Cloning and Characterization of Sf9 Cell Lamin and the Lamin Conformational Changes during Autographa californica multiple nucleopolyhedrovirus Infection
At present, the details of lamina alterations after baculovirus infection remain elusive. In this study, a lamin gene in the Sf9 cell line of Spodoptera frugiperda was cloned. The open reading frame (orf) of the Sf9 lamin was 1860 bp and encoded a protein with a molecular weight of 70 kDa. A transfe...
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MDPI AG
2016-05-01
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| Series: | Viruses |
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| Online Access: | http://www.mdpi.com/1999-4915/8/5/126 |
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| author | Wenqiang Wei Hongju Wang Xiaoya Li Na Fang Shili Yang Hongyan Liu Xiaonan Kang Xiulian Sun Shaoping Ji |
| author_facet | Wenqiang Wei Hongju Wang Xiaoya Li Na Fang Shili Yang Hongyan Liu Xiaonan Kang Xiulian Sun Shaoping Ji |
| author_sort | Wenqiang Wei |
| collection | DOAJ |
| description | At present, the details of lamina alterations after baculovirus infection remain elusive. In this study, a lamin gene in the Sf9 cell line of Spodoptera frugiperda was cloned. The open reading frame (orf) of the Sf9 lamin was 1860 bp and encoded a protein with a molecular weight of 70 kDa. A transfection assay with a red fluorescence protein (rfp)-lamin fusion protein indicated that Sf9 lamin was localized in the nuclear rim. Transmission electron microscopy observations indicated that Autographa californica multiple nucleopolyhedrovirus (AcMNPV) nucleocapsids may pass through the nuclear envelope. Immunofluorescence assay indicated that the lamina showed a ruffled staining pattern with the formation of invaginations in the Sf9 cells infected with AcMNPV, while it was evenly distributed at the nuclear periphery of mock-infected cells. Western blotting results indicated that the total amount of lamin in the baculovirus-infected Sf9 cells was significantly decreased compared with the mock-infected cells. These results imply that AcMNPV infection induces structural and biochemical rearrangements of lamina of Sf9 cells. |
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| id | doaj.art-21da651cdce74828b3e5082f262e0f8f |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-20T19:43:17Z |
| publishDate | 2016-05-01 |
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| series | Viruses |
| spelling | doaj.art-21da651cdce74828b3e5082f262e0f8f2022-12-21T19:28:29ZengMDPI AGViruses1999-49152016-05-018512610.3390/v8050126v8050126Cloning and Characterization of Sf9 Cell Lamin and the Lamin Conformational Changes during Autographa californica multiple nucleopolyhedrovirus InfectionWenqiang Wei0Hongju Wang1Xiaoya Li2Na Fang3Shili Yang4Hongyan Liu5Xiaonan Kang6Xiulian Sun7Shaoping Ji8Laboratory of Cell Signal Transduction, Medical School, Henan University, Kaifeng 475004, ChinaLaboratory of Cell Signal Transduction, Medical School, Henan University, Kaifeng 475004, ChinaSchool of Education Science, Henan University, Kaifeng 475004, ChinaLaboratory of Cell Signal Transduction, Medical School, Henan University, Kaifeng 475004, ChinaKey Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, ChinaLaboratory of Cell Signal Transduction, Medical School, Henan University, Kaifeng 475004, ChinaSchool of Education Science, Henan University, Kaifeng 475004, ChinaKey Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, ChinaLaboratory of Cell Signal Transduction, Medical School, Henan University, Kaifeng 475004, ChinaAt present, the details of lamina alterations after baculovirus infection remain elusive. In this study, a lamin gene in the Sf9 cell line of Spodoptera frugiperda was cloned. The open reading frame (orf) of the Sf9 lamin was 1860 bp and encoded a protein with a molecular weight of 70 kDa. A transfection assay with a red fluorescence protein (rfp)-lamin fusion protein indicated that Sf9 lamin was localized in the nuclear rim. Transmission electron microscopy observations indicated that Autographa californica multiple nucleopolyhedrovirus (AcMNPV) nucleocapsids may pass through the nuclear envelope. Immunofluorescence assay indicated that the lamina showed a ruffled staining pattern with the formation of invaginations in the Sf9 cells infected with AcMNPV, while it was evenly distributed at the nuclear periphery of mock-infected cells. Western blotting results indicated that the total amount of lamin in the baculovirus-infected Sf9 cells was significantly decreased compared with the mock-infected cells. These results imply that AcMNPV infection induces structural and biochemical rearrangements of lamina of Sf9 cells.http://www.mdpi.com/1999-4915/8/5/126Autographa californica multiple nucleopolyhedrovirusinfectionSf9 cellslamina |
| spellingShingle | Wenqiang Wei Hongju Wang Xiaoya Li Na Fang Shili Yang Hongyan Liu Xiaonan Kang Xiulian Sun Shaoping Ji Cloning and Characterization of Sf9 Cell Lamin and the Lamin Conformational Changes during Autographa californica multiple nucleopolyhedrovirus Infection Viruses Autographa californica multiple nucleopolyhedrovirus infection Sf9 cells lamina |
| title | Cloning and Characterization of Sf9 Cell Lamin and the Lamin Conformational Changes during Autographa californica multiple nucleopolyhedrovirus Infection |
| title_full | Cloning and Characterization of Sf9 Cell Lamin and the Lamin Conformational Changes during Autographa californica multiple nucleopolyhedrovirus Infection |
| title_fullStr | Cloning and Characterization of Sf9 Cell Lamin and the Lamin Conformational Changes during Autographa californica multiple nucleopolyhedrovirus Infection |
| title_full_unstemmed | Cloning and Characterization of Sf9 Cell Lamin and the Lamin Conformational Changes during Autographa californica multiple nucleopolyhedrovirus Infection |
| title_short | Cloning and Characterization of Sf9 Cell Lamin and the Lamin Conformational Changes during Autographa californica multiple nucleopolyhedrovirus Infection |
| title_sort | cloning and characterization of sf9 cell lamin and the lamin conformational changes during autographa californica multiple nucleopolyhedrovirus infection |
| topic | Autographa californica multiple nucleopolyhedrovirus infection Sf9 cells lamina |
| url | http://www.mdpi.com/1999-4915/8/5/126 |
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