Cloning and Expression of Human Norovirus GI.5 and GII.4 P Proteins and Their Binding Characteristics with Histo-Blood Group Antigens-like Substances in Pacific Oysters
To clarify the binding characteristics of human norovirus (HuNoV) with histo-blood group antigens (HBGAs)-like substances from Pacific oysters, this study used an Escherichia coli expression system to clone and express HuNoV GI.5 and GII.4 P proteins, and analyzed the binding characteristics of HuNo...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
China Food Publishing Company
2024-01-01
|
| Series: | Shipin Kexue |
| Subjects: | |
| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-2-015.pdf |
| _version_ | 1827347452253962240 |
|---|---|
| author | TONG Lihui, YANG Min, WANG Shanshan, WANG Dajun, WANG Mingli, ZHOU Deqing |
| author_facet | TONG Lihui, YANG Min, WANG Shanshan, WANG Dajun, WANG Mingli, ZHOU Deqing |
| author_sort | TONG Lihui, YANG Min, WANG Shanshan, WANG Dajun, WANG Mingli, ZHOU Deqing |
| collection | DOAJ |
| description | To clarify the binding characteristics of human norovirus (HuNoV) with histo-blood group antigens (HBGAs)-like substances from Pacific oysters, this study used an Escherichia coli expression system to clone and express HuNoV GI.5 and GII.4 P proteins, and analyzed the binding characteristics of HuNoV P proteins with salivary HBGAs and HBGAs-like substances from Pacific oysters using enzyme-linked immunosorbent assay. The results showed that HuNoV GII.4 exhibited good binding characteristics with blood type A, B, AB, and O salivary HBGAs, while GI.5 HuNoV exhibited weak binding characteristics with blood type B salivary HBGAs but had significant advantage in binding with type O salivary HBGAs. HuNoV GI.5 and GII.4 could be bioaccumulated in the gills, digestive gland, and mantle of Pacific oysters, with the highest bioaccumulation in the digestive gland. Both types of HuNoV were mainly bound to type A and H1 HBGAs-like substances; HuNoV GII.4 had different degrees of binding with type Lea, Leb, Lex, and Ley HBGAs-like substances, while HuNoV GI.5 had weak binding with type Leb HBGAs-like substances but significant advantage in binding with type H1 HBGAs-like substances. In summary, different types of HuNoV have different binding characteristics with HBGAs or HBGAs-like substances. Specifically, HuNoV GII.4 shows broad-spectrum binding characteristics whereas HuNoV GI.5 shows selective binding characteristics. |
| first_indexed | 2024-03-07T23:50:48Z |
| format | Article |
| id | doaj.art-21d594a0fe0048279b7e2294ecfd850d |
| institution | Directory Open Access Journal |
| issn | 1002-6630 |
| language | English |
| last_indexed | 2024-03-07T23:50:48Z |
| publishDate | 2024-01-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj.art-21d594a0fe0048279b7e2294ecfd850d2024-02-19T05:50:22ZengChina Food Publishing CompanyShipin Kexue1002-66302024-01-0145211311910.7506/spkx1002-6630-20230329-284Cloning and Expression of Human Norovirus GI.5 and GII.4 P Proteins and Their Binding Characteristics with Histo-Blood Group Antigens-like Substances in Pacific OystersTONG Lihui, YANG Min, WANG Shanshan, WANG Dajun, WANG Mingli, ZHOU Deqing0(1. Yellow China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Laboratory of Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology, Qingdao 266071, China; 2. Qingdao Binhai University, Qingdao 266555, China; 3. Yantai Haiyu Food Co. Ltd., Yantai 264000, China; 4. Penglai Huiyang Food Co. Ltd., Yantai 264000, China)To clarify the binding characteristics of human norovirus (HuNoV) with histo-blood group antigens (HBGAs)-like substances from Pacific oysters, this study used an Escherichia coli expression system to clone and express HuNoV GI.5 and GII.4 P proteins, and analyzed the binding characteristics of HuNoV P proteins with salivary HBGAs and HBGAs-like substances from Pacific oysters using enzyme-linked immunosorbent assay. The results showed that HuNoV GII.4 exhibited good binding characteristics with blood type A, B, AB, and O salivary HBGAs, while GI.5 HuNoV exhibited weak binding characteristics with blood type B salivary HBGAs but had significant advantage in binding with type O salivary HBGAs. HuNoV GI.5 and GII.4 could be bioaccumulated in the gills, digestive gland, and mantle of Pacific oysters, with the highest bioaccumulation in the digestive gland. Both types of HuNoV were mainly bound to type A and H1 HBGAs-like substances; HuNoV GII.4 had different degrees of binding with type Lea, Leb, Lex, and Ley HBGAs-like substances, while HuNoV GI.5 had weak binding with type Leb HBGAs-like substances but significant advantage in binding with type H1 HBGAs-like substances. In summary, different types of HuNoV have different binding characteristics with HBGAs or HBGAs-like substances. Specifically, HuNoV GII.4 shows broad-spectrum binding characteristics whereas HuNoV GI.5 shows selective binding characteristics.https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-2-015.pdfhuman norovirus; p protein; pacific oyster; histo-blood group antigens; binding characteristics |
| spellingShingle | TONG Lihui, YANG Min, WANG Shanshan, WANG Dajun, WANG Mingli, ZHOU Deqing Cloning and Expression of Human Norovirus GI.5 and GII.4 P Proteins and Their Binding Characteristics with Histo-Blood Group Antigens-like Substances in Pacific Oysters Shipin Kexue human norovirus; p protein; pacific oyster; histo-blood group antigens; binding characteristics |
| title | Cloning and Expression of Human Norovirus GI.5 and GII.4 P Proteins and Their Binding Characteristics with Histo-Blood Group Antigens-like Substances in Pacific Oysters |
| title_full | Cloning and Expression of Human Norovirus GI.5 and GII.4 P Proteins and Their Binding Characteristics with Histo-Blood Group Antigens-like Substances in Pacific Oysters |
| title_fullStr | Cloning and Expression of Human Norovirus GI.5 and GII.4 P Proteins and Their Binding Characteristics with Histo-Blood Group Antigens-like Substances in Pacific Oysters |
| title_full_unstemmed | Cloning and Expression of Human Norovirus GI.5 and GII.4 P Proteins and Their Binding Characteristics with Histo-Blood Group Antigens-like Substances in Pacific Oysters |
| title_short | Cloning and Expression of Human Norovirus GI.5 and GII.4 P Proteins and Their Binding Characteristics with Histo-Blood Group Antigens-like Substances in Pacific Oysters |
| title_sort | cloning and expression of human norovirus gi 5 and gii 4 p proteins and their binding characteristics with histo blood group antigens like substances in pacific oysters |
| topic | human norovirus; p protein; pacific oyster; histo-blood group antigens; binding characteristics |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-2-015.pdf |
| work_keys_str_mv | AT tonglihuiyangminwangshanshanwangdajunwangminglizhoudeqing cloningandexpressionofhumannorovirusgi5andgii4pproteinsandtheirbindingcharacteristicswithhistobloodgroupantigenslikesubstancesinpacificoysters |