Biventricular differences in β-adrenergic receptor signaling following burn injury.
Burn injury detrimentally affects the myocardium, primarily due to over-activation of β-adrenergic receptors (β-AR). Autopsy reports from our institution reveal that patients often suffer from right ventricle (RV) failure. Since burn injury affects β-AR signaling in the left ventricle (LV), we propo...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5726759?pdf=render |
| _version_ | 1811261725648879616 |
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| author | Ashley N Guillory Robert P Clayton Anesh Prasai Amina El Ayadi David N Herndon Celeste C Finnerty |
| author_facet | Ashley N Guillory Robert P Clayton Anesh Prasai Amina El Ayadi David N Herndon Celeste C Finnerty |
| author_sort | Ashley N Guillory |
| collection | DOAJ |
| description | Burn injury detrimentally affects the myocardium, primarily due to over-activation of β-adrenergic receptors (β-AR). Autopsy reports from our institution reveal that patients often suffer from right ventricle (RV) failure. Since burn injury affects β-AR signaling in the left ventricle (LV), we proposed that β-AR signaling may also be altered in the RV. A rodent model with a scald burn of 60% of the total body surface area was used to test this hypothesis. Ventricles were isolated 7 days post-burn. We examined the expression of β-ARs via Western blotting and the mRNA expression of downstream signaling proteins via qRT-PCR. Cyclic adenosine monophosphate (cAMP) production and protein kinase A (PKA) activity were measured in membrane and cytosolic fractions, respectively, using enzyme immunoassay kits. β1-AR protein expression was significantly increased in the RV following burn injury compared to non-burned RV but not in the LV (p = 0.0022). In contrast, β2-AR expression was unaltered among the groups while Gαi expression was significantly higher in the LV post-burn (p = 0.023). B-arrestin-1 and G-protein coupled receptor kinase-2 mRNA expression were significantly increased in the left ventricle post-burn (p = 0.001, p<0.0001, respectively). cAMP production and PKA activity were significantly lower in the LV post-burn (p = 0.0063, 0.0042, respectively). These data indicate that burn injury affects the β-AR signaling pathway in the RV independently of the LV. Additionally, non-canonical β-AR signaling may be activated in the RV as cAMP production and PKA activity were unchanged despite changes in β1-AR protein expression. |
| first_indexed | 2024-04-12T19:10:55Z |
| format | Article |
| id | doaj.art-21fdfb3c506c49748d6c356982fbe795 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T19:10:55Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-21fdfb3c506c49748d6c356982fbe7952022-12-22T03:19:53ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-011212e018952710.1371/journal.pone.0189527Biventricular differences in β-adrenergic receptor signaling following burn injury.Ashley N GuilloryRobert P ClaytonAnesh PrasaiAmina El AyadiDavid N HerndonCeleste C FinnertyBurn injury detrimentally affects the myocardium, primarily due to over-activation of β-adrenergic receptors (β-AR). Autopsy reports from our institution reveal that patients often suffer from right ventricle (RV) failure. Since burn injury affects β-AR signaling in the left ventricle (LV), we proposed that β-AR signaling may also be altered in the RV. A rodent model with a scald burn of 60% of the total body surface area was used to test this hypothesis. Ventricles were isolated 7 days post-burn. We examined the expression of β-ARs via Western blotting and the mRNA expression of downstream signaling proteins via qRT-PCR. Cyclic adenosine monophosphate (cAMP) production and protein kinase A (PKA) activity were measured in membrane and cytosolic fractions, respectively, using enzyme immunoassay kits. β1-AR protein expression was significantly increased in the RV following burn injury compared to non-burned RV but not in the LV (p = 0.0022). In contrast, β2-AR expression was unaltered among the groups while Gαi expression was significantly higher in the LV post-burn (p = 0.023). B-arrestin-1 and G-protein coupled receptor kinase-2 mRNA expression were significantly increased in the left ventricle post-burn (p = 0.001, p<0.0001, respectively). cAMP production and PKA activity were significantly lower in the LV post-burn (p = 0.0063, 0.0042, respectively). These data indicate that burn injury affects the β-AR signaling pathway in the RV independently of the LV. Additionally, non-canonical β-AR signaling may be activated in the RV as cAMP production and PKA activity were unchanged despite changes in β1-AR protein expression.http://europepmc.org/articles/PMC5726759?pdf=render |
| spellingShingle | Ashley N Guillory Robert P Clayton Anesh Prasai Amina El Ayadi David N Herndon Celeste C Finnerty Biventricular differences in β-adrenergic receptor signaling following burn injury. PLoS ONE |
| title | Biventricular differences in β-adrenergic receptor signaling following burn injury. |
| title_full | Biventricular differences in β-adrenergic receptor signaling following burn injury. |
| title_fullStr | Biventricular differences in β-adrenergic receptor signaling following burn injury. |
| title_full_unstemmed | Biventricular differences in β-adrenergic receptor signaling following burn injury. |
| title_short | Biventricular differences in β-adrenergic receptor signaling following burn injury. |
| title_sort | biventricular differences in β adrenergic receptor signaling following burn injury |
| url | http://europepmc.org/articles/PMC5726759?pdf=render |
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