Structuring and De-Structuring of Nanovectors from Algal Lipids: Simulated Digestion, Preliminary Antioxidant Capacity and In Vitro Tests
Biocompatible nanocarriers can be obtained by lipid extraction from natural sources such as algal biomasses, which accumulate different lipid classes depending on the employed culture media. Lipid aggregates can be distinguished according to supramolecular architecture into lamellar and nonlamellar...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/9/1847 |
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| author | Ilaria Clemente Stefania Lamponi Gabriella Tamasi Liliana Rodolfi Claudio Rossi Sandra Ristori |
| author_facet | Ilaria Clemente Stefania Lamponi Gabriella Tamasi Liliana Rodolfi Claudio Rossi Sandra Ristori |
| author_sort | Ilaria Clemente |
| collection | DOAJ |
| description | Biocompatible nanocarriers can be obtained by lipid extraction from natural sources such as algal biomasses, which accumulate different lipid classes depending on the employed culture media. Lipid aggregates can be distinguished according to supramolecular architecture into lamellar and nonlamellar structures. This distinction is mainly influenced by the lipid class and molecular packing parameter, which determine the possible values of interfacial curvature and thus the supramolecular symmetries that can be obtained. The nanosystems prepared from bio-sources are able to self-assemble into different compartmentalized structures due to their complex composition. They also present the advantage of increased carrier-target biocompatibility and are suitable to encapsulate and vehiculate poorly water-soluble compounds, e.g., natural antioxidants. Their functional properties stem from the interplay of several parameters. Following previous work, here the functionality of two series of structurally distinct lipid nanocarriers, namely liposomes and cubosomes deriving from algal biomasses with different lipid composition, is characterized. In the view of their possible use as pharmaceutical or nutraceutical formulations, both types of nanovectors were loaded with three well-known antioxidants, i.e., curcumin, α-tocopherol and piperine, and their carrier efficacy was compared considering their different structures. Firstly, carrier stability in biorelevant conditions was assessed by simulating a gastrointestinal tract model. Then, by using an integrated chemical and pharmacological approach, the functionality in terms of encapsulation efficiency, cargo bioaccessibility and kinetics of antioxidant capacity by UV-Visible spectroscopy was evaluated. Subsequently, in vitro cytotoxicity and viability tests after administration to model cell lines were performed. As a consequence of this investigation, it is possible to conclude that nanovectors from algal lipids, i.e., cubosomes and liposomes, can be efficient delivery agents for lipophilic antioxidants, being able to preserve and enhance their activity toward different targets while promoting sustained release. |
| first_indexed | 2024-03-09T22:50:03Z |
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| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T22:50:03Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-21ea100cfac54cdda2fbdf8446c95a612023-11-23T18:21:39ZengMDPI AGPharmaceutics1999-49232022-09-01149184710.3390/pharmaceutics14091847Structuring and De-Structuring of Nanovectors from Algal Lipids: Simulated Digestion, Preliminary Antioxidant Capacity and In Vitro TestsIlaria Clemente0Stefania Lamponi1Gabriella Tamasi2Liliana Rodolfi3Claudio Rossi4Sandra Ristori5Department of Biotechnology, Chemistry and Pharmacy & Center for Colloids and Surface Science (CSGI), University of Siena, Via Aldo Moro 2, 53100 Siena, ItalyDepartment of Biotechnology, Chemistry and Pharmacy & Center for Colloids and Surface Science (CSGI), University of Siena, Via Aldo Moro 2, 53100 Siena, ItalyDepartment of Biotechnology, Chemistry and Pharmacy & Center for Colloids and Surface Science (CSGI), University of Siena, Via Aldo Moro 2, 53100 Siena, ItalyDepartment of Agriculture, Food, Environment and Forestry (DAGRI), University of Florence, Piazzale delle Cascine 18, 50144 Florence, ItalyDepartment of Biotechnology, Chemistry and Pharmacy & Center for Colloids and Surface Science (CSGI), University of Siena, Via Aldo Moro 2, 53100 Siena, ItalyDepartment of Chemistry “Ugo Schiff” & Center for Colloids and Surface Science (CSGI), University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, ItalyBiocompatible nanocarriers can be obtained by lipid extraction from natural sources such as algal biomasses, which accumulate different lipid classes depending on the employed culture media. Lipid aggregates can be distinguished according to supramolecular architecture into lamellar and nonlamellar structures. This distinction is mainly influenced by the lipid class and molecular packing parameter, which determine the possible values of interfacial curvature and thus the supramolecular symmetries that can be obtained. The nanosystems prepared from bio-sources are able to self-assemble into different compartmentalized structures due to their complex composition. They also present the advantage of increased carrier-target biocompatibility and are suitable to encapsulate and vehiculate poorly water-soluble compounds, e.g., natural antioxidants. Their functional properties stem from the interplay of several parameters. Following previous work, here the functionality of two series of structurally distinct lipid nanocarriers, namely liposomes and cubosomes deriving from algal biomasses with different lipid composition, is characterized. In the view of their possible use as pharmaceutical or nutraceutical formulations, both types of nanovectors were loaded with three well-known antioxidants, i.e., curcumin, α-tocopherol and piperine, and their carrier efficacy was compared considering their different structures. Firstly, carrier stability in biorelevant conditions was assessed by simulating a gastrointestinal tract model. Then, by using an integrated chemical and pharmacological approach, the functionality in terms of encapsulation efficiency, cargo bioaccessibility and kinetics of antioxidant capacity by UV-Visible spectroscopy was evaluated. Subsequently, in vitro cytotoxicity and viability tests after administration to model cell lines were performed. As a consequence of this investigation, it is possible to conclude that nanovectors from algal lipids, i.e., cubosomes and liposomes, can be efficient delivery agents for lipophilic antioxidants, being able to preserve and enhance their activity toward different targets while promoting sustained release.https://www.mdpi.com/1999-4923/14/9/1847lipid nanocarriersalgal biomasscurcuminsimulated digestionantioxidant capacityin vitro tests |
| spellingShingle | Ilaria Clemente Stefania Lamponi Gabriella Tamasi Liliana Rodolfi Claudio Rossi Sandra Ristori Structuring and De-Structuring of Nanovectors from Algal Lipids: Simulated Digestion, Preliminary Antioxidant Capacity and In Vitro Tests Pharmaceutics lipid nanocarriers algal biomass curcumin simulated digestion antioxidant capacity in vitro tests |
| title | Structuring and De-Structuring of Nanovectors from Algal Lipids: Simulated Digestion, Preliminary Antioxidant Capacity and In Vitro Tests |
| title_full | Structuring and De-Structuring of Nanovectors from Algal Lipids: Simulated Digestion, Preliminary Antioxidant Capacity and In Vitro Tests |
| title_fullStr | Structuring and De-Structuring of Nanovectors from Algal Lipids: Simulated Digestion, Preliminary Antioxidant Capacity and In Vitro Tests |
| title_full_unstemmed | Structuring and De-Structuring of Nanovectors from Algal Lipids: Simulated Digestion, Preliminary Antioxidant Capacity and In Vitro Tests |
| title_short | Structuring and De-Structuring of Nanovectors from Algal Lipids: Simulated Digestion, Preliminary Antioxidant Capacity and In Vitro Tests |
| title_sort | structuring and de structuring of nanovectors from algal lipids simulated digestion preliminary antioxidant capacity and in vitro tests |
| topic | lipid nanocarriers algal biomass curcumin simulated digestion antioxidant capacity in vitro tests |
| url | https://www.mdpi.com/1999-4923/14/9/1847 |
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