Sperm Proteome after Interaction with Reproductive Fluids in Porcine: From the Ejaculation to the Fertilization Site
Ejaculated sperm are exposed to different environments before encountering the oocyte. However, how the sperm proteome changes during this transit remains unsolved. This study aimed to identify proteomic changes in boar sperm after incubation with male (seminal plasma, SP) and/or female (uterine flu...
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MDPI AG
2020-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/17/6060 |
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| author | Chiara Luongo Leopoldo González-Brusi Paula Cots-Rodríguez Mª José Izquierdo-Rico Manuel Avilés Francisco Alberto García-Vázquez |
| author_facet | Chiara Luongo Leopoldo González-Brusi Paula Cots-Rodríguez Mª José Izquierdo-Rico Manuel Avilés Francisco Alberto García-Vázquez |
| author_sort | Chiara Luongo |
| collection | DOAJ |
| description | Ejaculated sperm are exposed to different environments before encountering the oocyte. However, how the sperm proteome changes during this transit remains unsolved. This study aimed to identify proteomic changes in boar sperm after incubation with male (seminal plasma, SP) and/or female (uterine fluid, UF; and oviductal fluid, OF) reproductive fluids. The following experimental groups were analyzed: (1) SP: sperm + 20% SP; (2) UF: sperm + 20% UF; (3) OF: sperm + 20% OF; (4) SP + UF: sperm + 20% SP + 20% UF; and (5) SP+OF: sperm + 20% SP + 20% OF. The proteome analysis, performed by HPLC-MS/MS, allowed the identification of 265 proteins. A total of 69 proteins were detected in the UF, SP, and SP + UF groups, and 102 proteins in the OF, SP, and SP + OF groups. Our results showed a higher number of proteins when sperm were incubated with only one fluid than when they were co-incubated with two fluids. Additionally, the number of sperm-interacting proteins from the UF group was lower than the OF group. In conclusion, the interaction of sperm with reproductive fluids alters its proteome. The description of sperm-interacting proteins in porcine species after co-incubation with male and/or female reproductive fluids may be useful to understand sperm transport, selection, capacitation, or fertilization phenomena. |
| first_indexed | 2024-03-10T16:58:39Z |
| format | Article |
| id | doaj.art-21ce7522721d40db8d3239fa9001ad12 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T16:58:39Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-21ce7522721d40db8d3239fa9001ad122023-11-20T11:02:56ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-08-012117606010.3390/ijms21176060Sperm Proteome after Interaction with Reproductive Fluids in Porcine: From the Ejaculation to the Fertilization SiteChiara Luongo0Leopoldo González-Brusi1Paula Cots-Rodríguez2Mª José Izquierdo-Rico3Manuel Avilés4Francisco Alberto García-Vázquez5Department of Physiology, Veterinary School, International Excellence Campus for Higher Education and Research (Campus Mare Nostrum), University of Murcia, 30100 Murcia, SpainDepartment of Cell Biology and Histology, Faculty of Medicine, University of Murcia, 30100 Murcia, SpainDepartment of Cell Biology and Histology, Faculty of Medicine, University of Murcia, 30100 Murcia, SpainDepartment of Cell Biology and Histology, Faculty of Medicine, University of Murcia, 30100 Murcia, SpainDepartment of Cell Biology and Histology, Faculty of Medicine, University of Murcia, 30100 Murcia, SpainDepartment of Physiology, Veterinary School, International Excellence Campus for Higher Education and Research (Campus Mare Nostrum), University of Murcia, 30100 Murcia, SpainEjaculated sperm are exposed to different environments before encountering the oocyte. However, how the sperm proteome changes during this transit remains unsolved. This study aimed to identify proteomic changes in boar sperm after incubation with male (seminal plasma, SP) and/or female (uterine fluid, UF; and oviductal fluid, OF) reproductive fluids. The following experimental groups were analyzed: (1) SP: sperm + 20% SP; (2) UF: sperm + 20% UF; (3) OF: sperm + 20% OF; (4) SP + UF: sperm + 20% SP + 20% UF; and (5) SP+OF: sperm + 20% SP + 20% OF. The proteome analysis, performed by HPLC-MS/MS, allowed the identification of 265 proteins. A total of 69 proteins were detected in the UF, SP, and SP + UF groups, and 102 proteins in the OF, SP, and SP + OF groups. Our results showed a higher number of proteins when sperm were incubated with only one fluid than when they were co-incubated with two fluids. Additionally, the number of sperm-interacting proteins from the UF group was lower than the OF group. In conclusion, the interaction of sperm with reproductive fluids alters its proteome. The description of sperm-interacting proteins in porcine species after co-incubation with male and/or female reproductive fluids may be useful to understand sperm transport, selection, capacitation, or fertilization phenomena.https://www.mdpi.com/1422-0067/21/17/6060pigspermatozoabiofluidsfertilityproteomics |
| spellingShingle | Chiara Luongo Leopoldo González-Brusi Paula Cots-Rodríguez Mª José Izquierdo-Rico Manuel Avilés Francisco Alberto García-Vázquez Sperm Proteome after Interaction with Reproductive Fluids in Porcine: From the Ejaculation to the Fertilization Site International Journal of Molecular Sciences pig spermatozoa biofluids fertility proteomics |
| title | Sperm Proteome after Interaction with Reproductive Fluids in Porcine: From the Ejaculation to the Fertilization Site |
| title_full | Sperm Proteome after Interaction with Reproductive Fluids in Porcine: From the Ejaculation to the Fertilization Site |
| title_fullStr | Sperm Proteome after Interaction with Reproductive Fluids in Porcine: From the Ejaculation to the Fertilization Site |
| title_full_unstemmed | Sperm Proteome after Interaction with Reproductive Fluids in Porcine: From the Ejaculation to the Fertilization Site |
| title_short | Sperm Proteome after Interaction with Reproductive Fluids in Porcine: From the Ejaculation to the Fertilization Site |
| title_sort | sperm proteome after interaction with reproductive fluids in porcine from the ejaculation to the fertilization site |
| topic | pig spermatozoa biofluids fertility proteomics |
| url | https://www.mdpi.com/1422-0067/21/17/6060 |
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