Direct blood fluorescence signal intensity of neutrophils (NEU-SFL): A predictive marker of death in hospitalized COVID-19 patients?
IntroductionCoronavirus disease 2019 (COVID-19) is a respiratory disease triggered by immunopathological mechanisms that cause excessive inflammation and leukocyte dysfunction. Neutrophils play a critical role in the innate immunity and are able to produce neutrophil extracellular traps (NETs: NETos...
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| Format: | Article |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2022.1062112/full |
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| author | Mathieu Fortier Mathias Chea Charlène Aïn Maxime Loyens Thierry Boudemaghe Thierry Boudemaghe Jean-Christophe Gris Jean-Christophe Gris Jean-Christophe Gris Jean-Christophe Gris Sylvie Bouvier Sylvie Bouvier Sylvie Bouvier |
| author_facet | Mathieu Fortier Mathias Chea Charlène Aïn Maxime Loyens Thierry Boudemaghe Thierry Boudemaghe Jean-Christophe Gris Jean-Christophe Gris Jean-Christophe Gris Jean-Christophe Gris Sylvie Bouvier Sylvie Bouvier Sylvie Bouvier |
| author_sort | Mathieu Fortier |
| collection | DOAJ |
| description | IntroductionCoronavirus disease 2019 (COVID-19) is a respiratory disease triggered by immunopathological mechanisms that cause excessive inflammation and leukocyte dysfunction. Neutrophils play a critical role in the innate immunity and are able to produce neutrophil extracellular traps (NETs: NETosis process) to combat infections. Some NETs markers are increased in patients who died from COVID-19. Recently, the neutrophil fluorescence variable (NEU-SFL), available on certain automated complete blood count (CBC) analyzers, has been correlated with NET formation and may reflect NETosis in patients. Here we evaluate whether NEU-SFL measured after admission of COVID-19 patients is associated with in-hospital survival or death.Patients and methods1,852 patients admitted for severe COVID-19 at Nîmes University Hospital in 2021 were retrospectively included in the study: 1,564 who survived the hospital stay and 288 who did not. The NEU-SFL was obtained on the Sysmex™ XN-10® analyzer and values for survivors and non-survivors were compared. The intra-patient NEU-SFL variations between the hospital entry and the last day of hospitalization were also analyzed (IRB 22.06.01, NCT 05413824).ResultsNon-survivors presented higher NEU-SFL values. NEU-SFL values above the 4th quartile were independently associated with a 2.88-fold risk of death. Furthermore, the difference of NEU-SFL values between the first and the last available data during hospitalization revealed that a decrease in NEU-SFL was associated to survivors and vice versa.ConclusionOur study reinforces the role of neutrophils and NETosis in the pathophysiology and prognosis of COVID-19. Further studies combining NEU-SFL with other NETosis markers could improve the management of COVID-19 patients. |
| first_indexed | 2024-04-13T03:43:17Z |
| format | Article |
| id | doaj.art-21e4581aa5234d859081cb2fe8488cea |
| institution | Directory Open Access Journal |
| issn | 2296-858X |
| language | English |
| last_indexed | 2024-04-13T03:43:17Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Medicine |
| spelling | doaj.art-21e4581aa5234d859081cb2fe8488cea2022-12-22T03:04:05ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2022-12-01910.3389/fmed.2022.10621121062112Direct blood fluorescence signal intensity of neutrophils (NEU-SFL): A predictive marker of death in hospitalized COVID-19 patients?Mathieu Fortier0Mathias Chea1Charlène Aïn2Maxime Loyens3Thierry Boudemaghe4Thierry Boudemaghe5Jean-Christophe Gris6Jean-Christophe Gris7Jean-Christophe Gris8Jean-Christophe Gris9Sylvie Bouvier10Sylvie Bouvier11Sylvie Bouvier12Department of Hematology, University Hospital, Nîmes, FranceUA11 INSERM – UM Institut Desbrest d’Épidémiologie et de Santé Publique (IDESP), Montpellier, FranceDepartment of Hematology, University Hospital, Nîmes, FranceDepartment of Hematology, University Hospital, Nîmes, FranceUA11 INSERM – UM Institut Desbrest d’Épidémiologie et de Santé Publique (IDESP), Montpellier, FranceDepartment of Medical Information, Methods and Research, Centre Hospitalier Universitaire de Nîmes, University of Montpellier, Nîmes, FranceDepartment of Hematology, University Hospital, Nîmes, FranceUA11 INSERM – UM Institut Desbrest d’Épidémiologie et de Santé Publique (IDESP), Montpellier, FranceI.M. Sechenov First Moscow State Medical University, Moscow, RussiaFaculty of Pharmaceutical and Biological Sciences, Montpellier University, Montpellier, FranceDepartment of Hematology, University Hospital, Nîmes, FranceUA11 INSERM – UM Institut Desbrest d’Épidémiologie et de Santé Publique (IDESP), Montpellier, FranceFaculty of Pharmaceutical and Biological Sciences, Montpellier University, Montpellier, FranceIntroductionCoronavirus disease 2019 (COVID-19) is a respiratory disease triggered by immunopathological mechanisms that cause excessive inflammation and leukocyte dysfunction. Neutrophils play a critical role in the innate immunity and are able to produce neutrophil extracellular traps (NETs: NETosis process) to combat infections. Some NETs markers are increased in patients who died from COVID-19. Recently, the neutrophil fluorescence variable (NEU-SFL), available on certain automated complete blood count (CBC) analyzers, has been correlated with NET formation and may reflect NETosis in patients. Here we evaluate whether NEU-SFL measured after admission of COVID-19 patients is associated with in-hospital survival or death.Patients and methods1,852 patients admitted for severe COVID-19 at Nîmes University Hospital in 2021 were retrospectively included in the study: 1,564 who survived the hospital stay and 288 who did not. The NEU-SFL was obtained on the Sysmex™ XN-10® analyzer and values for survivors and non-survivors were compared. The intra-patient NEU-SFL variations between the hospital entry and the last day of hospitalization were also analyzed (IRB 22.06.01, NCT 05413824).ResultsNon-survivors presented higher NEU-SFL values. NEU-SFL values above the 4th quartile were independently associated with a 2.88-fold risk of death. Furthermore, the difference of NEU-SFL values between the first and the last available data during hospitalization revealed that a decrease in NEU-SFL was associated to survivors and vice versa.ConclusionOur study reinforces the role of neutrophils and NETosis in the pathophysiology and prognosis of COVID-19. Further studies combining NEU-SFL with other NETosis markers could improve the management of COVID-19 patients.https://www.frontiersin.org/articles/10.3389/fmed.2022.1062112/fullCOVID-19fluorescence signal intensityimmunothrombosisNETosisneutrophil |
| spellingShingle | Mathieu Fortier Mathias Chea Charlène Aïn Maxime Loyens Thierry Boudemaghe Thierry Boudemaghe Jean-Christophe Gris Jean-Christophe Gris Jean-Christophe Gris Jean-Christophe Gris Sylvie Bouvier Sylvie Bouvier Sylvie Bouvier Direct blood fluorescence signal intensity of neutrophils (NEU-SFL): A predictive marker of death in hospitalized COVID-19 patients? Frontiers in Medicine COVID-19 fluorescence signal intensity immunothrombosis NETosis neutrophil |
| title | Direct blood fluorescence signal intensity of neutrophils (NEU-SFL): A predictive marker of death in hospitalized COVID-19 patients? |
| title_full | Direct blood fluorescence signal intensity of neutrophils (NEU-SFL): A predictive marker of death in hospitalized COVID-19 patients? |
| title_fullStr | Direct blood fluorescence signal intensity of neutrophils (NEU-SFL): A predictive marker of death in hospitalized COVID-19 patients? |
| title_full_unstemmed | Direct blood fluorescence signal intensity of neutrophils (NEU-SFL): A predictive marker of death in hospitalized COVID-19 patients? |
| title_short | Direct blood fluorescence signal intensity of neutrophils (NEU-SFL): A predictive marker of death in hospitalized COVID-19 patients? |
| title_sort | direct blood fluorescence signal intensity of neutrophils neu sfl a predictive marker of death in hospitalized covid 19 patients |
| topic | COVID-19 fluorescence signal intensity immunothrombosis NETosis neutrophil |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2022.1062112/full |
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