Sex-Dependent Gene Expression in Human Pluripotent Stem Cells
Males and females have a variety of sexually dimorphic traits, most of which result from hormonal differences. However, differences between male and female embryos initiate very early in development, before hormonal influence begins, suggesting the presence of genetically driven sexual dimorphisms....
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014-08-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124714005804 |
| _version_ | 1811306112113180672 |
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| author | Daniel Ronen Nissim Benvenisty |
| author_facet | Daniel Ronen Nissim Benvenisty |
| author_sort | Daniel Ronen |
| collection | DOAJ |
| description | Males and females have a variety of sexually dimorphic traits, most of which result from hormonal differences. However, differences between male and female embryos initiate very early in development, before hormonal influence begins, suggesting the presence of genetically driven sexual dimorphisms. By comparing the gene expression profiles of male and X-inactivated female human pluripotent stem cells, we detected Y-chromosome-driven effects. We discovered that the sex-determining gene SRY is expressed in human male pluripotent stem cells and is induced by reprogramming. In addition, we detected more than 200 differentially expressed autosomal genes in male and female embryonic stem cells. Some of these genes are involved in steroid metabolism pathways and lead to sex-dependent differentiation in response to the estrogen precursor estrone. Thus, we propose that the presence of the Y chromosome and specifically SRY may drive sex-specific differences in the growth and differentiation of pluripotent stem cells. |
| first_indexed | 2024-04-13T08:38:36Z |
| format | Article |
| id | doaj.art-21f54b511b064e02a68ad4b8e6538d18 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-04-13T08:38:36Z |
| publishDate | 2014-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-21f54b511b064e02a68ad4b8e6538d182022-12-22T02:53:59ZengElsevierCell Reports2211-12472014-08-018492393210.1016/j.celrep.2014.07.013Sex-Dependent Gene Expression in Human Pluripotent Stem CellsDaniel Ronen0Nissim Benvenisty1Stem Cell Unit, Department of Genetics, Institute of Life Sciences, The Hebrew University, Jerusalem 91904, IsraelStem Cell Unit, Department of Genetics, Institute of Life Sciences, The Hebrew University, Jerusalem 91904, IsraelMales and females have a variety of sexually dimorphic traits, most of which result from hormonal differences. However, differences between male and female embryos initiate very early in development, before hormonal influence begins, suggesting the presence of genetically driven sexual dimorphisms. By comparing the gene expression profiles of male and X-inactivated female human pluripotent stem cells, we detected Y-chromosome-driven effects. We discovered that the sex-determining gene SRY is expressed in human male pluripotent stem cells and is induced by reprogramming. In addition, we detected more than 200 differentially expressed autosomal genes in male and female embryonic stem cells. Some of these genes are involved in steroid metabolism pathways and lead to sex-dependent differentiation in response to the estrogen precursor estrone. Thus, we propose that the presence of the Y chromosome and specifically SRY may drive sex-specific differences in the growth and differentiation of pluripotent stem cells.http://www.sciencedirect.com/science/article/pii/S2211124714005804 |
| spellingShingle | Daniel Ronen Nissim Benvenisty Sex-Dependent Gene Expression in Human Pluripotent Stem Cells Cell Reports |
| title | Sex-Dependent Gene Expression in Human Pluripotent Stem Cells |
| title_full | Sex-Dependent Gene Expression in Human Pluripotent Stem Cells |
| title_fullStr | Sex-Dependent Gene Expression in Human Pluripotent Stem Cells |
| title_full_unstemmed | Sex-Dependent Gene Expression in Human Pluripotent Stem Cells |
| title_short | Sex-Dependent Gene Expression in Human Pluripotent Stem Cells |
| title_sort | sex dependent gene expression in human pluripotent stem cells |
| url | http://www.sciencedirect.com/science/article/pii/S2211124714005804 |
| work_keys_str_mv | AT danielronen sexdependentgeneexpressioninhumanpluripotentstemcells AT nissimbenvenisty sexdependentgeneexpressioninhumanpluripotentstemcells |