Drug Induced Sialorrhea and Microfluidic-Chip-Electrophoretic Analysis of Engorged Adult Female Tick Saliva of Haemaphysalis longicornis (Acari: Ixodidae)
Background: The aim of the present study was to induce salivation in Haemaphysalis longicornis to increase saliva production and to characterize the collection of proteins present in the collected saliva using on-chip-electrophoresis. Methods: Saliva of adult female engorged H. longicornis was colle...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2017-04-01
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| Series: | Journal of Arthropod-Borne Diseases |
| Subjects: | |
| Online Access: | https://jad.tums.ac.ir/index.php/jad/article/view/384 |
| _version_ | 1828741600498417664 |
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| author | Mohammad Saiful Islam Myung Jo You |
| author_facet | Mohammad Saiful Islam Myung Jo You |
| author_sort | Mohammad Saiful Islam |
| collection | DOAJ |
| description | Background: The aim of the present study was to induce salivation in Haemaphysalis longicornis to increase saliva production and to characterize the collection of proteins present in the collected saliva using on-chip-electrophoresis.
Methods: Saliva of adult female engorged H. longicornis was collected by treatment with 0.2% dopamine hydrochloride. All protein samples were characterized by SDS-PAGE electrophoresis using a microfluidic High Sensitivity Protein Assay 250 kit by 2100 Bioanalyzer (Agilent Technologies, USA) under non-reducing conditions.
Results: The average salivary protein concentration was 0.169 µg/µl/tick and saliva secretion decreased with increased time of tick detachment from the host. Saliva secretion volume increased to 3.56 µl in the group of ticks with a body weight between 301–350 mg as compared to higher and lower body weight groups. On-chip-electrophoresis results show 13 distinct bands ranging from 9.9 to 294 kDa.
Conclusion: Based on molecular weight, the putative salivary proteins are comprised of proline-rich proteins, triabin, apyrase members of the 12-kDa protein family, platelet inhibitors and anti-inflammatory proteins as tick saliva contains anti-inflammatory components. |
| first_indexed | 2024-04-13T01:02:58Z |
| format | Article |
| id | doaj.art-21f6ca0435254f30aa5fb457f9b35de5 |
| institution | Directory Open Access Journal |
| issn | 1735-7179 2322-2271 |
| language | English |
| last_indexed | 2024-04-13T01:02:58Z |
| publishDate | 2017-04-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Journal of Arthropod-Borne Diseases |
| spelling | doaj.art-21f6ca0435254f30aa5fb457f9b35de52022-12-22T03:09:25ZengTehran University of Medical SciencesJournal of Arthropod-Borne Diseases1735-71792322-22712017-04-01111306Drug Induced Sialorrhea and Microfluidic-Chip-Electrophoretic Analysis of Engorged Adult Female Tick Saliva of Haemaphysalis longicornis (Acari: Ixodidae)Mohammad Saiful Islam0Myung Jo You1Laboratory of Veterinary Parasitology, College of Veterinary Medicine and Bio-safety Research Centre, Chonbuk National University, Jeonju, Republic of KoreaDepartment of Medicine Surgery and Obstetrics, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University, Dinajpur, BangladeshBackground: The aim of the present study was to induce salivation in Haemaphysalis longicornis to increase saliva production and to characterize the collection of proteins present in the collected saliva using on-chip-electrophoresis. Methods: Saliva of adult female engorged H. longicornis was collected by treatment with 0.2% dopamine hydrochloride. All protein samples were characterized by SDS-PAGE electrophoresis using a microfluidic High Sensitivity Protein Assay 250 kit by 2100 Bioanalyzer (Agilent Technologies, USA) under non-reducing conditions. Results: The average salivary protein concentration was 0.169 µg/µl/tick and saliva secretion decreased with increased time of tick detachment from the host. Saliva secretion volume increased to 3.56 µl in the group of ticks with a body weight between 301–350 mg as compared to higher and lower body weight groups. On-chip-electrophoresis results show 13 distinct bands ranging from 9.9 to 294 kDa. Conclusion: Based on molecular weight, the putative salivary proteins are comprised of proline-rich proteins, triabin, apyrase members of the 12-kDa protein family, platelet inhibitors and anti-inflammatory proteins as tick saliva contains anti-inflammatory components.https://jad.tums.ac.ir/index.php/jad/article/view/384Haemaphysalis longicornisInductionOn-chip-electrophoresisSalivation |
| spellingShingle | Mohammad Saiful Islam Myung Jo You Drug Induced Sialorrhea and Microfluidic-Chip-Electrophoretic Analysis of Engorged Adult Female Tick Saliva of Haemaphysalis longicornis (Acari: Ixodidae) Journal of Arthropod-Borne Diseases Haemaphysalis longicornis Induction On-chip-electrophoresis Salivation |
| title | Drug Induced Sialorrhea and Microfluidic-Chip-Electrophoretic Analysis of Engorged Adult Female Tick Saliva of Haemaphysalis longicornis (Acari: Ixodidae) |
| title_full | Drug Induced Sialorrhea and Microfluidic-Chip-Electrophoretic Analysis of Engorged Adult Female Tick Saliva of Haemaphysalis longicornis (Acari: Ixodidae) |
| title_fullStr | Drug Induced Sialorrhea and Microfluidic-Chip-Electrophoretic Analysis of Engorged Adult Female Tick Saliva of Haemaphysalis longicornis (Acari: Ixodidae) |
| title_full_unstemmed | Drug Induced Sialorrhea and Microfluidic-Chip-Electrophoretic Analysis of Engorged Adult Female Tick Saliva of Haemaphysalis longicornis (Acari: Ixodidae) |
| title_short | Drug Induced Sialorrhea and Microfluidic-Chip-Electrophoretic Analysis of Engorged Adult Female Tick Saliva of Haemaphysalis longicornis (Acari: Ixodidae) |
| title_sort | drug induced sialorrhea and microfluidic chip electrophoretic analysis of engorged adult female tick saliva of haemaphysalis longicornis acari ixodidae |
| topic | Haemaphysalis longicornis Induction On-chip-electrophoresis Salivation |
| url | https://jad.tums.ac.ir/index.php/jad/article/view/384 |
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