Application of Spectroscopic Methods for the Identification of Superoxide Dismutases in Cyanobacteria
Superoxide dismutases (SODs) belong to the group of metalloenzymes that remove superoxide anion radicals and they have been identified in three domains of life: <i>Bacteria</i>, <i>Archaea</i> and <i>Eucarya</i>. SODs in <i>Synechocystis</i> sp. PCC 68...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/22/13819 |
| Summary: | Superoxide dismutases (SODs) belong to the group of metalloenzymes that remove superoxide anion radicals and they have been identified in three domains of life: <i>Bacteria</i>, <i>Archaea</i> and <i>Eucarya</i>. SODs in <i>Synechocystis</i> sp. PCC 6803, <i>Gloeobacter violaceus</i> CCALA 979, and <i>Geitlerinema</i> sp. ZHR1A were investigated. We hypothesized that iron (FeSOD) and/or manganese (MnSOD) dominate as active forms in these cyanobacteria. Activity staining and three different spectroscopic methods of SOD activity bands excised from the gels were used to identify a suitable metal in the separated samples. FeSODs or enzymes belonging to the Fe-MnSOD superfamily were detected. The spectroscopic analyses showed that only Fe is present in the SOD activity bands. We found FeSOD in <i>Synechocystis</i> sp. PCC 6803 while two forms in <i>G. violaceus</i> and <i>Geitlerinema</i> sp. ZHR1A: FeSOD1 and FeSOD2 were present. However, no active Cu/ZnSODs were identified in <i>G. violaceus</i> and <i>Geitlerinema</i> sp. ZHR1A. We have shown that selected spectroscopic techniques can be complementary to the commonly used method of staining for SOD activity in a gel. Furthermore, the occurrence of active SODs in the cyanobacteria studied is also discussed in the context of SOD evolution in oxyphotrophs. |
|---|---|
| ISSN: | 1661-6596 1422-0067 |