Temporal Expression Profiles Reveal Potential Targets during Postembryonic Development of Forensically Important <i>Sarcophaga peregrina</i> (Diptera: Sarcophagidae)
<i>Sarcophaga peregrina</i> (Robineau-Desvoidy, 1830) is a species of medical and forensic importance. In order to investigate the molecular mechanism during postembryonic development and identify specific genes that may serve as potential targets, transcriptome analysis was used to inve...
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MDPI AG
2022-05-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/13/5/453 |
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| author | Lipin Ren Yanjie Shang Xiangyan Zhang Shan Chen Yunna Zheng Ying Zou Yihong Qu Jifeng Cai Changquan Zhang Yadong Guo |
| author_facet | Lipin Ren Yanjie Shang Xiangyan Zhang Shan Chen Yunna Zheng Ying Zou Yihong Qu Jifeng Cai Changquan Zhang Yadong Guo |
| author_sort | Lipin Ren |
| collection | DOAJ |
| description | <i>Sarcophaga peregrina</i> (Robineau-Desvoidy, 1830) is a species of medical and forensic importance. In order to investigate the molecular mechanism during postembryonic development and identify specific genes that may serve as potential targets, transcriptome analysis was used to investigate its gene expression dynamics from the larval to pupal stages, based on our previous <i>de novo</i>-assembled genome of <i>S. peregrina</i>. Totals of 2457, 3656, 3764, and 2554 differentially expressed genes were identified. The specific genes encoding the structural constituent of cuticle were significantly differentially expressed, suggesting that degradation and synthesis of cuticle-related proteins might actively occur during metamorphosis. Molting (20-hydroxyecdysone, 20E) and juvenile (JH) hormone pathways were significantly enriched, and gene expression levels changed in a dynamic pattern during the developmental stages. In addition, the genes in the oxidative phosphorylation pathway were significantly expressed at a high level during the larval stage, and down-regulated from the wandering to pupal stages. Weighted gene co-expression correlation network analysis (WGCNA) further demonstrated the potential regulation mechanism of tyrosine metabolism in the process of puparium tanning. Moreover, 10 consistently up-regulated genes were further validated by qRT-PCR. The utility of the models was then examined in a blind study, indicating the ability to predict larval development. The developmental, stage-specific gene profiles suggest novel molecular markers for age prediction of forensically important flies. |
| first_indexed | 2024-03-10T03:41:14Z |
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| id | doaj.art-21e522b4998e423ab9efc5b9e7da1561 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T03:41:14Z |
| publishDate | 2022-05-01 |
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| series | Insects |
| spelling | doaj.art-21e522b4998e423ab9efc5b9e7da15612023-11-23T11:31:51ZengMDPI AGInsects2075-44502022-05-0113545310.3390/insects13050453Temporal Expression Profiles Reveal Potential Targets during Postembryonic Development of Forensically Important <i>Sarcophaga peregrina</i> (Diptera: Sarcophagidae)Lipin Ren0Yanjie Shang1Xiangyan Zhang2Shan Chen3Yunna Zheng4Ying Zou5Yihong Qu6Jifeng Cai7Changquan Zhang8Yadong Guo9Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha 410013, ChinaDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha 410013, ChinaDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha 410013, ChinaSchool of Ecological and Environmental Sciences, East China Normal University, Shanghai 200000, ChinaOE Biotech Co., Ltd., Shanghai 200120, ChinaDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha 410013, ChinaDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha 410013, ChinaDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha 410013, ChinaDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha 410013, ChinaDepartment of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha 410013, China<i>Sarcophaga peregrina</i> (Robineau-Desvoidy, 1830) is a species of medical and forensic importance. In order to investigate the molecular mechanism during postembryonic development and identify specific genes that may serve as potential targets, transcriptome analysis was used to investigate its gene expression dynamics from the larval to pupal stages, based on our previous <i>de novo</i>-assembled genome of <i>S. peregrina</i>. Totals of 2457, 3656, 3764, and 2554 differentially expressed genes were identified. The specific genes encoding the structural constituent of cuticle were significantly differentially expressed, suggesting that degradation and synthesis of cuticle-related proteins might actively occur during metamorphosis. Molting (20-hydroxyecdysone, 20E) and juvenile (JH) hormone pathways were significantly enriched, and gene expression levels changed in a dynamic pattern during the developmental stages. In addition, the genes in the oxidative phosphorylation pathway were significantly expressed at a high level during the larval stage, and down-regulated from the wandering to pupal stages. Weighted gene co-expression correlation network analysis (WGCNA) further demonstrated the potential regulation mechanism of tyrosine metabolism in the process of puparium tanning. Moreover, 10 consistently up-regulated genes were further validated by qRT-PCR. The utility of the models was then examined in a blind study, indicating the ability to predict larval development. The developmental, stage-specific gene profiles suggest novel molecular markers for age prediction of forensically important flies.https://www.mdpi.com/2075-4450/13/5/453<i>Sarcophaga peregrina</i>metamorphosisgene expressionage estimation |
| spellingShingle | Lipin Ren Yanjie Shang Xiangyan Zhang Shan Chen Yunna Zheng Ying Zou Yihong Qu Jifeng Cai Changquan Zhang Yadong Guo Temporal Expression Profiles Reveal Potential Targets during Postembryonic Development of Forensically Important <i>Sarcophaga peregrina</i> (Diptera: Sarcophagidae) Insects <i>Sarcophaga peregrina</i> metamorphosis gene expression age estimation |
| title | Temporal Expression Profiles Reveal Potential Targets during Postembryonic Development of Forensically Important <i>Sarcophaga peregrina</i> (Diptera: Sarcophagidae) |
| title_full | Temporal Expression Profiles Reveal Potential Targets during Postembryonic Development of Forensically Important <i>Sarcophaga peregrina</i> (Diptera: Sarcophagidae) |
| title_fullStr | Temporal Expression Profiles Reveal Potential Targets during Postembryonic Development of Forensically Important <i>Sarcophaga peregrina</i> (Diptera: Sarcophagidae) |
| title_full_unstemmed | Temporal Expression Profiles Reveal Potential Targets during Postembryonic Development of Forensically Important <i>Sarcophaga peregrina</i> (Diptera: Sarcophagidae) |
| title_short | Temporal Expression Profiles Reveal Potential Targets during Postembryonic Development of Forensically Important <i>Sarcophaga peregrina</i> (Diptera: Sarcophagidae) |
| title_sort | temporal expression profiles reveal potential targets during postembryonic development of forensically important i sarcophaga peregrina i diptera sarcophagidae |
| topic | <i>Sarcophaga peregrina</i> metamorphosis gene expression age estimation |
| url | https://www.mdpi.com/2075-4450/13/5/453 |
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