Isolation, Purification and Structural Identification of Red Pigment from Nuodeng Ham
In order to explore the chemical nature of red pigment in Nuodeng ham, the red pigment was extracted with 75% acetone aqueous solution and purified using a C18 solid phase extraction column, and its chemical structure was characterized by using ultraviolet-visible (UV-Vis) spectroscopy, fluorescence...
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| Format: | Article |
| Language: | English |
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China Food Publishing Company
2023-01-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-2-039.pdf |
| _version_ | 1811159477869608960 |
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| author | YANG Zijiang, ZHANG Lihong, LIAO Guozhou, TIAN Mei, LÜ Donglin, HE Ying, GE Changrong, WANG Guiying |
| author_facet | YANG Zijiang, ZHANG Lihong, LIAO Guozhou, TIAN Mei, LÜ Donglin, HE Ying, GE Changrong, WANG Guiying |
| author_sort | YANG Zijiang, ZHANG Lihong, LIAO Guozhou, TIAN Mei, LÜ Donglin, HE Ying, GE Changrong, WANG Guiying |
| collection | DOAJ |
| description | In order to explore the chemical nature of red pigment in Nuodeng ham, the red pigment was extracted with 75% acetone aqueous solution and purified using a C18 solid phase extraction column, and its chemical structure was characterized by using ultraviolet-visible (UV-Vis) spectroscopy, fluorescence spectroscopy and ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), Fourier transform infrared (FTIR) spectroscopy and nuclear magnetic resonance (NMR) spectroscopy. The UV-Vis spectrum showed a strong absorption peak at 416 nm and two weakly symmetrical absorption peaks at 546 and 584 nm, which are characteristic of metalloporphyrins. When it was excited at 420 nm, the red pigment exhibited a strong fluorescence emission peak at 590 nm and a weak fluorescence emission peak at 644 nm, which highly coincided with those of Zn protoporphyrin IX (ZnPP) standard, indicating that the metal ions in the porphyrin ring were zinc ions. ZnPP was detected by UPLC-MS/MS in both positive (m/z 625.177 9 [M + H]+) and negative ion (m/z 623.161 4 [M-H]–) modes, and the stretching vibrations of characteristic functional groups on the porphyrin ring such as –CH3, –CH2–, C=O, –C–OH in carboxyl groups, C–H on olefin and porphyrin ring C=N were observed in the FTIR spectrum. In addition, the 1H-NMR results of the red pigment were consistent with the structure of the target compound ZnPP. Therefore, the red pigment in Nuodeng ham was identified as ZnPP. The results of this study provide a theoretical basis for the color regulation of Nuodeng ham. |
| first_indexed | 2024-04-10T05:42:55Z |
| format | Article |
| id | doaj.art-21e97d3701624e4883d6f7592fbdb16a |
| institution | Directory Open Access Journal |
| issn | 1002-6630 |
| language | English |
| last_indexed | 2024-04-10T05:42:55Z |
| publishDate | 2023-01-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj.art-21e97d3701624e4883d6f7592fbdb16a2023-03-06T07:13:03ZengChina Food Publishing CompanyShipin Kexue1002-66302023-01-0144231231810.7506/spkx1002-6630-20211203-039Isolation, Purification and Structural Identification of Red Pigment from Nuodeng HamYANG Zijiang, ZHANG Lihong, LIAO Guozhou, TIAN Mei, LÜ Donglin, HE Ying, GE Changrong, WANG Guiying0(1. College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China; 2. Livestock Product Processing and Engineering Technology Research Center of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China)In order to explore the chemical nature of red pigment in Nuodeng ham, the red pigment was extracted with 75% acetone aqueous solution and purified using a C18 solid phase extraction column, and its chemical structure was characterized by using ultraviolet-visible (UV-Vis) spectroscopy, fluorescence spectroscopy and ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), Fourier transform infrared (FTIR) spectroscopy and nuclear magnetic resonance (NMR) spectroscopy. The UV-Vis spectrum showed a strong absorption peak at 416 nm and two weakly symmetrical absorption peaks at 546 and 584 nm, which are characteristic of metalloporphyrins. When it was excited at 420 nm, the red pigment exhibited a strong fluorescence emission peak at 590 nm and a weak fluorescence emission peak at 644 nm, which highly coincided with those of Zn protoporphyrin IX (ZnPP) standard, indicating that the metal ions in the porphyrin ring were zinc ions. ZnPP was detected by UPLC-MS/MS in both positive (m/z 625.177 9 [M + H]+) and negative ion (m/z 623.161 4 [M-H]–) modes, and the stretching vibrations of characteristic functional groups on the porphyrin ring such as –CH3, –CH2–, C=O, –C–OH in carboxyl groups, C–H on olefin and porphyrin ring C=N were observed in the FTIR spectrum. In addition, the 1H-NMR results of the red pigment were consistent with the structure of the target compound ZnPP. Therefore, the red pigment in Nuodeng ham was identified as ZnPP. The results of this study provide a theoretical basis for the color regulation of Nuodeng ham.https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-2-039.pdfnuodeng ham; red pigment; separation and purification; zn protoporphyrin ix; structural characterization |
| spellingShingle | YANG Zijiang, ZHANG Lihong, LIAO Guozhou, TIAN Mei, LÜ Donglin, HE Ying, GE Changrong, WANG Guiying Isolation, Purification and Structural Identification of Red Pigment from Nuodeng Ham Shipin Kexue nuodeng ham; red pigment; separation and purification; zn protoporphyrin ix; structural characterization |
| title | Isolation, Purification and Structural Identification of Red Pigment from Nuodeng Ham |
| title_full | Isolation, Purification and Structural Identification of Red Pigment from Nuodeng Ham |
| title_fullStr | Isolation, Purification and Structural Identification of Red Pigment from Nuodeng Ham |
| title_full_unstemmed | Isolation, Purification and Structural Identification of Red Pigment from Nuodeng Ham |
| title_short | Isolation, Purification and Structural Identification of Red Pigment from Nuodeng Ham |
| title_sort | isolation purification and structural identification of red pigment from nuodeng ham |
| topic | nuodeng ham; red pigment; separation and purification; zn protoporphyrin ix; structural characterization |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-2-039.pdf |
| work_keys_str_mv | AT yangzijiangzhanglihongliaoguozhoutianmeiludonglinheyinggechangrongwangguiying isolationpurificationandstructuralidentificationofredpigmentfromnuodengham |