Optimizing heat inactivation for SARS-CoV-2 at 95 °C and its implications: A standardized approach
Background: Standardized and validated heat inactivation procedure for Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are not available. For heat inactivation, various protocols were reported to prepare External Quality Assessment Programme (EQAP) samples without direct comparison betw...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | Heliyon |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024044025 |
| _version_ | 1797203010724560896 |
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| author | Gannon C.K. Mak Stephen S.Y. Lau Kitty K.Y. Wong Eunice K.Y. Than Anita Y.Y. Ng Derek L.L. Hung |
| author_facet | Gannon C.K. Mak Stephen S.Y. Lau Kitty K.Y. Wong Eunice K.Y. Than Anita Y.Y. Ng Derek L.L. Hung |
| author_sort | Gannon C.K. Mak |
| collection | DOAJ |
| description | Background: Standardized and validated heat inactivation procedure for Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are not available. For heat inactivation, various protocols were reported to prepare External Quality Assessment Programme (EQAP) samples without direct comparison between different durations. Objective: To assess the heat inactivation procedures against SARS-CoV-2. The efficacy of the optimized condition was reflected by the results from laboratories testing the EQAP samples. Study design: The SARS-CoV-2 strain was exposed to 95 °C in a water bath for three different time intervals, 5 min, 10 min and 15 min, respectively. The efficacy of inactivation was confirmed by the absence of cytopathic effects and decreasing viral load in 3 successive cell line passages. The viral stock inactivated by the optimal time interval was dispatched to EQAP participants and the result returned were analyzed. Results: All of the three conditions were capable of inactivating the SARS-CoV-2 of viral load at around cycle threshold value of 10. When the 95 °C 10 min condition was chosen to prepare SARS-CoV-2 EQAP samples, they showed sufficient homogeneity and stability. High degree of consensus was observed among EQAP participants in all samples dispatched. Conclusions: The conditions evaluated in the present study could be helpful for laboratories in preparing SARS-CoV-2 EQAP samples. |
| first_indexed | 2024-04-24T08:12:32Z |
| format | Article |
| id | doaj.art-2201e2b2a6054bdaa79794cae0eaae97 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T08:12:32Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-2201e2b2a6054bdaa79794cae0eaae972024-04-17T04:49:25ZengElsevierHeliyon2405-84402024-04-01108e28371Optimizing heat inactivation for SARS-CoV-2 at 95 °C and its implications: A standardized approachGannon C.K. Mak0Stephen S.Y. Lau1Kitty K.Y. Wong2Eunice K.Y. Than3Anita Y.Y. Ng4Derek L.L. Hung5Corresponding author. 9/F, Public Health Laboratory Centre 382 Nam Cheong Street Shek Kip Mei Kowloon, Hong Kong Special Administrative Region .; All from Microbiology Division, Public Health Laboratory Services Branch, Centre for Health Protection, Department of Health, Hong Kong Special Administrative RegionAll from Microbiology Division, Public Health Laboratory Services Branch, Centre for Health Protection, Department of Health, Hong Kong Special Administrative RegionAll from Microbiology Division, Public Health Laboratory Services Branch, Centre for Health Protection, Department of Health, Hong Kong Special Administrative RegionAll from Microbiology Division, Public Health Laboratory Services Branch, Centre for Health Protection, Department of Health, Hong Kong Special Administrative RegionAll from Microbiology Division, Public Health Laboratory Services Branch, Centre for Health Protection, Department of Health, Hong Kong Special Administrative RegionAll from Microbiology Division, Public Health Laboratory Services Branch, Centre for Health Protection, Department of Health, Hong Kong Special Administrative RegionBackground: Standardized and validated heat inactivation procedure for Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are not available. For heat inactivation, various protocols were reported to prepare External Quality Assessment Programme (EQAP) samples without direct comparison between different durations. Objective: To assess the heat inactivation procedures against SARS-CoV-2. The efficacy of the optimized condition was reflected by the results from laboratories testing the EQAP samples. Study design: The SARS-CoV-2 strain was exposed to 95 °C in a water bath for three different time intervals, 5 min, 10 min and 15 min, respectively. The efficacy of inactivation was confirmed by the absence of cytopathic effects and decreasing viral load in 3 successive cell line passages. The viral stock inactivated by the optimal time interval was dispatched to EQAP participants and the result returned were analyzed. Results: All of the three conditions were capable of inactivating the SARS-CoV-2 of viral load at around cycle threshold value of 10. When the 95 °C 10 min condition was chosen to prepare SARS-CoV-2 EQAP samples, they showed sufficient homogeneity and stability. High degree of consensus was observed among EQAP participants in all samples dispatched. Conclusions: The conditions evaluated in the present study could be helpful for laboratories in preparing SARS-CoV-2 EQAP samples.http://www.sciencedirect.com/science/article/pii/S2405844024044025 |
| spellingShingle | Gannon C.K. Mak Stephen S.Y. Lau Kitty K.Y. Wong Eunice K.Y. Than Anita Y.Y. Ng Derek L.L. Hung Optimizing heat inactivation for SARS-CoV-2 at 95 °C and its implications: A standardized approach Heliyon |
| title | Optimizing heat inactivation for SARS-CoV-2 at 95 °C and its implications: A standardized approach |
| title_full | Optimizing heat inactivation for SARS-CoV-2 at 95 °C and its implications: A standardized approach |
| title_fullStr | Optimizing heat inactivation for SARS-CoV-2 at 95 °C and its implications: A standardized approach |
| title_full_unstemmed | Optimizing heat inactivation for SARS-CoV-2 at 95 °C and its implications: A standardized approach |
| title_short | Optimizing heat inactivation for SARS-CoV-2 at 95 °C and its implications: A standardized approach |
| title_sort | optimizing heat inactivation for sars cov 2 at 95 °c and its implications a standardized approach |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024044025 |
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