Plant-Specific Insert (PSI)-Mediated Vacuolar Sorting Is Not Disrupted in Arabidopsis Mutant with Abnormal ER Morphology
The endomembrane system in plant cells enables the cell to manage and coordinate a variety of membranous compartments so that they and their contents arrive at the right location. The secretory pathway is an essential part of this complex network and has its gateway at the Endoplasmic Reticulum. The...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | International Journal of Plant Biology |
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| Online Access: | https://www.mdpi.com/2037-0164/14/4/75 |
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| author | Tatiana Cardoso Miguel Sampaio João Neves Sofia Oliveira Inês Moura Ana Séneca José Pissarra Susana Pereira Cláudia Pereira |
| author_facet | Tatiana Cardoso Miguel Sampaio João Neves Sofia Oliveira Inês Moura Ana Séneca José Pissarra Susana Pereira Cláudia Pereira |
| author_sort | Tatiana Cardoso |
| collection | DOAJ |
| description | The endomembrane system in plant cells enables the cell to manage and coordinate a variety of membranous compartments so that they and their contents arrive at the right location. The secretory pathway is an essential part of this complex network and has its gateway at the Endoplasmic Reticulum. Therefore, alterations at the ER can affect how protein trafficking takes place and how cargo leaves this organelle. With this work, we assessed how abnormalities at the Endoplasmic Reticulum would interfere with protein sorting and trafficking. We used an Arabidopsis mutant—<i>leb-2 GFP-h</i>, presenting abnormal ER morphology, and evaluated the expression of aspartic proteinases and genes related to vacuolar transport along with the localization of a specific vacuolar sorting signal—plant-specific insert (PSI). Our results show that alterations in the leb-2 GFP-h mutant did not disrupt the transport of PSI–mCherry to the vacuole but influenced the expression of endogenous aspartic proteinases. Furthermore, the study of key endomembrane genes expression revealed an upregulation of the SNARE proteins <i>AtVAMP722</i> and <i>AtVAMP723</i>. The leb-2 mutant seems not to interfere with vacuolar routes but may be implicated in secretion events. |
| first_indexed | 2024-03-08T20:40:34Z |
| format | Article |
| id | doaj.art-22020dccde2741a6a65724dbd0fc0bae |
| institution | Directory Open Access Journal |
| issn | 2037-0164 |
| language | English |
| last_indexed | 2024-03-08T20:40:34Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Plant Biology |
| spelling | doaj.art-22020dccde2741a6a65724dbd0fc0bae2023-12-22T14:15:32ZengMDPI AGInternational Journal of Plant Biology2037-01642023-11-011441034105010.3390/ijpb14040075Plant-Specific Insert (PSI)-Mediated Vacuolar Sorting Is Not Disrupted in Arabidopsis Mutant with Abnormal ER MorphologyTatiana Cardoso0Miguel Sampaio1João Neves2Sofia Oliveira3Inês Moura4Ana Séneca5José Pissarra6Susana Pereira7Cláudia Pereira8Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, s/n, 4169-007 Porto, PortugalDepartamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, s/n, 4169-007 Porto, PortugalDepartamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, s/n, 4169-007 Porto, PortugalDepartamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, s/n, 4169-007 Porto, PortugalDepartamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, s/n, 4169-007 Porto, PortugalDepartamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, s/n, 4169-007 Porto, PortugalDepartamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, s/n, 4169-007 Porto, PortugalDepartamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, s/n, 4169-007 Porto, PortugalDepartamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, s/n, 4169-007 Porto, PortugalThe endomembrane system in plant cells enables the cell to manage and coordinate a variety of membranous compartments so that they and their contents arrive at the right location. The secretory pathway is an essential part of this complex network and has its gateway at the Endoplasmic Reticulum. Therefore, alterations at the ER can affect how protein trafficking takes place and how cargo leaves this organelle. With this work, we assessed how abnormalities at the Endoplasmic Reticulum would interfere with protein sorting and trafficking. We used an Arabidopsis mutant—<i>leb-2 GFP-h</i>, presenting abnormal ER morphology, and evaluated the expression of aspartic proteinases and genes related to vacuolar transport along with the localization of a specific vacuolar sorting signal—plant-specific insert (PSI). Our results show that alterations in the leb-2 GFP-h mutant did not disrupt the transport of PSI–mCherry to the vacuole but influenced the expression of endogenous aspartic proteinases. Furthermore, the study of key endomembrane genes expression revealed an upregulation of the SNARE proteins <i>AtVAMP722</i> and <i>AtVAMP723</i>. The leb-2 mutant seems not to interfere with vacuolar routes but may be implicated in secretion events.https://www.mdpi.com/2037-0164/14/4/75Arabidopsisaspartic proteinasesER-bodiesendomembranesplant-specific insertvacuolar sorting |
| spellingShingle | Tatiana Cardoso Miguel Sampaio João Neves Sofia Oliveira Inês Moura Ana Séneca José Pissarra Susana Pereira Cláudia Pereira Plant-Specific Insert (PSI)-Mediated Vacuolar Sorting Is Not Disrupted in Arabidopsis Mutant with Abnormal ER Morphology International Journal of Plant Biology Arabidopsis aspartic proteinases ER-bodies endomembranes plant-specific insert vacuolar sorting |
| title | Plant-Specific Insert (PSI)-Mediated Vacuolar Sorting Is Not Disrupted in Arabidopsis Mutant with Abnormal ER Morphology |
| title_full | Plant-Specific Insert (PSI)-Mediated Vacuolar Sorting Is Not Disrupted in Arabidopsis Mutant with Abnormal ER Morphology |
| title_fullStr | Plant-Specific Insert (PSI)-Mediated Vacuolar Sorting Is Not Disrupted in Arabidopsis Mutant with Abnormal ER Morphology |
| title_full_unstemmed | Plant-Specific Insert (PSI)-Mediated Vacuolar Sorting Is Not Disrupted in Arabidopsis Mutant with Abnormal ER Morphology |
| title_short | Plant-Specific Insert (PSI)-Mediated Vacuolar Sorting Is Not Disrupted in Arabidopsis Mutant with Abnormal ER Morphology |
| title_sort | plant specific insert psi mediated vacuolar sorting is not disrupted in arabidopsis mutant with abnormal er morphology |
| topic | Arabidopsis aspartic proteinases ER-bodies endomembranes plant-specific insert vacuolar sorting |
| url | https://www.mdpi.com/2037-0164/14/4/75 |
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