Expression of full-length human alkylglycerol monooxygenase and fragments in Escherichia coli

Expression of full-length human alkylglycerol monooxygenase and fragments in Escherichia coli

Alkylglycerol monooxygenase (AGMO; EC 1.14.16.5) is the only enzyme known to cleave the O-alkyl ether bond of alkylglycerols in humans. It is an integral membrane protein with nine predicted transmembrane domains. We attempted to express and purify full-length and truncated forms of AGMO in Escheric...

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Bibliographic Details
Main Authors: Mayer Matthias, Keller Markus A., Watschinger Katrin, Werner-Felmayer Gabriele, Werner Ernst R., Golderer Georg
Format: Article
Language:English
Published: De Gruyter 2013-06-01
Series:Pteridines
Subjects:
alkylglycerol monooxygenase
hydrophobicity
oligomerisation
tetrahydrobiopterin
enzymes: alkylglycerol monooxygenase, gene symbol agmo, systematic name 1-alkyl-sn-glycerol, tetrahydrobiopterin:oxygen oxidoreductase (ec 1.14.16.5)
Online Access:https://doi.org/10.1515/pterid-2013-0014

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