Host Long Noncoding RNA lncRNA-PAAN Regulates the Replication of Influenza A Virus
The productive infection of influenza A virus (IAV) depends on host factors. However, the involvement of long non-coding RNAs (lncRNAs) in IAV infection remains largely uninvestigated. In this work, we have discovered a human lncRNA, named lncRNA-PAAN (PA-associated noncoding RNA) that enhances IAV...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-06-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1999-4915/10/6/330 |
| _version_ | 1819073383916109824 |
|---|---|
| author | Jing Wang Yujia Wang Rui Zhou Jianyuan Zhao Yongxin Zhang Dongrong Yi Quanjie Li Jinming Zhou Fei Guo Chen Liang Xiaoyu Li Shan Cen |
| author_facet | Jing Wang Yujia Wang Rui Zhou Jianyuan Zhao Yongxin Zhang Dongrong Yi Quanjie Li Jinming Zhou Fei Guo Chen Liang Xiaoyu Li Shan Cen |
| author_sort | Jing Wang |
| collection | DOAJ |
| description | The productive infection of influenza A virus (IAV) depends on host factors. However, the involvement of long non-coding RNAs (lncRNAs) in IAV infection remains largely uninvestigated. In this work, we have discovered a human lncRNA, named lncRNA-PAAN (PA-associated noncoding RNA) that enhances IAV replication. The level of lncRNA-PAAN increases upon infection of IAV, but not other viruses, nor interferon treatment, suggesting specific up-regulation of lncRNA-PAAN expression by IAV. Silencing lncRNA-PAAN significantly decreases IAV replication through impairing the activity of viral RNA-dependent RNA polymerase (RdRp). This function of lncRNA-PAAN is a result of its association with viral PA protein, a key component of IAV RNA polymerase complex. Consequently, depletion of lncRNA-PAAN prevents the formation of functional RdRp. Together, these results suggest that lncRNA-PAAN promotes the assembly of viral RNA polymerase, thus warranting efficient viral RNA synthesis. Elucidating the functions of lncRNAs in IAV infection is expected to advance our understanding of IAV pathogenesis and open new avenues to the development of novel anti-IAV therapeutics. |
| first_indexed | 2024-12-21T17:52:45Z |
| format | Article |
| id | doaj.art-220bcbb3d202480c816243b3db95f0f5 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-21T17:52:45Z |
| publishDate | 2018-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-220bcbb3d202480c816243b3db95f0f52022-12-21T18:55:17ZengMDPI AGViruses1999-49152018-06-0110633010.3390/v10060330v10060330Host Long Noncoding RNA lncRNA-PAAN Regulates the Replication of Influenza A VirusJing Wang0Yujia Wang1Rui Zhou2Jianyuan Zhao3Yongxin Zhang4Dongrong Yi5Quanjie Li6Jinming Zhou7Fei Guo8Chen Liang9Xiaoyu Li10Shan Cen11Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical School, Beijing 100050, ChinaInstitute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical School, Beijing 100050, ChinaInstitute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical School, Beijing 100050, ChinaInstitute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical School, Beijing 100050, ChinaInstitute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical School, Beijing 100050, ChinaInstitute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical School, Beijing 100050, ChinaInstitute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical School, Beijing 100050, ChinaInstitute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical School, Beijing 100050, ChinaInstitute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical School, Beijing 100730, ChinaLady Davis Institute for Medical Research, Jewish General Hospital, McGill University, Montreal, QC H3T 1E2, CanadaInstitute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical School, Beijing 100050, ChinaInstitute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical School, Beijing 100050, ChinaThe productive infection of influenza A virus (IAV) depends on host factors. However, the involvement of long non-coding RNAs (lncRNAs) in IAV infection remains largely uninvestigated. In this work, we have discovered a human lncRNA, named lncRNA-PAAN (PA-associated noncoding RNA) that enhances IAV replication. The level of lncRNA-PAAN increases upon infection of IAV, but not other viruses, nor interferon treatment, suggesting specific up-regulation of lncRNA-PAAN expression by IAV. Silencing lncRNA-PAAN significantly decreases IAV replication through impairing the activity of viral RNA-dependent RNA polymerase (RdRp). This function of lncRNA-PAAN is a result of its association with viral PA protein, a key component of IAV RNA polymerase complex. Consequently, depletion of lncRNA-PAAN prevents the formation of functional RdRp. Together, these results suggest that lncRNA-PAAN promotes the assembly of viral RNA polymerase, thus warranting efficient viral RNA synthesis. Elucidating the functions of lncRNAs in IAV infection is expected to advance our understanding of IAV pathogenesis and open new avenues to the development of novel anti-IAV therapeutics.http://www.mdpi.com/1999-4915/10/6/330lncRNAinfluenza A virusPAANpolymerase basic protein 1 (PB1)polymerase acidic protein (PA)RdRp |
| spellingShingle | Jing Wang Yujia Wang Rui Zhou Jianyuan Zhao Yongxin Zhang Dongrong Yi Quanjie Li Jinming Zhou Fei Guo Chen Liang Xiaoyu Li Shan Cen Host Long Noncoding RNA lncRNA-PAAN Regulates the Replication of Influenza A Virus Viruses lncRNA influenza A virus PAAN polymerase basic protein 1 (PB1) polymerase acidic protein (PA) RdRp |
| title | Host Long Noncoding RNA lncRNA-PAAN Regulates the Replication of Influenza A Virus |
| title_full | Host Long Noncoding RNA lncRNA-PAAN Regulates the Replication of Influenza A Virus |
| title_fullStr | Host Long Noncoding RNA lncRNA-PAAN Regulates the Replication of Influenza A Virus |
| title_full_unstemmed | Host Long Noncoding RNA lncRNA-PAAN Regulates the Replication of Influenza A Virus |
| title_short | Host Long Noncoding RNA lncRNA-PAAN Regulates the Replication of Influenza A Virus |
| title_sort | host long noncoding rna lncrna paan regulates the replication of influenza a virus |
| topic | lncRNA influenza A virus PAAN polymerase basic protein 1 (PB1) polymerase acidic protein (PA) RdRp |
| url | http://www.mdpi.com/1999-4915/10/6/330 |
| work_keys_str_mv | AT jingwang hostlongnoncodingrnalncrnapaanregulatesthereplicationofinfluenzaavirus AT yujiawang hostlongnoncodingrnalncrnapaanregulatesthereplicationofinfluenzaavirus AT ruizhou hostlongnoncodingrnalncrnapaanregulatesthereplicationofinfluenzaavirus AT jianyuanzhao hostlongnoncodingrnalncrnapaanregulatesthereplicationofinfluenzaavirus AT yongxinzhang hostlongnoncodingrnalncrnapaanregulatesthereplicationofinfluenzaavirus AT dongrongyi hostlongnoncodingrnalncrnapaanregulatesthereplicationofinfluenzaavirus AT quanjieli hostlongnoncodingrnalncrnapaanregulatesthereplicationofinfluenzaavirus AT jinmingzhou hostlongnoncodingrnalncrnapaanregulatesthereplicationofinfluenzaavirus AT feiguo hostlongnoncodingrnalncrnapaanregulatesthereplicationofinfluenzaavirus AT chenliang hostlongnoncodingrnalncrnapaanregulatesthereplicationofinfluenzaavirus AT xiaoyuli hostlongnoncodingrnalncrnapaanregulatesthereplicationofinfluenzaavirus AT shancen hostlongnoncodingrnalncrnapaanregulatesthereplicationofinfluenzaavirus |