca</em>, in relation to the plant pathogen <em style="mso-bidi-font-style: normal;">Burkholderia glumae</em> who showed resistance to all treatments. Pathogenic bacteria were more susceptible to the fraction of petroleum ether to the minimum concentration of 25 ppm. Screen plant phytochemical indicated presence of secondary metabolites type alkaloids, terpenes/sterols, saponins, tannins and anthocyanins. These results demonstrate the potential use of <em style="mso-bidi-font-style: normal;">M. azederach </em>as biological control alternatively on analysed bacterial.</span></p> <p class="MsoNormal" style="text-align: justify;"><strong style="mso-bidi-font-weight: normal;"><span style="mso-ansi-language: EN-US;" lang="EN-US">Key words</span></strong><span style="mso-ansi-language: EN-US;" lang="EN-US">: bacteria; pathogenic; inhibition; extract.</span></p>http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/32883bacteriaspatógenasinhibiciónextractosbacteriapathogenicinhibitionextract |
| spellingShingle | Johanna Norela Rojas Sierra Alexander Francisco Pérez Cordero Jose Gregorio Martínez Avilez Jorge Ulises Mieles Galindo Actividad antibacteriana de extracto de hojas de Melia azedarach L Revista Colombiana de Biotecnología bacterias patógenas inhibición extractos bacteria pathogenic inhibition extract |
| title | Actividad antibacteriana de extracto de hojas de Melia azedarach L |
| title_full | Actividad antibacteriana de extracto de hojas de Melia azedarach L |
| title_fullStr | Actividad antibacteriana de extracto de hojas de Melia azedarach L |
| title_full_unstemmed | Actividad antibacteriana de extracto de hojas de Melia azedarach L |
| title_short | Actividad antibacteriana de extracto de hojas de Melia azedarach L |
| title_sort | actividad antibacteriana de extracto de hojas de melia azedarach l |
| topic | bacterias patógenas inhibición extractos bacteria pathogenic inhibition extract |
| url | http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/32883 |
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