Kielin/chordin‐like protein enhances induction of osteoblast differentiation by Bone Morphogenetic Protein‐2
Bone morphogenetic proteins (BMPs) play a key role in embryonic differentiation for osteoblast and bone formation. Kielin/chordin‐like protein (Kcp) is known to enhance the effects of BMP signaling. Here, we present ALP activity, gene expression, and calcification data demonstrating that Kcp affects...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-07-01
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| Series: | FEBS Open Bio |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/2211-5463.13652 |
| _version_ | 1797789127943389184 |
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| author | Kei Nagasaki Atsushi Yamada Kiyohito Sasa Ryutaro Kamijo |
| author_facet | Kei Nagasaki Atsushi Yamada Kiyohito Sasa Ryutaro Kamijo |
| author_sort | Kei Nagasaki |
| collection | DOAJ |
| description | Bone morphogenetic proteins (BMPs) play a key role in embryonic differentiation for osteoblast and bone formation. Kielin/chordin‐like protein (Kcp) is known to enhance the effects of BMP signaling. Here, we present ALP activity, gene expression, and calcification data demonstrating that Kcp affects the differentiation of C2C12 myoblasts into osteoblasts. We report that the presence of Kcp enhances the ability of BMP‐2 to induce the differentiation of C2C12 myoblasts into osteoblasts. Additionally, BMP‐2‐mediated stimulation of phosphorylated Smad1/5 was apparently enhanced in the presence of Kcp. The present findings may contribute to progression toward the clinical use of BMPs for treatment of bone fracture, osteoarthritis, and other similar conditions. |
| first_indexed | 2024-03-13T01:46:20Z |
| format | Article |
| id | doaj.art-21ec2d6172524eb9a8071cd063bf98a7 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-03-13T01:46:20Z |
| publishDate | 2023-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-21ec2d6172524eb9a8071cd063bf98a72023-07-03T07:24:35ZengWileyFEBS Open Bio2211-54632023-07-011371357136410.1002/2211-5463.13652Kielin/chordin‐like protein enhances induction of osteoblast differentiation by Bone Morphogenetic Protein‐2Kei Nagasaki0Atsushi Yamada1Kiyohito Sasa2Ryutaro Kamijo3Department of Biochemistry, School of Dentistry Showa University Shinagawa‐ku JapanDepartment of Biochemistry, School of Dentistry Showa University Shinagawa‐ku JapanDepartment of Biochemistry, School of Dentistry Showa University Shinagawa‐ku JapanDepartment of Biochemistry, School of Dentistry Showa University Shinagawa‐ku JapanBone morphogenetic proteins (BMPs) play a key role in embryonic differentiation for osteoblast and bone formation. Kielin/chordin‐like protein (Kcp) is known to enhance the effects of BMP signaling. Here, we present ALP activity, gene expression, and calcification data demonstrating that Kcp affects the differentiation of C2C12 myoblasts into osteoblasts. We report that the presence of Kcp enhances the ability of BMP‐2 to induce the differentiation of C2C12 myoblasts into osteoblasts. Additionally, BMP‐2‐mediated stimulation of phosphorylated Smad1/5 was apparently enhanced in the presence of Kcp. The present findings may contribute to progression toward the clinical use of BMPs for treatment of bone fracture, osteoarthritis, and other similar conditions.https://doi.org/10.1002/2211-5463.13652BMPC2C12 myoblastsKCP (kielim/chordin‐like protein)osteoarthritisosteoblasts |
| spellingShingle | Kei Nagasaki Atsushi Yamada Kiyohito Sasa Ryutaro Kamijo Kielin/chordin‐like protein enhances induction of osteoblast differentiation by Bone Morphogenetic Protein‐2 FEBS Open Bio BMP C2C12 myoblasts KCP (kielim/chordin‐like protein) osteoarthritis osteoblasts |
| title | Kielin/chordin‐like protein enhances induction of osteoblast differentiation by Bone Morphogenetic Protein‐2 |
| title_full | Kielin/chordin‐like protein enhances induction of osteoblast differentiation by Bone Morphogenetic Protein‐2 |
| title_fullStr | Kielin/chordin‐like protein enhances induction of osteoblast differentiation by Bone Morphogenetic Protein‐2 |
| title_full_unstemmed | Kielin/chordin‐like protein enhances induction of osteoblast differentiation by Bone Morphogenetic Protein‐2 |
| title_short | Kielin/chordin‐like protein enhances induction of osteoblast differentiation by Bone Morphogenetic Protein‐2 |
| title_sort | kielin chordin like protein enhances induction of osteoblast differentiation by bone morphogenetic protein 2 |
| topic | BMP C2C12 myoblasts KCP (kielim/chordin‐like protein) osteoarthritis osteoblasts |
| url | https://doi.org/10.1002/2211-5463.13652 |
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