Turbulence-driven shifts in holobionts and planktonic microbial assemblages in St Peter & St Paul Archipelago, Mid-Atlantic Ridge, Brazil
The aim of this study was to investigate the planktonic and the holobiont Madracis decactis (Scleractinia) microbial diversity along a turbulence-driven upwelling event, in the world´s most isolated tropical island, St Peter and St Paul Archipelago (SPSPA, Brazil). Twenty one metagenomes were obtain...
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Language: | English |
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Frontiers Media S.A.
2015-10-01
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Series: | Frontiers in Microbiology |
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Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01038/full |
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author | Ana Paula B. Moreira Pedro Milet Meirelles Eidy O. Santos Gilberto M. Amado-Filho Ronaldo Bastos Francini-Filho Cristiane C. Thompson Fabiano eThompson |
author_facet | Ana Paula B. Moreira Pedro Milet Meirelles Eidy O. Santos Gilberto M. Amado-Filho Ronaldo Bastos Francini-Filho Cristiane C. Thompson Fabiano eThompson |
author_sort | Ana Paula B. Moreira |
collection | DOAJ |
description | The aim of this study was to investigate the planktonic and the holobiont Madracis decactis (Scleractinia) microbial diversity along a turbulence-driven upwelling event, in the world´s most isolated tropical island, St Peter and St Paul Archipelago (SPSPA, Brazil). Twenty one metagenomes were obtained for seawater (N=12), healthy and bleached holobionts (N=9) before, during and after the episode of high seawater turbulence and upwelling. Microbial assemblages differed between low turbulence-low nutrient (LLR) and high-turbulence-high nutrient (HHR) regimes in seawater. During LLR there was a balance between autotrophy and heterotrophy in the bacterioplankton and the ratio cyanobacteria:heterotrophs ~1 (C:H). Prochlorales, unclassified Alphaproteobacteria and Euryarchaeota were the dominant bacteria and archaea, respectively. Basic metabolisms and cyanobacterial phages characterized the LLR. During HHR C:H << 0.05 and Gammaproteobacteria approximated 50% of the most abundant organisms in seawater. Alteromonadales, Oceanospirillales and Thaumarchaeota were the dominant bacteria and archaea. Prevailing metabolisms were related to membrane transport, virulence, disease and defense. Phages targeting heterotrophs and virulence factor genes characterized HHR. Shifts were also observed in coral microbiomes, according to both annotation–indepent and -dependent methods. HHR bleached corals metagenomes were the most dissimilar and could be distinguished by their di- and tetranucleotides frequencies, Iron Acquision metabolism and virulence genes, such as V. cholerae-related virulence factors. The healthy coral holobiont was shown to be less sensitive to transient seawater-related perturbations than the diseased animals. A conceptual model for the turbulence-induced shifts is put forward. |
first_indexed | 2024-12-19T19:51:17Z |
format | Article |
id | doaj.art-221651b031224c9b97a963f26ed62901 |
institution | Directory Open Access Journal |
issn | 1664-302X |
language | English |
last_indexed | 2024-12-19T19:51:17Z |
publishDate | 2015-10-01 |
publisher | Frontiers Media S.A. |
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series | Frontiers in Microbiology |
spelling | doaj.art-221651b031224c9b97a963f26ed629012022-12-21T20:07:58ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2015-10-01610.3389/fmicb.2015.01038154294Turbulence-driven shifts in holobionts and planktonic microbial assemblages in St Peter & St Paul Archipelago, Mid-Atlantic Ridge, BrazilAna Paula B. Moreira0Pedro Milet Meirelles1Eidy O. Santos2Gilberto M. Amado-Filho3Ronaldo Bastos Francini-Filho4Cristiane C. Thompson5Fabiano eThompson6Federal University of Rio de Janeiro (UFRJ), Institute of BiologyFederal University of Rio de Janeiro (UFRJ), Institute of BiologyState University of West Zone (UEZO)Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ)Federal University of Paraíba (UFPB)Federal University of rio de Janeiro (UFRJ)Federal University of Rio de Janeiro (UFRJ), Institute of BiologyThe aim of this study was to investigate the planktonic and the holobiont Madracis decactis (Scleractinia) microbial diversity along a turbulence-driven upwelling event, in the world´s most isolated tropical island, St Peter and St Paul Archipelago (SPSPA, Brazil). Twenty one metagenomes were obtained for seawater (N=12), healthy and bleached holobionts (N=9) before, during and after the episode of high seawater turbulence and upwelling. Microbial assemblages differed between low turbulence-low nutrient (LLR) and high-turbulence-high nutrient (HHR) regimes in seawater. During LLR there was a balance between autotrophy and heterotrophy in the bacterioplankton and the ratio cyanobacteria:heterotrophs ~1 (C:H). Prochlorales, unclassified Alphaproteobacteria and Euryarchaeota were the dominant bacteria and archaea, respectively. Basic metabolisms and cyanobacterial phages characterized the LLR. During HHR C:H << 0.05 and Gammaproteobacteria approximated 50% of the most abundant organisms in seawater. Alteromonadales, Oceanospirillales and Thaumarchaeota were the dominant bacteria and archaea. Prevailing metabolisms were related to membrane transport, virulence, disease and defense. Phages targeting heterotrophs and virulence factor genes characterized HHR. Shifts were also observed in coral microbiomes, according to both annotation–indepent and -dependent methods. HHR bleached corals metagenomes were the most dissimilar and could be distinguished by their di- and tetranucleotides frequencies, Iron Acquision metabolism and virulence genes, such as V. cholerae-related virulence factors. The healthy coral holobiont was shown to be less sensitive to transient seawater-related perturbations than the diseased animals. A conceptual model for the turbulence-induced shifts is put forward.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01038/fullAtlantic OceanMetagenomicsScleractiniaoceanic islandsMadracis decactis |
spellingShingle | Ana Paula B. Moreira Pedro Milet Meirelles Eidy O. Santos Gilberto M. Amado-Filho Ronaldo Bastos Francini-Filho Cristiane C. Thompson Fabiano eThompson Turbulence-driven shifts in holobionts and planktonic microbial assemblages in St Peter & St Paul Archipelago, Mid-Atlantic Ridge, Brazil Frontiers in Microbiology Atlantic Ocean Metagenomics Scleractinia oceanic islands Madracis decactis |
title | Turbulence-driven shifts in holobionts and planktonic microbial assemblages in St Peter & St Paul Archipelago, Mid-Atlantic Ridge, Brazil |
title_full | Turbulence-driven shifts in holobionts and planktonic microbial assemblages in St Peter & St Paul Archipelago, Mid-Atlantic Ridge, Brazil |
title_fullStr | Turbulence-driven shifts in holobionts and planktonic microbial assemblages in St Peter & St Paul Archipelago, Mid-Atlantic Ridge, Brazil |
title_full_unstemmed | Turbulence-driven shifts in holobionts and planktonic microbial assemblages in St Peter & St Paul Archipelago, Mid-Atlantic Ridge, Brazil |
title_short | Turbulence-driven shifts in holobionts and planktonic microbial assemblages in St Peter & St Paul Archipelago, Mid-Atlantic Ridge, Brazil |
title_sort | turbulence driven shifts in holobionts and planktonic microbial assemblages in st peter amp st paul archipelago mid atlantic ridge brazil |
topic | Atlantic Ocean Metagenomics Scleractinia oceanic islands Madracis decactis |
url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01038/full |
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