Pycnogenol a promising remedy for diabetic keratopathy in experimentally induced corneal alkali burns in diabetic rats
Abstract Aim This study aimed to investigate the efficiency of topically applied pycnogenol (PYC) in healing the standardized alkaline corneal ulcer in diabetic and normal rats. Materials and methods The corneal alkali-burn injury (CA-I) model was unilaterally developed in Wistar rats by filter pape...
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| Format: | Article |
| Language: | English |
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BMC
2022-05-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-022-03307-3 |
| _version_ | 1811256996053123072 |
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| author | Mohamed A. Hamed Amany Farag Ibrahim S. Zahran Ahmed Hafez Mohamed Abdo Rizk Marwa Abass |
| author_facet | Mohamed A. Hamed Amany Farag Ibrahim S. Zahran Ahmed Hafez Mohamed Abdo Rizk Marwa Abass |
| author_sort | Mohamed A. Hamed |
| collection | DOAJ |
| description | Abstract Aim This study aimed to investigate the efficiency of topically applied pycnogenol (PYC) in healing the standardized alkaline corneal ulcer in diabetic and normal rats. Materials and methods The corneal alkali-burn injury (CA-I) model was unilaterally developed in Wistar rats by filter paper saturated with 0.01 M of NaOH and touching the eyes for 45 s. Rats were divided into four groups: Normal control (NC), normal PYC (NPYC), diabetic control (DC), and diabetic PYC (DPYC). Both NPYC and DPYC groups were daily treated with PY eye drops three times, whereas NC and DC ones were treated with ordinary saline for six successive days. Results The wound healing of corneal epithelial was improved in the NPYC group compared to the NC group. Meanwhile, it was significantly improved (P < 0.05) in the DPYC group than in the DC group. Histological examination revealed that corneal re-epithelialization was more accomplished in the DPYC group than in the DC group. In addition, the inflammatory cells were augmented in the DC group more than those in the DPYC one. Conclusion The findings obtained revealed the efficiency of PYC for enhancing the corneal re-epithelialization and reducing the inflammatory reaction post alkali burn in rats, and thus it could be beneficially valuable as a treatment for the diabetic keratopathy. |
| first_indexed | 2024-04-12T17:49:38Z |
| format | Article |
| id | doaj.art-21ca9c8b0f4e4723ab2c4007a3a38791 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-04-12T17:49:38Z |
| publishDate | 2022-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-21ca9c8b0f4e4723ab2c4007a3a387912022-12-22T03:22:32ZengBMCBMC Veterinary Research1746-61482022-05-0118111010.1186/s12917-022-03307-3Pycnogenol a promising remedy for diabetic keratopathy in experimentally induced corneal alkali burns in diabetic ratsMohamed A. Hamed0Amany Farag1Ibrahim S. Zahran2Ahmed Hafez3Mohamed Abdo Rizk4Marwa Abass5Department of Surgery, Anesthesiology, and Radiology, Faculty of Veterinary Medicine, Aswan UniversityDepartment of Cytology and Histology, Faculty of Veterinary Medicine, Mansoura UniversityDepartment of Physiology, Faculty of Veterinary Medicine, Aswan UniversityDepartment of Pharmacology, Faculty of Veterinary Medicine, Aswan UniversityDepartment of Internal Medicine, Infectious and Fish Diseases, Faculty of Veterinary Medicine, Mansoura UniversityDepartment of Surgery, Anesthesiology, and Radiology, Faculty of Veterinary Medicine, Mansoura UniversityAbstract Aim This study aimed to investigate the efficiency of topically applied pycnogenol (PYC) in healing the standardized alkaline corneal ulcer in diabetic and normal rats. Materials and methods The corneal alkali-burn injury (CA-I) model was unilaterally developed in Wistar rats by filter paper saturated with 0.01 M of NaOH and touching the eyes for 45 s. Rats were divided into four groups: Normal control (NC), normal PYC (NPYC), diabetic control (DC), and diabetic PYC (DPYC). Both NPYC and DPYC groups were daily treated with PY eye drops three times, whereas NC and DC ones were treated with ordinary saline for six successive days. Results The wound healing of corneal epithelial was improved in the NPYC group compared to the NC group. Meanwhile, it was significantly improved (P < 0.05) in the DPYC group than in the DC group. Histological examination revealed that corneal re-epithelialization was more accomplished in the DPYC group than in the DC group. In addition, the inflammatory cells were augmented in the DC group more than those in the DPYC one. Conclusion The findings obtained revealed the efficiency of PYC for enhancing the corneal re-epithelialization and reducing the inflammatory reaction post alkali burn in rats, and thus it could be beneficially valuable as a treatment for the diabetic keratopathy.https://doi.org/10.1186/s12917-022-03307-3PycnogenolCorneaEpitheliumDiabetesWound healing |
| spellingShingle | Mohamed A. Hamed Amany Farag Ibrahim S. Zahran Ahmed Hafez Mohamed Abdo Rizk Marwa Abass Pycnogenol a promising remedy for diabetic keratopathy in experimentally induced corneal alkali burns in diabetic rats BMC Veterinary Research Pycnogenol Cornea Epithelium Diabetes Wound healing |
| title | Pycnogenol a promising remedy for diabetic keratopathy in experimentally induced corneal alkali burns in diabetic rats |
| title_full | Pycnogenol a promising remedy for diabetic keratopathy in experimentally induced corneal alkali burns in diabetic rats |
| title_fullStr | Pycnogenol a promising remedy for diabetic keratopathy in experimentally induced corneal alkali burns in diabetic rats |
| title_full_unstemmed | Pycnogenol a promising remedy for diabetic keratopathy in experimentally induced corneal alkali burns in diabetic rats |
| title_short | Pycnogenol a promising remedy for diabetic keratopathy in experimentally induced corneal alkali burns in diabetic rats |
| title_sort | pycnogenol a promising remedy for diabetic keratopathy in experimentally induced corneal alkali burns in diabetic rats |
| topic | Pycnogenol Cornea Epithelium Diabetes Wound healing |
| url | https://doi.org/10.1186/s12917-022-03307-3 |
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