Immune Profiling of Cord Blood From Preterm and Term Infants Reveals Distinct Differences in Pro-Inflammatory Responses
BackgroundPreterm infants are highly vulnerable to infectious disease. While many factors are likely to contribute to this enhanced susceptibility, the immature nature of the preterm immune system is postulated as one key factor.MethodsIn our study, we used high-dimensional flow cytometry and cytoki...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.777927/full |
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| author | Jeremy Anderson Jeremy Anderson Cao Minh Thang Le Quang Thanh Vo Thi Trang Dai Van Thanh Phan Bui Thi Hong Nhu Do Ngoc Xuan Trang Phan Thi Phuong Trinh Thuong Vu Nguyen Nguyen Trong Toan Christopher M. Harpur Kim Mulholland Kim Mulholland Kim Mulholland Daniel G. Pellicci Daniel G. Pellicci Daniel G. Pellicci Lien Anh Ha Do Lien Anh Ha Do Paul V. Licciardi Paul V. Licciardi |
| author_facet | Jeremy Anderson Jeremy Anderson Cao Minh Thang Le Quang Thanh Vo Thi Trang Dai Van Thanh Phan Bui Thi Hong Nhu Do Ngoc Xuan Trang Phan Thi Phuong Trinh Thuong Vu Nguyen Nguyen Trong Toan Christopher M. Harpur Kim Mulholland Kim Mulholland Kim Mulholland Daniel G. Pellicci Daniel G. Pellicci Daniel G. Pellicci Lien Anh Ha Do Lien Anh Ha Do Paul V. Licciardi Paul V. Licciardi |
| author_sort | Jeremy Anderson |
| collection | DOAJ |
| description | BackgroundPreterm infants are highly vulnerable to infectious disease. While many factors are likely to contribute to this enhanced susceptibility, the immature nature of the preterm immune system is postulated as one key factor.MethodsIn our study, we used high-dimensional flow cytometry and cytokine assays to characterise the immune profiles in 25 preterm (range: 30.4-34.1 weeks gestational age) and 25 term infant (range: 37-40 weeks gestational age) cord blood samples.ResultsWe found that preterm infants exhibit reduced frequencies of monocytes, CD56bright NK cells, CD8+ T-cells, γδ T-cells and an increased frequency of intermediate monocytes, CD4+ T-cells, central memory CD4+ and CD8+ T-cells, Tregs and transitional B-cells compared to term infants. Pro-inflammatory cytokines IL-1β, IL-6 and IL-17A were lower in preterm infants in addition to chemokines IL-8, eotaxin, MIP-1α and MIP-1β. However, IL-15 and MCP-1 were higher in preterm infants.ConclusionOverall, we identify key differences in pro-inflammatory immune profiles between preterm and term infants. These findings may help to explain why preterm infants are more susceptible to infectious disease during early life and facilitate the development of targeted interventions to protect this highly vulnerable group. |
| first_indexed | 2024-12-16T10:20:37Z |
| format | Article |
| id | doaj.art-2208e3ad0a024157bc49c665f611b1bf |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-16T10:20:37Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-2208e3ad0a024157bc49c665f611b1bf2022-12-21T22:35:19ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-11-011210.3389/fimmu.2021.777927777927Immune Profiling of Cord Blood From Preterm and Term Infants Reveals Distinct Differences in Pro-Inflammatory ResponsesJeremy Anderson0Jeremy Anderson1Cao Minh Thang2Le Quang Thanh3Vo Thi Trang Dai4Van Thanh Phan5Bui Thi Hong Nhu6Do Ngoc Xuan Trang7Phan Thi Phuong Trinh8Thuong Vu Nguyen9Nguyen Trong Toan10Christopher M. Harpur11Kim Mulholland12Kim Mulholland13Kim Mulholland14Daniel G. Pellicci15Daniel G. Pellicci16Daniel G. Pellicci17Lien Anh Ha Do18Lien Anh Ha Do19Paul V. Licciardi20Paul V. Licciardi21Infection and Immunity, Murdoch Children’s Research Institute, Melbourne, VIC, AustraliaDepartment of Paediatrics, University of Melbourne, Melbourne, VIC, AustraliaDepartment of Microbiology and Immunology, Pasteur Institute of Ho Chi Minh City, Ho Chi Minh, VietnamTu Du Hospital, Ho Chi Minh, VietnamDepartment of Microbiology and Immunology, Pasteur Institute of Ho Chi Minh City, Ho Chi Minh, VietnamDepartment of Microbiology and Immunology, Pasteur Institute of Ho Chi Minh City, Ho Chi Minh, VietnamDepartment of Labour Delivery, Tu Du Hospital, Ho Chi Minh, VietnamDepartment of Labour Delivery, Tu Du Hospital, Ho Chi Minh, VietnamDepartment of Labour Delivery, Tu Du Hospital, Ho Chi Minh, VietnamPasteur Institute of Ho Chi Minh City, Ho Chi Minh, VietnamClinical Research Centre, Pasteur Institute of Ho Chi Minh City, Ho Chi Minh, VietnamInfection and Immunity, Murdoch Children’s Research Institute, Melbourne, VIC, AustraliaInfection and Immunity, Murdoch Children’s Research Institute, Melbourne, VIC, AustraliaDepartment of Paediatrics, University of Melbourne, Melbourne, VIC, AustraliaEpidemiology and Public Health, London School of Hygiene and Tropical Medicine, London, United KingdomInfection and Immunity, Murdoch Children’s Research Institute, Melbourne, VIC, AustraliaDepartment of Paediatrics, University of Melbourne, Melbourne, VIC, AustraliaDepartment of Microbiology and Immunology, University of Melbourne, Melbourne, VIC, AustraliaInfection and Immunity, Murdoch Children’s Research Institute, Melbourne, VIC, AustraliaDepartment of Paediatrics, University of Melbourne, Melbourne, VIC, AustraliaInfection and Immunity, Murdoch Children’s Research Institute, Melbourne, VIC, AustraliaDepartment of Paediatrics, University of Melbourne, Melbourne, VIC, AustraliaBackgroundPreterm infants are highly vulnerable to infectious disease. While many factors are likely to contribute to this enhanced susceptibility, the immature nature of the preterm immune system is postulated as one key factor.MethodsIn our study, we used high-dimensional flow cytometry and cytokine assays to characterise the immune profiles in 25 preterm (range: 30.4-34.1 weeks gestational age) and 25 term infant (range: 37-40 weeks gestational age) cord blood samples.ResultsWe found that preterm infants exhibit reduced frequencies of monocytes, CD56bright NK cells, CD8+ T-cells, γδ T-cells and an increased frequency of intermediate monocytes, CD4+ T-cells, central memory CD4+ and CD8+ T-cells, Tregs and transitional B-cells compared to term infants. Pro-inflammatory cytokines IL-1β, IL-6 and IL-17A were lower in preterm infants in addition to chemokines IL-8, eotaxin, MIP-1α and MIP-1β. However, IL-15 and MCP-1 were higher in preterm infants.ConclusionOverall, we identify key differences in pro-inflammatory immune profiles between preterm and term infants. These findings may help to explain why preterm infants are more susceptible to infectious disease during early life and facilitate the development of targeted interventions to protect this highly vulnerable group.https://www.frontiersin.org/articles/10.3389/fimmu.2021.777927/fullpreterminfantimmune profileinfectioninflammation |
| spellingShingle | Jeremy Anderson Jeremy Anderson Cao Minh Thang Le Quang Thanh Vo Thi Trang Dai Van Thanh Phan Bui Thi Hong Nhu Do Ngoc Xuan Trang Phan Thi Phuong Trinh Thuong Vu Nguyen Nguyen Trong Toan Christopher M. Harpur Kim Mulholland Kim Mulholland Kim Mulholland Daniel G. Pellicci Daniel G. Pellicci Daniel G. Pellicci Lien Anh Ha Do Lien Anh Ha Do Paul V. Licciardi Paul V. Licciardi Immune Profiling of Cord Blood From Preterm and Term Infants Reveals Distinct Differences in Pro-Inflammatory Responses Frontiers in Immunology preterm infant immune profile infection inflammation |
| title | Immune Profiling of Cord Blood From Preterm and Term Infants Reveals Distinct Differences in Pro-Inflammatory Responses |
| title_full | Immune Profiling of Cord Blood From Preterm and Term Infants Reveals Distinct Differences in Pro-Inflammatory Responses |
| title_fullStr | Immune Profiling of Cord Blood From Preterm and Term Infants Reveals Distinct Differences in Pro-Inflammatory Responses |
| title_full_unstemmed | Immune Profiling of Cord Blood From Preterm and Term Infants Reveals Distinct Differences in Pro-Inflammatory Responses |
| title_short | Immune Profiling of Cord Blood From Preterm and Term Infants Reveals Distinct Differences in Pro-Inflammatory Responses |
| title_sort | immune profiling of cord blood from preterm and term infants reveals distinct differences in pro inflammatory responses |
| topic | preterm infant immune profile infection inflammation |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2021.777927/full |
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