Detection of SNPs in FABP4 Gene and Its Relationship with Milk Quality Traits in Iraqi Jenoubi Cows
Jenoubi cattle breed classified as a Bos indicus L., is one of Iraqi indigenous cattle that kept for milk production purpose, but the influence of fatty acid-binding protein 4 (FABP4) on milk-related traits remain poorly understood. The current study was conducted to investigate the effect of single...
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| Format: | Article |
| Language: | English |
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University of Basrah
2023-06-01
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| Series: | Maǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ |
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| Online Access: | https://bjas.bajas.edu.iq/index.php/bjas/article/view/912 |
| _version_ | 1797779174527598592 |
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| author | Salah H. Faraj Widya P. B. Putra Thobela L. Tyasi |
| author_facet | Salah H. Faraj Widya P. B. Putra Thobela L. Tyasi |
| author_sort | Salah H. Faraj |
| collection | DOAJ |
| description | Jenoubi cattle breed classified as a Bos indicus L., is one of Iraqi indigenous cattle that kept for milk production purpose, but the influence of fatty acid-binding protein 4 (FABP4) on milk-related traits remain poorly understood. The current study was conducted to investigate the effect of single nucleotide polymorphisms (SNPs) in the FABP4 gene of Jenoubi cows. The DNA samples were extracted from the blood of 21 cows. Along 565-bp nucleotide (nt) amplicon from the intron 2- exon 3- intron 3 of FABP4 was amplified by polymerase chain reaction (PCR) while forward sequencing was used to detect SNPs and results were analyzed using bioinformatics software. The findings resulted the existence of two SNPs (c.3689G>A and c.3709G>C) in the exon 3 and four SNPs (g.3494A>C, g.3531A>T, g.3743T>C and g.3765T>C) in the intron regions. Therefore, three haplotypes of FABP4 gene were obtained in this study based on three SNPs of g.3494A>C, g.3531A>T and g.3765T>C. Nonetheless, those haplotypes were not significantly influencing to the milk quality traits of Jenoubi cows. However, the SNPs examined in this study might not be used as potential DNA marker to improve milk production traits of the current breed.
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| first_indexed | 2024-03-12T23:27:52Z |
| format | Article |
| id | doaj.art-2206c4bc9c34414a88b5f0d29f675ed0 |
| institution | Directory Open Access Journal |
| issn | 1814-5868 2520-0860 |
| language | English |
| last_indexed | 2024-03-12T23:27:52Z |
| publishDate | 2023-06-01 |
| publisher | University of Basrah |
| record_format | Article |
| series | Maǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ |
| spelling | doaj.art-2206c4bc9c34414a88b5f0d29f675ed02023-07-15T17:36:15ZengUniversity of BasrahMaǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ1814-58682520-08602023-06-01361Detection of SNPs in FABP4 Gene and Its Relationship with Milk Quality Traits in Iraqi Jenoubi CowsSalah H. Faraj0Widya P. B. Putra1Thobela L. Tyasi2Department of Biology, College of Science, University of Misan, Maysan, IraqResearch Center for Applied Zoology, National Research and Innovation Agency, Bogor, West Java, Indonesia 16911Department of Agricultural Economics and Animal Production, School of Agricultural and Environmental Sciences, University of Limpopo, Sovenga, South Africa 0727Jenoubi cattle breed classified as a Bos indicus L., is one of Iraqi indigenous cattle that kept for milk production purpose, but the influence of fatty acid-binding protein 4 (FABP4) on milk-related traits remain poorly understood. The current study was conducted to investigate the effect of single nucleotide polymorphisms (SNPs) in the FABP4 gene of Jenoubi cows. The DNA samples were extracted from the blood of 21 cows. Along 565-bp nucleotide (nt) amplicon from the intron 2- exon 3- intron 3 of FABP4 was amplified by polymerase chain reaction (PCR) while forward sequencing was used to detect SNPs and results were analyzed using bioinformatics software. The findings resulted the existence of two SNPs (c.3689G>A and c.3709G>C) in the exon 3 and four SNPs (g.3494A>C, g.3531A>T, g.3743T>C and g.3765T>C) in the intron regions. Therefore, three haplotypes of FABP4 gene were obtained in this study based on three SNPs of g.3494A>C, g.3531A>T and g.3765T>C. Nonetheless, those haplotypes were not significantly influencing to the milk quality traits of Jenoubi cows. However, the SNPs examined in this study might not be used as potential DNA marker to improve milk production traits of the current breed. https://bjas.bajas.edu.iq/index.php/bjas/article/view/912FABP4 geneIraqi Jenoubi CowsMilk yieldSingle nucleotide polymorphism |
| spellingShingle | Salah H. Faraj Widya P. B. Putra Thobela L. Tyasi Detection of SNPs in FABP4 Gene and Its Relationship with Milk Quality Traits in Iraqi Jenoubi Cows Maǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ FABP4 gene Iraqi Jenoubi Cows Milk yield Single nucleotide polymorphism |
| title | Detection of SNPs in FABP4 Gene and Its Relationship with Milk Quality Traits in Iraqi Jenoubi Cows |
| title_full | Detection of SNPs in FABP4 Gene and Its Relationship with Milk Quality Traits in Iraqi Jenoubi Cows |
| title_fullStr | Detection of SNPs in FABP4 Gene and Its Relationship with Milk Quality Traits in Iraqi Jenoubi Cows |
| title_full_unstemmed | Detection of SNPs in FABP4 Gene and Its Relationship with Milk Quality Traits in Iraqi Jenoubi Cows |
| title_short | Detection of SNPs in FABP4 Gene and Its Relationship with Milk Quality Traits in Iraqi Jenoubi Cows |
| title_sort | detection of snps in fabp4 gene and its relationship with milk quality traits in iraqi jenoubi cows |
| topic | FABP4 gene Iraqi Jenoubi Cows Milk yield Single nucleotide polymorphism |
| url | https://bjas.bajas.edu.iq/index.php/bjas/article/view/912 |
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