Extraction of Polyphenols from Olive Leaves Employing Deep Eutectic Solvents: The Application of Chemometrics to a Quantitative Study on Antioxidant Compounds
The extraction of phenolic compounds from olive leaves was optimized using three glycerol-based deep eutectic solvents (DESs) with lysine, proline, and arginine. A three-level Box–Behnken design was used to examine the influence of the liquid/solid ratio, concentration of DESs, and extraction temper...
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MDPI AG
2022-01-01
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| author | Hamida Akli Spyros Grigorakis Abdessamie Kellil Sofia Loupassaki Dimitris P. Makris Antony Calokerinos Abderrahmane Mati Nikos Lydakis-Simantiris |
| author_facet | Hamida Akli Spyros Grigorakis Abdessamie Kellil Sofia Loupassaki Dimitris P. Makris Antony Calokerinos Abderrahmane Mati Nikos Lydakis-Simantiris |
| author_sort | Hamida Akli |
| collection | DOAJ |
| description | The extraction of phenolic compounds from olive leaves was optimized using three glycerol-based deep eutectic solvents (DESs) with lysine, proline, and arginine. A three-level Box–Behnken design was used to examine the influence of the liquid/solid ratio, concentration of DESs, and extraction temperature on the yield of the extraction process. A second-order polynomial model was used for predicting the polyphenol extraction yield. The optimal predicted conditions were used for extractions and they provided the highest total phenol yields with the glycerol–lysine exhibiting the best performance. Quantification of tyrosol, hydroxytyrosol, oleuropein, luteolin-7-O-glucoside, and rutin in the extracts showed high content in tyrosol in all DESs, particularly with glycerol–lysine and relatively similar contents with other studies for the other phenolic compounds. Finally, a linear relationship between tyrosol content and the total phenolic content of the extracts was observed. |
| first_indexed | 2024-03-10T01:57:34Z |
| format | Article |
| id | doaj.art-220f19d2cd614eb89ce8db0df334bed9 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T01:57:34Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-220f19d2cd614eb89ce8db0df334bed92023-11-23T12:53:34ZengMDPI AGApplied Sciences2076-34172022-01-0112283110.3390/app12020831Extraction of Polyphenols from Olive Leaves Employing Deep Eutectic Solvents: The Application of Chemometrics to a Quantitative Study on Antioxidant CompoundsHamida Akli0Spyros Grigorakis1Abdessamie Kellil2Sofia Loupassaki3Dimitris P. Makris4Antony Calokerinos5Abderrahmane Mati6Nikos Lydakis-Simantiris7Food Quality & Chemistry of Natural Products, Mediterranean Agronomic Institute of Chania, International Centre for Advanced Mediterranean Agronomic Studies, P.O. Box 85, 73100 Chania, GreeceFood Quality & Chemistry of Natural Products, Mediterranean Agronomic Institute of Chania, International Centre for Advanced Mediterranean Agronomic Studies, P.O. Box 85, 73100 Chania, GreeceFood Quality & Chemistry of Natural Products, Mediterranean Agronomic Institute of Chania, International Centre for Advanced Mediterranean Agronomic Studies, P.O. Box 85, 73100 Chania, GreeceFood Quality & Chemistry of Natural Products, Mediterranean Agronomic Institute of Chania, International Centre for Advanced Mediterranean Agronomic Studies, P.O. Box 85, 73100 Chania, GreeceGreen Processes & Biorefinery Group, School of Agricultural Sciences, University of Thessaly, N. Temponera Street, 43100 Karditsa, GreeceDepartment of Chemistry, National and Kapodistrian University of Athens, 15772 Athens, GreeceAnalytical Biochemistry and Biotechnologies Research Laboratory (LABAB), Mouloud Mammeri University, Tizi Ouzou 15000, AlgeriaHellenic Mediterranean University Research Center, Institute of Agri-Food and Life Sciences, 71410 Heraklion, GreeceThe extraction of phenolic compounds from olive leaves was optimized using three glycerol-based deep eutectic solvents (DESs) with lysine, proline, and arginine. A three-level Box–Behnken design was used to examine the influence of the liquid/solid ratio, concentration of DESs, and extraction temperature on the yield of the extraction process. A second-order polynomial model was used for predicting the polyphenol extraction yield. The optimal predicted conditions were used for extractions and they provided the highest total phenol yields with the glycerol–lysine exhibiting the best performance. Quantification of tyrosol, hydroxytyrosol, oleuropein, luteolin-7-O-glucoside, and rutin in the extracts showed high content in tyrosol in all DESs, particularly with glycerol–lysine and relatively similar contents with other studies for the other phenolic compounds. Finally, a linear relationship between tyrosol content and the total phenolic content of the extracts was observed.https://www.mdpi.com/2076-3417/12/2/831deep eutectic solventsextractionpolyphenolsolive leaves |
| spellingShingle | Hamida Akli Spyros Grigorakis Abdessamie Kellil Sofia Loupassaki Dimitris P. Makris Antony Calokerinos Abderrahmane Mati Nikos Lydakis-Simantiris Extraction of Polyphenols from Olive Leaves Employing Deep Eutectic Solvents: The Application of Chemometrics to a Quantitative Study on Antioxidant Compounds Applied Sciences deep eutectic solvents extraction polyphenols olive leaves |
| title | Extraction of Polyphenols from Olive Leaves Employing Deep Eutectic Solvents: The Application of Chemometrics to a Quantitative Study on Antioxidant Compounds |
| title_full | Extraction of Polyphenols from Olive Leaves Employing Deep Eutectic Solvents: The Application of Chemometrics to a Quantitative Study on Antioxidant Compounds |
| title_fullStr | Extraction of Polyphenols from Olive Leaves Employing Deep Eutectic Solvents: The Application of Chemometrics to a Quantitative Study on Antioxidant Compounds |
| title_full_unstemmed | Extraction of Polyphenols from Olive Leaves Employing Deep Eutectic Solvents: The Application of Chemometrics to a Quantitative Study on Antioxidant Compounds |
| title_short | Extraction of Polyphenols from Olive Leaves Employing Deep Eutectic Solvents: The Application of Chemometrics to a Quantitative Study on Antioxidant Compounds |
| title_sort | extraction of polyphenols from olive leaves employing deep eutectic solvents the application of chemometrics to a quantitative study on antioxidant compounds |
| topic | deep eutectic solvents extraction polyphenols olive leaves |
| url | https://www.mdpi.com/2076-3417/12/2/831 |
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