A network-based analysis of key pharmacological pathways of Corilagin acting on Breast cancer and experimental validation
The combination of network pharmacological methods and experimental verification has revealed the underlying molecular mechanism of Corilagin in the treatment of breast cancer. In this study, we used Swiss Target Prediction database network to predict the possible candidate targets and pathways of C...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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EDP Sciences
2020-01-01
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| Series: | E3S Web of Conferences |
| Online Access: | https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/45/e3sconf_iceeb2020_02004.pdf |
| _version_ | 1818570243981705216 |
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| author | Zhong Xue Tang Jintian Long Wenyue Cai Xu Wang Huiguo Li Qian |
| author_facet | Zhong Xue Tang Jintian Long Wenyue Cai Xu Wang Huiguo Li Qian |
| author_sort | Zhong Xue |
| collection | DOAJ |
| description | The combination of network pharmacological methods and experimental verification has revealed the underlying molecular mechanism of Corilagin in the treatment of breast cancer. In this study, we used Swiss Target Prediction database network to predict the possible candidate targets and pathways of Coilagin, and collect breast cancer targets and pathways of CooLGeN. The result of network pharmacological screen indicated that Bcl is a key pathway for Corilagin in the treatment of breast cancer. The CCK-8 experiment also proved that Corilagin could inhibit the proliferation of MCF-7 cells. Western Blot was used for the validation of network pharmacological results, which showed that Corilagin could increase the apoptosis rates (Bcl-2/Bax) of MCF-7 cells, and up-regulates the apoptotic protein Caspase-3 and changes the Bcl-2 family proteins expression in MCF-7 cells. All results suggest that Corilagin has the potential therapeutic effects on breast cancer, and may be useful for adjuvant chemotherapy. |
| first_indexed | 2024-12-14T06:58:07Z |
| format | Article |
| id | doaj.art-21dc0823d607478aa21c610d023948e4 |
| institution | Directory Open Access Journal |
| issn | 2267-1242 |
| language | English |
| last_indexed | 2024-12-14T06:58:07Z |
| publishDate | 2020-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | E3S Web of Conferences |
| spelling | doaj.art-21dc0823d607478aa21c610d023948e42022-12-21T23:12:34ZengEDP SciencesE3S Web of Conferences2267-12422020-01-011850200410.1051/e3sconf/202018502004e3sconf_iceeb2020_02004A network-based analysis of key pharmacological pathways of Corilagin acting on Breast cancer and experimental validationZhong Xue0Tang Jintian1Long Wenyue2Cai Xu3Wang Huiguo4Li Qian5College of Life Science and Technology, Dalian UniversityDepartment of Engineering Physics, Institute of Medical physics, Tsinghua UniversityCollege of Life Science and Technology, Dalian UniversityDepartment of Engineering Physics, Institute of Medical physics, Tsinghua UniversityCollege of Life Science and Technology, Dalian UniversityDepartment of Engineering Physics, Institute of Medical physics, Tsinghua UniversityThe combination of network pharmacological methods and experimental verification has revealed the underlying molecular mechanism of Corilagin in the treatment of breast cancer. In this study, we used Swiss Target Prediction database network to predict the possible candidate targets and pathways of Coilagin, and collect breast cancer targets and pathways of CooLGeN. The result of network pharmacological screen indicated that Bcl is a key pathway for Corilagin in the treatment of breast cancer. The CCK-8 experiment also proved that Corilagin could inhibit the proliferation of MCF-7 cells. Western Blot was used for the validation of network pharmacological results, which showed that Corilagin could increase the apoptosis rates (Bcl-2/Bax) of MCF-7 cells, and up-regulates the apoptotic protein Caspase-3 and changes the Bcl-2 family proteins expression in MCF-7 cells. All results suggest that Corilagin has the potential therapeutic effects on breast cancer, and may be useful for adjuvant chemotherapy.https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/45/e3sconf_iceeb2020_02004.pdf |
| spellingShingle | Zhong Xue Tang Jintian Long Wenyue Cai Xu Wang Huiguo Li Qian A network-based analysis of key pharmacological pathways of Corilagin acting on Breast cancer and experimental validation E3S Web of Conferences |
| title | A network-based analysis of key pharmacological pathways of Corilagin acting on Breast cancer and experimental validation |
| title_full | A network-based analysis of key pharmacological pathways of Corilagin acting on Breast cancer and experimental validation |
| title_fullStr | A network-based analysis of key pharmacological pathways of Corilagin acting on Breast cancer and experimental validation |
| title_full_unstemmed | A network-based analysis of key pharmacological pathways of Corilagin acting on Breast cancer and experimental validation |
| title_short | A network-based analysis of key pharmacological pathways of Corilagin acting on Breast cancer and experimental validation |
| title_sort | network based analysis of key pharmacological pathways of corilagin acting on breast cancer and experimental validation |
| url | https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/45/e3sconf_iceeb2020_02004.pdf |
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