Oral Prevalence of <i>Akkermansia muciniphila</i> Differs among Pediatric and Adult Orthodontic and Non-Orthodontic Patients
<i>Akkermansia muciniphila</i> (AM) is one of many highly abundant intestinal microbes that influences homeostasis and metabolic disorders and may also play a role in oral disorders. However, there is little evidence regarding the oral prevalence of this organism. Based upon this lack of...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/11/1/112 |
| _version_ | 1827623119320252416 |
|---|---|
| author | Ching Shen James Brigham Clawson Justin Simpson Karl Kingsley |
| author_facet | Ching Shen James Brigham Clawson Justin Simpson Karl Kingsley |
| author_sort | Ching Shen |
| collection | DOAJ |
| description | <i>Akkermansia muciniphila</i> (AM) is one of many highly abundant intestinal microbes that influences homeostasis and metabolic disorders and may also play a role in oral disorders. However, there is little evidence regarding the oral prevalence of this organism. Based upon this lack of evidence, the primary goal of this project is to survey an existing saliva repository to determine the overall prevalence of this organism and any associations with demographic or patient characteristics (age, sex, body mass index, race/ethnicity, orthodontic therapy). Using an approved protocol,, a total <i>n</i> = 141 pediatric samples from an existing saliva repository were screened using qPCR revealing 29.8% harbored AM with nearly equal distribution among males and females, <i>p</i> = 0.8347. Significantly higher percentages of pediatric, non-orthodontic patients were positive for AM (42.3%) compared with age-matched orthodontic patients (14.3%)—which were equally distributed among non-orthodontic males (42.1%) and non-orthodontic females (42.5%). In addition, analysis of the adult samples revealed that nearly equal percentages of males (18.2%) and females (16.7%) harbored detectable levels of salivary AM, <i>p</i> = 0.2035. However, a higher proportion of non-orthodontic adult samples harbored AM (21.3%) compared to orthodontic samples (12.8%, <i>p</i> = 0.0001), which was equally distributed among males and females. These results suggest that both age and the presence of orthodontic brackets may influence microbial composition and, more specifically, are associated with reduction in AM among both pediatric and adult populations from their baseline levels. |
| first_indexed | 2024-03-09T11:38:54Z |
| format | Article |
| id | doaj.art-21f4d726ba3940868dc0ccdaf3f224fa |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T11:38:54Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-21f4d726ba3940868dc0ccdaf3f224fa2023-11-30T23:36:34ZengMDPI AGMicroorganisms2076-26072023-01-0111111210.3390/microorganisms11010112Oral Prevalence of <i>Akkermansia muciniphila</i> Differs among Pediatric and Adult Orthodontic and Non-Orthodontic PatientsChing Shen0James Brigham Clawson1Justin Simpson2Karl Kingsley3Department of Advanced Education in Orthodontic Dentistry, School of Dental Medicine, University of Nevada—Las Vegas, 1700 W. Charleston Boulevard, Las Vegas, NV 89106, USADepartment of Clinical Sciences, School of Dental Medicine, University of Nevada—Las Vegas, 1700 W. Charleston Boulevard, Las Vegas, NV 89106, USADepartment of Clinical Sciences, School of Dental Medicine, University of Nevada—Las Vegas, 1700 W. Charleston Boulevard, Las Vegas, NV 89106, USADepartment of Biomedical Sciences, School of Dental Medicine, University of Nevada—Las Vegas, 1001 Shadow Lane, Las Vegas, NV 89106, USA<i>Akkermansia muciniphila</i> (AM) is one of many highly abundant intestinal microbes that influences homeostasis and metabolic disorders and may also play a role in oral disorders. However, there is little evidence regarding the oral prevalence of this organism. Based upon this lack of evidence, the primary goal of this project is to survey an existing saliva repository to determine the overall prevalence of this organism and any associations with demographic or patient characteristics (age, sex, body mass index, race/ethnicity, orthodontic therapy). Using an approved protocol,, a total <i>n</i> = 141 pediatric samples from an existing saliva repository were screened using qPCR revealing 29.8% harbored AM with nearly equal distribution among males and females, <i>p</i> = 0.8347. Significantly higher percentages of pediatric, non-orthodontic patients were positive for AM (42.3%) compared with age-matched orthodontic patients (14.3%)—which were equally distributed among non-orthodontic males (42.1%) and non-orthodontic females (42.5%). In addition, analysis of the adult samples revealed that nearly equal percentages of males (18.2%) and females (16.7%) harbored detectable levels of salivary AM, <i>p</i> = 0.2035. However, a higher proportion of non-orthodontic adult samples harbored AM (21.3%) compared to orthodontic samples (12.8%, <i>p</i> = 0.0001), which was equally distributed among males and females. These results suggest that both age and the presence of orthodontic brackets may influence microbial composition and, more specifically, are associated with reduction in AM among both pediatric and adult populations from their baseline levels.https://www.mdpi.com/2076-2607/11/1/112<i>Akkermansia muciniphila</i>saliva screeningorthodonticoral prevalence |
| spellingShingle | Ching Shen James Brigham Clawson Justin Simpson Karl Kingsley Oral Prevalence of <i>Akkermansia muciniphila</i> Differs among Pediatric and Adult Orthodontic and Non-Orthodontic Patients Microorganisms <i>Akkermansia muciniphila</i> saliva screening orthodontic oral prevalence |
| title | Oral Prevalence of <i>Akkermansia muciniphila</i> Differs among Pediatric and Adult Orthodontic and Non-Orthodontic Patients |
| title_full | Oral Prevalence of <i>Akkermansia muciniphila</i> Differs among Pediatric and Adult Orthodontic and Non-Orthodontic Patients |
| title_fullStr | Oral Prevalence of <i>Akkermansia muciniphila</i> Differs among Pediatric and Adult Orthodontic and Non-Orthodontic Patients |
| title_full_unstemmed | Oral Prevalence of <i>Akkermansia muciniphila</i> Differs among Pediatric and Adult Orthodontic and Non-Orthodontic Patients |
| title_short | Oral Prevalence of <i>Akkermansia muciniphila</i> Differs among Pediatric and Adult Orthodontic and Non-Orthodontic Patients |
| title_sort | oral prevalence of i akkermansia muciniphila i differs among pediatric and adult orthodontic and non orthodontic patients |
| topic | <i>Akkermansia muciniphila</i> saliva screening orthodontic oral prevalence |
| url | https://www.mdpi.com/2076-2607/11/1/112 |
| work_keys_str_mv | AT chingshen oralprevalenceofiakkermansiamuciniphilaidiffersamongpediatricandadultorthodonticandnonorthodonticpatients AT jamesbrighamclawson oralprevalenceofiakkermansiamuciniphilaidiffersamongpediatricandadultorthodonticandnonorthodonticpatients AT justinsimpson oralprevalenceofiakkermansiamuciniphilaidiffersamongpediatricandadultorthodonticandnonorthodonticpatients AT karlkingsley oralprevalenceofiakkermansiamuciniphilaidiffersamongpediatricandadultorthodonticandnonorthodonticpatients |