The Antimicrobial, Antioxidant, and Anticancer Activity of Greenly Synthesized Selenium and Zinc Composite Nanoparticles Using <i>Ephedra aphylla</i> Extract
The current work aimed to synthesize selenium and zinc nanoparticles using the aqueous extract of <i>Ephedra aphylla</i> as a valuable medicinal plant. The prepared nanoparticles were characterized by TEM, zeta potential, and changes in the phytochemical constituents. Hence, the phenolic...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/3/470 |
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| author | Mustafa Mohsen El-Zayat Mostafa M. Eraqi Hani Alrefai Ayman Y. El-Khateeb Marwan A. Ibrahim Hashim M. Aljohani Maher M. Aljohani Moustafa Mohammed Elshaer |
| author_facet | Mustafa Mohsen El-Zayat Mostafa M. Eraqi Hani Alrefai Ayman Y. El-Khateeb Marwan A. Ibrahim Hashim M. Aljohani Maher M. Aljohani Moustafa Mohammed Elshaer |
| author_sort | Mustafa Mohsen El-Zayat |
| collection | DOAJ |
| description | The current work aimed to synthesize selenium and zinc nanoparticles using the aqueous extract of <i>Ephedra aphylla</i> as a valuable medicinal plant. The prepared nanoparticles were characterized by TEM, zeta potential, and changes in the phytochemical constituents. Hence, the phenolic, flavonoid, and tannin contents were reduced in the case of the prepared samples of nanoparticles than the original values in the aqueous extract. The prepared extract of <i>Ephedra aphylla</i> and its selenium and zinc nanoparticles showed high potency as antioxidant agents as a result of the DPPH<sup>•</sup> assay. The samples were assessed as anticancer agents against six tumor cells and a normal lung fibroblast (WI-38) cell line. The selenium nanoparticles of <i>Ephedra aphylla</i> extract revealed very strong cytotoxicity against HePG-2 cells (inhibitory concentration (IC<sub>50</sub>) = 7.56 ± 0.6 µg/mL), HCT-116 cells (IC<sub>50</sub> = 10.02 ± 0.9 µg/mL), and HeLa cells (IC<sub>50</sub> = 9.23 ± 0.8 µg/mL). The samples were evaluated as antimicrobial agents against bacterial and fungal strains. Thus, selenium nanoparticles showed potent activities against Gram-negative strains (<i>Salmonella typhimurium</i>, <i>Pseudomonas aeruginosa</i>, <i>Klebsiella pneumoniae</i>, and <i>Escherichia coli</i>), Gram-positive strains <i>(Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus</i>, and <i>Staphylococcus epidermidis</i>), and the fungal strain <i>Candida albicans</i>. In conclusion, the preparation of nanoparticles of either selenium or zinc is crucial for improved biological characteristics. |
| first_indexed | 2024-03-10T13:00:41Z |
| format | Article |
| id | doaj.art-2200ea8b86734668a2692829c36c5827 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T13:00:41Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-2200ea8b86734668a2692829c36c58272023-11-21T11:30:15ZengMDPI AGBiomolecules2218-273X2021-03-0111347010.3390/biom11030470The Antimicrobial, Antioxidant, and Anticancer Activity of Greenly Synthesized Selenium and Zinc Composite Nanoparticles Using <i>Ephedra aphylla</i> ExtractMustafa Mohsen El-Zayat0Mostafa M. Eraqi1Hani Alrefai2Ayman Y. El-Khateeb3Marwan A. Ibrahim4Hashim M. Aljohani5Maher M. Aljohani6Moustafa Mohammed Elshaer7Unit of Genetic Engineering and Biotechnology, Faculty of Science, Mansoura University, Mansoura City 35516, EgyptNational Research Center, Department of Microbiology and Immunology, Veterinary Research Division, Dokki Giza 12622, EgyptMedical Biochemistry Department, Faculty of Medicine, Mansoura University, Mansoura City 35516, EgyptDepartment of Agricultural Chemistry, Faculty of Agriculture, Mansoura University, Mansoura City 35516, EgyptDepartment of Biology, College of Science, Majmaah University, Majmaah 11952, Saudi ArabiaDepartment of Molecular Genetics and Biochemistry, College of Medicine, University of Cincinnati, Cincinnati, OH 45221, USADepartment of Pathology, College of Medicine, Taibah University, Medina City 42353, Saudi ArabiaDepartment of Microbiology at Specialized Medical Hospital, Mansoura University, Mansoura City 35516, EgyptThe current work aimed to synthesize selenium and zinc nanoparticles using the aqueous extract of <i>Ephedra aphylla</i> as a valuable medicinal plant. The prepared nanoparticles were characterized by TEM, zeta potential, and changes in the phytochemical constituents. Hence, the phenolic, flavonoid, and tannin contents were reduced in the case of the prepared samples of nanoparticles than the original values in the aqueous extract. The prepared extract of <i>Ephedra aphylla</i> and its selenium and zinc nanoparticles showed high potency as antioxidant agents as a result of the DPPH<sup>•</sup> assay. The samples were assessed as anticancer agents against six tumor cells and a normal lung fibroblast (WI-38) cell line. The selenium nanoparticles of <i>Ephedra aphylla</i> extract revealed very strong cytotoxicity against HePG-2 cells (inhibitory concentration (IC<sub>50</sub>) = 7.56 ± 0.6 µg/mL), HCT-116 cells (IC<sub>50</sub> = 10.02 ± 0.9 µg/mL), and HeLa cells (IC<sub>50</sub> = 9.23 ± 0.8 µg/mL). The samples were evaluated as antimicrobial agents against bacterial and fungal strains. Thus, selenium nanoparticles showed potent activities against Gram-negative strains (<i>Salmonella typhimurium</i>, <i>Pseudomonas aeruginosa</i>, <i>Klebsiella pneumoniae</i>, and <i>Escherichia coli</i>), Gram-positive strains <i>(Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus</i>, and <i>Staphylococcus epidermidis</i>), and the fungal strain <i>Candida albicans</i>. In conclusion, the preparation of nanoparticles of either selenium or zinc is crucial for improved biological characteristics.https://www.mdpi.com/2218-273X/11/3/470<i>Ephedra aphylla</i>aqueous extractseleniumzincnanoparticlesantioxidant |
| spellingShingle | Mustafa Mohsen El-Zayat Mostafa M. Eraqi Hani Alrefai Ayman Y. El-Khateeb Marwan A. Ibrahim Hashim M. Aljohani Maher M. Aljohani Moustafa Mohammed Elshaer The Antimicrobial, Antioxidant, and Anticancer Activity of Greenly Synthesized Selenium and Zinc Composite Nanoparticles Using <i>Ephedra aphylla</i> Extract Biomolecules <i>Ephedra aphylla</i> aqueous extract selenium zinc nanoparticles antioxidant |
| title | The Antimicrobial, Antioxidant, and Anticancer Activity of Greenly Synthesized Selenium and Zinc Composite Nanoparticles Using <i>Ephedra aphylla</i> Extract |
| title_full | The Antimicrobial, Antioxidant, and Anticancer Activity of Greenly Synthesized Selenium and Zinc Composite Nanoparticles Using <i>Ephedra aphylla</i> Extract |
| title_fullStr | The Antimicrobial, Antioxidant, and Anticancer Activity of Greenly Synthesized Selenium and Zinc Composite Nanoparticles Using <i>Ephedra aphylla</i> Extract |
| title_full_unstemmed | The Antimicrobial, Antioxidant, and Anticancer Activity of Greenly Synthesized Selenium and Zinc Composite Nanoparticles Using <i>Ephedra aphylla</i> Extract |
| title_short | The Antimicrobial, Antioxidant, and Anticancer Activity of Greenly Synthesized Selenium and Zinc Composite Nanoparticles Using <i>Ephedra aphylla</i> Extract |
| title_sort | antimicrobial antioxidant and anticancer activity of greenly synthesized selenium and zinc composite nanoparticles using i ephedra aphylla i extract |
| topic | <i>Ephedra aphylla</i> aqueous extract selenium zinc nanoparticles antioxidant |
| url | https://www.mdpi.com/2218-273X/11/3/470 |
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