Structure-guided modification of Rhizomucor miehei lipase for production of structured lipids.

Structure-guided modification of Rhizomucor miehei lipase for production of structured lipids.

To improve the performance of yeast surface-displayed Rhizomucor miehei lipase (RML) in the production of human milk fat substitute (HMFS), we mutated amino acids in the lipase substrate-binding pocket based on protein hydrophobicity, to improve esterification activity. Five mutants: Asn87Ile, Asn87...

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Bibliographic Details
Main Authors: Jun-Hui Zhang, Yu-Yan Jiang, Ying Lin, Yu-Fei Sun, Sui-Ping Zheng, Shuang-Yan Han
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3700896?pdf=render

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