Exposure of primary osteoblasts to combined magnetic and electric fields induced spatiotemporal endochondral ossification characteristic gene‐ and protein expression profiles
Abstract Purpose Molecular processes in primary osteoblasts were analyzed in response to magnetic and electric field exposure to examine its potential impact on bone healing. Methods Primary osteoblasts were exposed to a combination of a magnetic field and an additional electric field (EFMF) (20 Hz,...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Experimental Orthopaedics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40634-022-00477-9 |
| _version_ | 1797321479469137920 |
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| author | Klaus H. Dittmann Claus Mayer Heribert Stephan Christin Mieth Michael Bonin Beat Lechmann H. Peter Rodemann |
| author_facet | Klaus H. Dittmann Claus Mayer Heribert Stephan Christin Mieth Michael Bonin Beat Lechmann H. Peter Rodemann |
| author_sort | Klaus H. Dittmann |
| collection | DOAJ |
| description | Abstract Purpose Molecular processes in primary osteoblasts were analyzed in response to magnetic and electric field exposure to examine its potential impact on bone healing. Methods Primary osteoblasts were exposed to a combination of a magnetic field and an additional electric field (EFMF) (20 Hz, 700 mV, 5 mT, continuous sinusoids) in vitro. mRNA‐ and protein‐expressions were assessed during a time interval of 21 days and compared with expression data obtained from control osteoblasts. Results We observed an autonomous osteoblast differentiation process in vitro under the chosen cultivation conditions. The initial proliferative phase was characterized by a constitutively high mRNA expression of extracellular matrix proteins. Concurrent EFMF exposure resulted in significanly increased cell proliferation (fold change: 1.25) and reduced mRNA‐expressions of matrix components (0.5–0.75). The following reorganization of the extracellular matrix is prerequisite for matrix mineralization and is characterised by increased Ca2+ deposition (1.44). On molecular level EFMF exposure led to a significant decreased thrombospondin 1 (THBS1) mRNA‐ (0.81) and protein‐ (0.54) expression, which in turn reduced the TGFß1‐dependent mRNA‐ (0.68) and protein‐ (0.5) expression of transforming growth factor beta induced (ßIG‐H3) significantly, an inhibitor of endochondral ossification. Consequently, EFMF exposure stimulated the expression of genes characteristic for endochondral ossification, such as collagen type 10, A1 (1.50), osteopontin (1.50) and acellular communication network factor 3 (NOV) (1.45). Conclusions In vitro exposure of osteoblasts to EFMF supports cell differentiation and induces gene‐ and protein‐expression patterns characteristic for endochondral ossification during bone fracture healing in vivo. |
| first_indexed | 2024-03-08T04:58:12Z |
| format | Article |
| id | doaj.art-22112d7602e04dabbf0178079a7c9691 |
| institution | Directory Open Access Journal |
| issn | 2197-1153 |
| language | English |
| last_indexed | 2024-03-08T04:58:12Z |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Experimental Orthopaedics |
| spelling | doaj.art-22112d7602e04dabbf0178079a7c96912024-02-07T14:50:37ZengWileyJournal of Experimental Orthopaedics2197-11532022-01-0191n/an/a10.1186/s40634-022-00477-9Exposure of primary osteoblasts to combined magnetic and electric fields induced spatiotemporal endochondral ossification characteristic gene‐ and protein expression profilesKlaus H. Dittmann0Claus Mayer1Heribert Stephan2Christin Mieth3Michael Bonin4Beat Lechmann5H. Peter Rodemann6Division of Radiation BiologyDept of Radiation OncologyMedical FacultyEberhard Karls University TübingenRoentgenweg 1172076TuebingenGermanyDivision of Radiation BiologyDept of Radiation OncologyMedical FacultyEberhard Karls University TübingenRoentgenweg 1172076TuebingenGermanyNeue Magnetodyn GmbHHermann‐Oberth‐Str. 985640PutzbrunnGermanyIMGM Laboratories GmbH10x Genomics B.V.LeidenThe NetherlandsIMGM Laboratories GmbH10x Genomics B.V.LeidenThe NetherlandsDePuySynthesSynthes GmbHLuzernstrasse 214528ZuchwilSwitzerlandDivision of Radiation BiologyDept of Radiation OncologyMedical FacultyEberhard Karls University TübingenRoentgenweg 1172076TuebingenGermanyAbstract Purpose Molecular processes in primary osteoblasts were analyzed in response to magnetic and electric field exposure to examine its potential impact on bone healing. Methods Primary osteoblasts were exposed to a combination of a magnetic field and an additional electric field (EFMF) (20 Hz, 700 mV, 5 mT, continuous sinusoids) in vitro. mRNA‐ and protein‐expressions were assessed during a time interval of 21 days and compared with expression data obtained from control osteoblasts. Results We observed an autonomous osteoblast differentiation process in vitro under the chosen cultivation conditions. The initial proliferative phase was characterized by a constitutively high mRNA expression of extracellular matrix proteins. Concurrent EFMF exposure resulted in significanly increased cell proliferation (fold change: 1.25) and reduced mRNA‐expressions of matrix components (0.5–0.75). The following reorganization of the extracellular matrix is prerequisite for matrix mineralization and is characterised by increased Ca2+ deposition (1.44). On molecular level EFMF exposure led to a significant decreased thrombospondin 1 (THBS1) mRNA‐ (0.81) and protein‐ (0.54) expression, which in turn reduced the TGFß1‐dependent mRNA‐ (0.68) and protein‐ (0.5) expression of transforming growth factor beta induced (ßIG‐H3) significantly, an inhibitor of endochondral ossification. Consequently, EFMF exposure stimulated the expression of genes characteristic for endochondral ossification, such as collagen type 10, A1 (1.50), osteopontin (1.50) and acellular communication network factor 3 (NOV) (1.45). Conclusions In vitro exposure of osteoblasts to EFMF supports cell differentiation and induces gene‐ and protein‐expression patterns characteristic for endochondral ossification during bone fracture healing in vivo.https://doi.org/10.1186/s40634-022-00477-9Primary human osteoblastsElectromagnetic fieldWnt‐signaling/TGFß‐signaling |
| spellingShingle | Klaus H. Dittmann Claus Mayer Heribert Stephan Christin Mieth Michael Bonin Beat Lechmann H. Peter Rodemann Exposure of primary osteoblasts to combined magnetic and electric fields induced spatiotemporal endochondral ossification characteristic gene‐ and protein expression profiles Journal of Experimental Orthopaedics Primary human osteoblasts Electromagnetic field Wnt‐signaling/TGFß‐signaling |
| title | Exposure of primary osteoblasts to combined magnetic and electric fields induced spatiotemporal endochondral ossification characteristic gene‐ and protein expression profiles |
| title_full | Exposure of primary osteoblasts to combined magnetic and electric fields induced spatiotemporal endochondral ossification characteristic gene‐ and protein expression profiles |
| title_fullStr | Exposure of primary osteoblasts to combined magnetic and electric fields induced spatiotemporal endochondral ossification characteristic gene‐ and protein expression profiles |
| title_full_unstemmed | Exposure of primary osteoblasts to combined magnetic and electric fields induced spatiotemporal endochondral ossification characteristic gene‐ and protein expression profiles |
| title_short | Exposure of primary osteoblasts to combined magnetic and electric fields induced spatiotemporal endochondral ossification characteristic gene‐ and protein expression profiles |
| title_sort | exposure of primary osteoblasts to combined magnetic and electric fields induced spatiotemporal endochondral ossification characteristic gene and protein expression profiles |
| topic | Primary human osteoblasts Electromagnetic field Wnt‐signaling/TGFß‐signaling |
| url | https://doi.org/10.1186/s40634-022-00477-9 |
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