Quantification and Topographical Distribution of Terminal and Linked Fucose Residues in Human Spermatozoa by Using Field Emission Scanning Electron Microscopy (FE-SEM)
The modification of sperm glycocalyx is an essential process during sperm capacitation. The presence and redistribution of terminal and linked fucose have been described during in vitro capacitation in humans. However, the influence of the capacitation time on the quantification and localization of...
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MDPI AG
2021-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/21/11947 |
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| author | Laura Robles-Gómez Paula Sáez-Espinosa Eliana Marina López-Viloria Andrea López-Botella Jon Aizpurua María José Gómez-Torres |
| author_facet | Laura Robles-Gómez Paula Sáez-Espinosa Eliana Marina López-Viloria Andrea López-Botella Jon Aizpurua María José Gómez-Torres |
| author_sort | Laura Robles-Gómez |
| collection | DOAJ |
| description | The modification of sperm glycocalyx is an essential process during sperm capacitation. The presence and redistribution of terminal and linked fucose have been described during in vitro capacitation in humans. However, the influence of the capacitation time on the quantification and localization of terminal and linked fucose is still unknown. In this study, the quantitative and qualitative changes in fucosyl residues during different in vitro capacitation times (1 and 4 h), are simultaneously characterized by using <i>Aleuria aurantia</i> (AAA) lectin–gold labelling and high-resolution field emission scanning electron microscopy (FE-SEM) in human sperm. A significant decrease was found in the number of terminal fucose registered in the whole sperm head during the in vitro capacitation. Nevertheless, the quantification of fucose residues after 1 h of in vitro capacitation was very similar to those found after 4 h. Therefore, the changes observed in terminal and linked fucose during capacitation were not time-dependent. Furthermore, the comprehensive analysis of the topographic distribution showed the preferential fucosyl location in the acrosomal region and the presence of distinct clusters distributed over the head in all the studied conditions. Overall, these findings corroborate the validity of FE-SEM combined with gold labelling to register changes in surface molecules during in vitro sperm capacitation. |
| first_indexed | 2024-03-10T06:00:24Z |
| format | Article |
| id | doaj.art-2205fd936f90466993c41b7bdfacc835 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T06:00:24Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2205fd936f90466993c41b7bdfacc8352023-11-22T21:00:21ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-11-0122211194710.3390/ijms222111947Quantification and Topographical Distribution of Terminal and Linked Fucose Residues in Human Spermatozoa by Using Field Emission Scanning Electron Microscopy (FE-SEM)Laura Robles-Gómez0Paula Sáez-Espinosa1Eliana Marina López-Viloria2Andrea López-Botella3Jon Aizpurua4María José Gómez-Torres5Departamento de Biotecnología, Universidad de Alicante, 03080 Alicante, SpainDepartamento de Biotecnología, Universidad de Alicante, 03080 Alicante, SpainDepartamento de Biotecnología, Universidad de Alicante, 03080 Alicante, SpainDepartamento de Biotecnología, Universidad de Alicante, 03080 Alicante, SpainIVF Spain, Reproductive Medicine, 03540 Alicante, SpainDepartamento de Biotecnología, Universidad de Alicante, 03080 Alicante, SpainThe modification of sperm glycocalyx is an essential process during sperm capacitation. The presence and redistribution of terminal and linked fucose have been described during in vitro capacitation in humans. However, the influence of the capacitation time on the quantification and localization of terminal and linked fucose is still unknown. In this study, the quantitative and qualitative changes in fucosyl residues during different in vitro capacitation times (1 and 4 h), are simultaneously characterized by using <i>Aleuria aurantia</i> (AAA) lectin–gold labelling and high-resolution field emission scanning electron microscopy (FE-SEM) in human sperm. A significant decrease was found in the number of terminal fucose registered in the whole sperm head during the in vitro capacitation. Nevertheless, the quantification of fucose residues after 1 h of in vitro capacitation was very similar to those found after 4 h. Therefore, the changes observed in terminal and linked fucose during capacitation were not time-dependent. Furthermore, the comprehensive analysis of the topographic distribution showed the preferential fucosyl location in the acrosomal region and the presence of distinct clusters distributed over the head in all the studied conditions. Overall, these findings corroborate the validity of FE-SEM combined with gold labelling to register changes in surface molecules during in vitro sperm capacitation.https://www.mdpi.com/1422-0067/22/21/11947<i>Aleuria aurantia</i> lectincapacitationFE-SEMfucoseglycoconjugatessperm |
| spellingShingle | Laura Robles-Gómez Paula Sáez-Espinosa Eliana Marina López-Viloria Andrea López-Botella Jon Aizpurua María José Gómez-Torres Quantification and Topographical Distribution of Terminal and Linked Fucose Residues in Human Spermatozoa by Using Field Emission Scanning Electron Microscopy (FE-SEM) International Journal of Molecular Sciences <i>Aleuria aurantia</i> lectin capacitation FE-SEM fucose glycoconjugates sperm |
| title | Quantification and Topographical Distribution of Terminal and Linked Fucose Residues in Human Spermatozoa by Using Field Emission Scanning Electron Microscopy (FE-SEM) |
| title_full | Quantification and Topographical Distribution of Terminal and Linked Fucose Residues in Human Spermatozoa by Using Field Emission Scanning Electron Microscopy (FE-SEM) |
| title_fullStr | Quantification and Topographical Distribution of Terminal and Linked Fucose Residues in Human Spermatozoa by Using Field Emission Scanning Electron Microscopy (FE-SEM) |
| title_full_unstemmed | Quantification and Topographical Distribution of Terminal and Linked Fucose Residues in Human Spermatozoa by Using Field Emission Scanning Electron Microscopy (FE-SEM) |
| title_short | Quantification and Topographical Distribution of Terminal and Linked Fucose Residues in Human Spermatozoa by Using Field Emission Scanning Electron Microscopy (FE-SEM) |
| title_sort | quantification and topographical distribution of terminal and linked fucose residues in human spermatozoa by using field emission scanning electron microscopy fe sem |
| topic | <i>Aleuria aurantia</i> lectin capacitation FE-SEM fucose glycoconjugates sperm |
| url | https://www.mdpi.com/1422-0067/22/21/11947 |
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