bta-miR-23a Regulates the Myogenic Differentiation of Fetal Bovine Skeletal Muscle-Derived Progenitor Cells by Targeting <i>MDFIC</i> Gene
miR-23a, a member of the miR-23a/24-2/27a cluster, has been demonstrated to play pivotal roles in many cellular activities. However, the mechanisms of how bta-miR-23a controls the myogenic differentiation (MD) of PDGFRα<sup>−</sup> bovine progenitor cells (bPCs) remain poorly understood....
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MDPI AG
2020-10-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/11/10/1232 |
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| author | Xin Hu Yishen Xing Ling Ren Yahui Wang Qian Li Qiyuan Yang Min Du Lingyang Xu Luc Willems Junya Li Lupei Zhang |
| author_facet | Xin Hu Yishen Xing Ling Ren Yahui Wang Qian Li Qiyuan Yang Min Du Lingyang Xu Luc Willems Junya Li Lupei Zhang |
| author_sort | Xin Hu |
| collection | DOAJ |
| description | miR-23a, a member of the miR-23a/24-2/27a cluster, has been demonstrated to play pivotal roles in many cellular activities. However, the mechanisms of how bta-miR-23a controls the myogenic differentiation (MD) of PDGFRα<sup>−</sup> bovine progenitor cells (bPCs) remain poorly understood. In the present work, bta-miR-23a expression was increased during the MD of <sup>PDGFRα−</sup> bPCs. Moreover, bta-miR-23a overexpression significantly promoted the MD of <sup>PDGFRα−</sup> bPCs. Luciferase reporter assays showed that the 3’-UTR region of <i>MDFIC</i> (MyoD family inhibitor domain containing) could be a promising target of bta-miR-23a, which resulted in its post-transcriptional down-regulation. Additionally, the knockdown of <i>MDFIC</i> by siRNA facilitated the MD of <sup>PDGFRα−</sup> bPCs, while the overexpression of <i>MDFIC</i> inhibited the activating effect of bta-miR-23a during MD. Of note, <i>MDFIC</i> might function through the interaction between <i>MyoG</i> transcription factor and <i>MEF2C</i> promoter. This study reveals that bta-miR-23a can promote the MD of <sup>PDGFRα−</sup> bPCs through post-transcriptional downregulation of <i>MDFIC</i>. |
| first_indexed | 2024-03-10T15:28:20Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T15:28:20Z |
| publishDate | 2020-10-01 |
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| record_format | Article |
| series | Genes |
| spelling | doaj.art-220e5abc410c4cc4b6a038def039df3f2023-11-20T17:50:10ZengMDPI AGGenes2073-44252020-10-011110123210.3390/genes11101232bta-miR-23a Regulates the Myogenic Differentiation of Fetal Bovine Skeletal Muscle-Derived Progenitor Cells by Targeting <i>MDFIC</i> GeneXin Hu0Yishen Xing1Ling Ren2Yahui Wang3Qian Li4Qiyuan Yang5Min Du6Lingyang Xu7Luc Willems8Junya Li9Lupei Zhang10Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaDepartment of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, MA 01655, USAWashington Center for Muscle Biology and Department of Animal Sciences, Washington State University, Pullman, WA 99164, USAInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaMolecular and Cellular Biology, Gembloux Agro-Bio Tech, University of Liège, 5030 Gembloux, BelgiumInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinamiR-23a, a member of the miR-23a/24-2/27a cluster, has been demonstrated to play pivotal roles in many cellular activities. However, the mechanisms of how bta-miR-23a controls the myogenic differentiation (MD) of PDGFRα<sup>−</sup> bovine progenitor cells (bPCs) remain poorly understood. In the present work, bta-miR-23a expression was increased during the MD of <sup>PDGFRα−</sup> bPCs. Moreover, bta-miR-23a overexpression significantly promoted the MD of <sup>PDGFRα−</sup> bPCs. Luciferase reporter assays showed that the 3’-UTR region of <i>MDFIC</i> (MyoD family inhibitor domain containing) could be a promising target of bta-miR-23a, which resulted in its post-transcriptional down-regulation. Additionally, the knockdown of <i>MDFIC</i> by siRNA facilitated the MD of <sup>PDGFRα−</sup> bPCs, while the overexpression of <i>MDFIC</i> inhibited the activating effect of bta-miR-23a during MD. Of note, <i>MDFIC</i> might function through the interaction between <i>MyoG</i> transcription factor and <i>MEF2C</i> promoter. This study reveals that bta-miR-23a can promote the MD of <sup>PDGFRα−</sup> bPCs through post-transcriptional downregulation of <i>MDFIC</i>.https://www.mdpi.com/2073-4425/11/10/1232bta-miR-23afetal muscle development<i>MDFIC</i> |
| spellingShingle | Xin Hu Yishen Xing Ling Ren Yahui Wang Qian Li Qiyuan Yang Min Du Lingyang Xu Luc Willems Junya Li Lupei Zhang bta-miR-23a Regulates the Myogenic Differentiation of Fetal Bovine Skeletal Muscle-Derived Progenitor Cells by Targeting <i>MDFIC</i> Gene Genes bta-miR-23a fetal muscle development <i>MDFIC</i> |
| title | bta-miR-23a Regulates the Myogenic Differentiation of Fetal Bovine Skeletal Muscle-Derived Progenitor Cells by Targeting <i>MDFIC</i> Gene |
| title_full | bta-miR-23a Regulates the Myogenic Differentiation of Fetal Bovine Skeletal Muscle-Derived Progenitor Cells by Targeting <i>MDFIC</i> Gene |
| title_fullStr | bta-miR-23a Regulates the Myogenic Differentiation of Fetal Bovine Skeletal Muscle-Derived Progenitor Cells by Targeting <i>MDFIC</i> Gene |
| title_full_unstemmed | bta-miR-23a Regulates the Myogenic Differentiation of Fetal Bovine Skeletal Muscle-Derived Progenitor Cells by Targeting <i>MDFIC</i> Gene |
| title_short | bta-miR-23a Regulates the Myogenic Differentiation of Fetal Bovine Skeletal Muscle-Derived Progenitor Cells by Targeting <i>MDFIC</i> Gene |
| title_sort | bta mir 23a regulates the myogenic differentiation of fetal bovine skeletal muscle derived progenitor cells by targeting i mdfic i gene |
| topic | bta-miR-23a fetal muscle development <i>MDFIC</i> |
| url | https://www.mdpi.com/2073-4425/11/10/1232 |
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