Prussian blue nanocubes as a multimodal contrast agent for image-guided stem cell therapy of the spinal cord
Translation of stem cell therapies to treat injuries and diseases of the spinal cord is hindered by lack of real-time monitoring techniques to guide regenerative therapies intra- and postoperatively. Thus, we developed an ultrasound (US), photoacoustic (PA), and magnetic resonance (MR) imaging appro...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-06-01
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| Series: | Photoacoustics |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213597920300069 |
| _version_ | 1818961366877208576 |
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| author | Kelsey P. Kubelick Stanislav Y. Emelianov |
| author_facet | Kelsey P. Kubelick Stanislav Y. Emelianov |
| author_sort | Kelsey P. Kubelick |
| collection | DOAJ |
| description | Translation of stem cell therapies to treat injuries and diseases of the spinal cord is hindered by lack of real-time monitoring techniques to guide regenerative therapies intra- and postoperatively. Thus, we developed an ultrasound (US), photoacoustic (PA), and magnetic resonance (MR) imaging approach augmented with Prussian blue nanocubes (PBNCs) to guide stem cell injections intraoperatively and monitor stem cell therapies in the spinal cord postoperatively. Per the clinical procedure, a multi-level laminectomy was performed in rats ex vivo, and PBNC-labeled stem cells were injected directly into the spinal cord while US/PA images were acquired. US/PA/MR images were also acquired post-surgery. Several features of the imaging approach were demonstrated including detection of low stem cell concentrations, real-time needle guidance and feedback on stem cell delivery, and good agreement between US/PA/MR images. These benefits span intra- and postoperative environments to support future development of this imaging tool. |
| first_indexed | 2024-12-20T12:12:18Z |
| format | Article |
| id | doaj.art-21fa0cbc2e3e406189ccc82728f47e3e |
| institution | Directory Open Access Journal |
| issn | 2213-5979 |
| language | English |
| last_indexed | 2024-12-20T12:12:18Z |
| publishDate | 2020-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Photoacoustics |
| spelling | doaj.art-21fa0cbc2e3e406189ccc82728f47e3e2022-12-21T19:41:14ZengElsevierPhotoacoustics2213-59792020-06-0118100166Prussian blue nanocubes as a multimodal contrast agent for image-guided stem cell therapy of the spinal cordKelsey P. Kubelick0Stanislav Y. Emelianov1Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University School of Medicine, 313 Ferst Dr NW, Atlanta, GA, 30332, USA; School of Electrical and Computer Engineering, Georgia Institute of Technology, 777 Atlantic Drive, Atlanta, GA, 30332, USAWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University School of Medicine, 313 Ferst Dr NW, Atlanta, GA, 30332, USA; School of Electrical and Computer Engineering, Georgia Institute of Technology, 777 Atlantic Drive, Atlanta, GA, 30332, USA; Corresponding author at: School of Electrical and Computer Engineering, Georgia Institute of Technology, 777 Atlantic Drive, Atlanta, GA, 30332, USA.Translation of stem cell therapies to treat injuries and diseases of the spinal cord is hindered by lack of real-time monitoring techniques to guide regenerative therapies intra- and postoperatively. Thus, we developed an ultrasound (US), photoacoustic (PA), and magnetic resonance (MR) imaging approach augmented with Prussian blue nanocubes (PBNCs) to guide stem cell injections intraoperatively and monitor stem cell therapies in the spinal cord postoperatively. Per the clinical procedure, a multi-level laminectomy was performed in rats ex vivo, and PBNC-labeled stem cells were injected directly into the spinal cord while US/PA images were acquired. US/PA/MR images were also acquired post-surgery. Several features of the imaging approach were demonstrated including detection of low stem cell concentrations, real-time needle guidance and feedback on stem cell delivery, and good agreement between US/PA/MR images. These benefits span intra- and postoperative environments to support future development of this imaging tool.http://www.sciencedirect.com/science/article/pii/S2213597920300069Photoacoustic imagingUltrasoundMagnetic resonance imagingMultimodal imagingSpinal cordStem cells |
| spellingShingle | Kelsey P. Kubelick Stanislav Y. Emelianov Prussian blue nanocubes as a multimodal contrast agent for image-guided stem cell therapy of the spinal cord Photoacoustics Photoacoustic imaging Ultrasound Magnetic resonance imaging Multimodal imaging Spinal cord Stem cells |
| title | Prussian blue nanocubes as a multimodal contrast agent for image-guided stem cell therapy of the spinal cord |
| title_full | Prussian blue nanocubes as a multimodal contrast agent for image-guided stem cell therapy of the spinal cord |
| title_fullStr | Prussian blue nanocubes as a multimodal contrast agent for image-guided stem cell therapy of the spinal cord |
| title_full_unstemmed | Prussian blue nanocubes as a multimodal contrast agent for image-guided stem cell therapy of the spinal cord |
| title_short | Prussian blue nanocubes as a multimodal contrast agent for image-guided stem cell therapy of the spinal cord |
| title_sort | prussian blue nanocubes as a multimodal contrast agent for image guided stem cell therapy of the spinal cord |
| topic | Photoacoustic imaging Ultrasound Magnetic resonance imaging Multimodal imaging Spinal cord Stem cells |
| url | http://www.sciencedirect.com/science/article/pii/S2213597920300069 |
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