Uterine Dendritic Cells Modulation by Mesenchymal Stem Cells Provides A Protective Microenvironment at The Feto-Maternal Interface: Improved Pregnancy Outcome in Abortion-Prone Mice
Objective: Dendritic cells (DCs) as major regulators of the immune response in the decidua play a pivotal role in establishment and maintenance of pregnancy. Immunological disorders are considered to be the main causes of unexplained recurrent spontaneous abortions (RSAs). Recently, we reported th...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Royan Institute (ACECR), Tehran
2019-06-01
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| Series: | Cell Journal |
| Subjects: | |
| Online Access: | https://celljournal.org/journal/article/abstract/6239 |
| Summary: | Objective: Dendritic cells (DCs) as major regulators of the immune response in the decidua play a pivotal role in
establishment and maintenance of pregnancy. Immunological disorders are considered to be the main causes of
unexplained recurrent spontaneous abortions (RSAs). Recently, we reported that mesenchymal stem cells (MSCs)
therapy could improve fetal survival and reduce the abortion rate in abortion-prone mice, although the precise
mechanisms of this action are poorly understood. Since MSCs have been shown to exert immunomodulatory effects
on the immune cells, especially DCs, this study was performed to investigate the capability of MSCs to modulate the
frequency, maturation state, and phenotype of uterine DCs (uDCs) as a potential mechanism for the improvement of
pregnancy outcome.
Materials and Methods: In this experimental study, adipose-derived MSCs were intraperitoneally administered to
abortion-prone pregnant mice on the fourth day of gestation. On the day 13.5 of pregnancy, after the determination of
abortion rates, the frequency, phenotype, and maturation state of uDCs were analyzed using flow cytometry.
Results: Our results indicated that the administration of MSCs, at the implantation window, could significantly decrease
the abortion rate and besides, increase the frequency of uDCs. MSCs administration also remarkably decreased the
expression of DCs maturation markers (MHC-II, CD86, and CD40) on uDCs. However, we did not find any difference
in the expression of CD11b on uDCs in MSCs-treated compared to control mice.
Conclusion: Regarding the mutual role of uDCs in establishment of a particular immunological state required for
appropriate implantation, proper maternal immune responses and development of successful pregnancy, it seems that
the modulation of uDCs by MSCs could be considered as one of the main mechanisms responsible for the positive
effect of MSCs on treatment of RSA. |
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| ISSN: | 2228-5806 2228-5814 |