Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons
Abstract Caldendrin is a Ca2+ binding protein that interacts with multiple effectors, such as the Cav1 L-type Ca2+ channel, which play a prominent role in regulating the outgrowth of dendrites and axons (i.e., neurites) during development and in response to injury. Here, we investigated the role of...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-29622-9 |
| _version_ | 1797864610670313472 |
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| author | Josue A. Lopez Annamarie Yamamoto Joseph T. Vecchi Jussara Hagen Kyungmoo Lee Milan Sonka Marlan R. Hansen Amy Lee |
| author_facet | Josue A. Lopez Annamarie Yamamoto Joseph T. Vecchi Jussara Hagen Kyungmoo Lee Milan Sonka Marlan R. Hansen Amy Lee |
| author_sort | Josue A. Lopez |
| collection | DOAJ |
| description | Abstract Caldendrin is a Ca2+ binding protein that interacts with multiple effectors, such as the Cav1 L-type Ca2+ channel, which play a prominent role in regulating the outgrowth of dendrites and axons (i.e., neurites) during development and in response to injury. Here, we investigated the role of caldendrin in Cav1-dependent pathways that impinge upon neurite growth in dorsal root ganglion neurons (DRGNs). By immunofluorescence, caldendrin was localized in medium- and large- diameter DRGNs. Compared to DRGNs cultured from WT mice, DRGNs of caldendrin knockout (KO) mice exhibited enhanced neurite regeneration and outgrowth. Strong depolarization, which normally represses neurite growth through activation of Cav1 channels, had no effect on neurite growth in DRGN cultures from female caldendrin KO mice. Remarkably, DRGNs from caldendrin KO males were no different from those of WT males in terms of depolarization-dependent neurite growth repression. We conclude that caldendrin opposes neurite regeneration and growth, and this involves coupling of Cav1 channels to growth-inhibitory pathways in DRGNs of females but not males. |
| first_indexed | 2024-04-09T22:55:49Z |
| format | Article |
| id | doaj.art-21d2febb5c354dc8aa9334ab210b832e |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T22:55:49Z |
| publishDate | 2023-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-21d2febb5c354dc8aa9334ab210b832e2023-03-22T11:16:21ZengNature PortfolioScientific Reports2045-23222023-02-0113111310.1038/s41598-023-29622-9Caldendrin represses neurite regeneration and growth in dorsal root ganglion neuronsJosue A. Lopez0Annamarie Yamamoto1Joseph T. Vecchi2Jussara Hagen3Kyungmoo Lee4Milan Sonka5Marlan R. Hansen6Amy Lee7Department of Neuroscience, University of Texas-AustinDepartment of Neuroscience, University of Texas-AustinDepartment of Molecular Physiology and Biophysics and Otolaryngology Head-Neck Surgery, Iowa Neuroscience Institute, Pappajohn Biomedical Institute, University of IowaDepartment of Molecular Physiology and Biophysics and Otolaryngology Head-Neck Surgery, Iowa Neuroscience Institute, Pappajohn Biomedical Institute, University of IowaElectrical and Computer Engineering, Iowa Institute for Biomedical Imaging, University of IowaElectrical and Computer Engineering, Iowa Institute for Biomedical Imaging, University of IowaDepartment of Molecular Physiology and Biophysics and Otolaryngology Head-Neck Surgery, Iowa Neuroscience Institute, Pappajohn Biomedical Institute, University of IowaDepartment of Neuroscience, University of Texas-AustinAbstract Caldendrin is a Ca2+ binding protein that interacts with multiple effectors, such as the Cav1 L-type Ca2+ channel, which play a prominent role in regulating the outgrowth of dendrites and axons (i.e., neurites) during development and in response to injury. Here, we investigated the role of caldendrin in Cav1-dependent pathways that impinge upon neurite growth in dorsal root ganglion neurons (DRGNs). By immunofluorescence, caldendrin was localized in medium- and large- diameter DRGNs. Compared to DRGNs cultured from WT mice, DRGNs of caldendrin knockout (KO) mice exhibited enhanced neurite regeneration and outgrowth. Strong depolarization, which normally represses neurite growth through activation of Cav1 channels, had no effect on neurite growth in DRGN cultures from female caldendrin KO mice. Remarkably, DRGNs from caldendrin KO males were no different from those of WT males in terms of depolarization-dependent neurite growth repression. We conclude that caldendrin opposes neurite regeneration and growth, and this involves coupling of Cav1 channels to growth-inhibitory pathways in DRGNs of females but not males.https://doi.org/10.1038/s41598-023-29622-9 |
| spellingShingle | Josue A. Lopez Annamarie Yamamoto Joseph T. Vecchi Jussara Hagen Kyungmoo Lee Milan Sonka Marlan R. Hansen Amy Lee Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons Scientific Reports |
| title | Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons |
| title_full | Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons |
| title_fullStr | Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons |
| title_full_unstemmed | Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons |
| title_short | Caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons |
| title_sort | caldendrin represses neurite regeneration and growth in dorsal root ganglion neurons |
| url | https://doi.org/10.1038/s41598-023-29622-9 |
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