Using Genipin to Immobilize Bone Morphogenetic Protein-2 on Zirconia Surface for Enhancing Cell Adhesion and Mineralization in Dental Implant Applications
Our objective in this study was to promote cell responses through the immobilization of bone morphogenetic protein-2 (BMP-2) on roughened zirconia (ZrO<sub>2</sub>) through using the natural cross-linker genipin in dental implant applications. Field emission scanning electron microscope,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/12/11/2639 |
| _version_ | 1797548315175288832 |
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| author | Ying-Sui Sun Yu-An Lin Her-Hsiung Huang |
| author_facet | Ying-Sui Sun Yu-An Lin Her-Hsiung Huang |
| author_sort | Ying-Sui Sun |
| collection | DOAJ |
| description | Our objective in this study was to promote cell responses through the immobilization of bone morphogenetic protein-2 (BMP-2) on roughened zirconia (ZrO<sub>2</sub>) through using the natural cross-linker genipin in dental implant applications. Field emission scanning electron microscope, X-ray photoelectron spectroscopy, and attenuated total reflection-Fourier transform infrared spectroscopy were used to analyze the surface characterizations, including the topography, chemistry, and functional groups, respectively, of the test specimens. Human bone marrow mesenchymal stem cells (hMSCs) were used to detect cell responses (adhesion, proliferation, and mineralization). The surface characterizations analysis results revealed that genipin was effective in immobilizing BMP-2 on roughened zirconia surfaces. BMP-2 proved effective in promoting the adhesion and mineralization of hMSCs on roughened zirconia. The surface modification proposed has potential in zirconia dental implant applications. |
| first_indexed | 2024-03-10T14:57:36Z |
| format | Article |
| id | doaj.art-21e041050c1541a4aabd69b24bee4bec |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T14:57:36Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-21e041050c1541a4aabd69b24bee4bec2023-11-20T20:26:24ZengMDPI AGPolymers2073-43602020-11-011211263910.3390/polym12112639Using Genipin to Immobilize Bone Morphogenetic Protein-2 on Zirconia Surface for Enhancing Cell Adhesion and Mineralization in Dental Implant ApplicationsYing-Sui Sun0Yu-An Lin1Her-Hsiung Huang2School of Dental Technology, Taipei Medical University, Taipei 11031, TaiwanInstitute of Oral Biology, National Yang-Ming University, Taipei 11221, TaiwanDepartment of Dentistry, National Yang-Ming University, Taipei 11221, TaiwanOur objective in this study was to promote cell responses through the immobilization of bone morphogenetic protein-2 (BMP-2) on roughened zirconia (ZrO<sub>2</sub>) through using the natural cross-linker genipin in dental implant applications. Field emission scanning electron microscope, X-ray photoelectron spectroscopy, and attenuated total reflection-Fourier transform infrared spectroscopy were used to analyze the surface characterizations, including the topography, chemistry, and functional groups, respectively, of the test specimens. Human bone marrow mesenchymal stem cells (hMSCs) were used to detect cell responses (adhesion, proliferation, and mineralization). The surface characterizations analysis results revealed that genipin was effective in immobilizing BMP-2 on roughened zirconia surfaces. BMP-2 proved effective in promoting the adhesion and mineralization of hMSCs on roughened zirconia. The surface modification proposed has potential in zirconia dental implant applications.https://www.mdpi.com/2073-4360/12/11/2639natural cross-linker genipinbone morphogenetic protein-2 (BMP-2)human bone marrow mesenchymal stem cellcell adhesioncell mineralization |
| spellingShingle | Ying-Sui Sun Yu-An Lin Her-Hsiung Huang Using Genipin to Immobilize Bone Morphogenetic Protein-2 on Zirconia Surface for Enhancing Cell Adhesion and Mineralization in Dental Implant Applications Polymers natural cross-linker genipin bone morphogenetic protein-2 (BMP-2) human bone marrow mesenchymal stem cell cell adhesion cell mineralization |
| title | Using Genipin to Immobilize Bone Morphogenetic Protein-2 on Zirconia Surface for Enhancing Cell Adhesion and Mineralization in Dental Implant Applications |
| title_full | Using Genipin to Immobilize Bone Morphogenetic Protein-2 on Zirconia Surface for Enhancing Cell Adhesion and Mineralization in Dental Implant Applications |
| title_fullStr | Using Genipin to Immobilize Bone Morphogenetic Protein-2 on Zirconia Surface for Enhancing Cell Adhesion and Mineralization in Dental Implant Applications |
| title_full_unstemmed | Using Genipin to Immobilize Bone Morphogenetic Protein-2 on Zirconia Surface for Enhancing Cell Adhesion and Mineralization in Dental Implant Applications |
| title_short | Using Genipin to Immobilize Bone Morphogenetic Protein-2 on Zirconia Surface for Enhancing Cell Adhesion and Mineralization in Dental Implant Applications |
| title_sort | using genipin to immobilize bone morphogenetic protein 2 on zirconia surface for enhancing cell adhesion and mineralization in dental implant applications |
| topic | natural cross-linker genipin bone morphogenetic protein-2 (BMP-2) human bone marrow mesenchymal stem cell cell adhesion cell mineralization |
| url | https://www.mdpi.com/2073-4360/12/11/2639 |
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