VLDL-triglyceride production after alcohol ingestion, studied using [2-13C1] glycerol
We used [2-13C1]glycerol to characterize very low density lipoprotein (VLDL)-triglyceride kinetics and intrahepatic glycerol metabolism in normal men (n = 4) after alcohol (EtOH) ingestion. [2-13C1]glycerol was infused before and after the consumption of 48 g EtOH or a placebo. Three additional subj...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
1998-12-01
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| Series: | Journal of Lipid Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520333113 |
| _version_ | 1818596108220235776 |
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| author | Scott Q. Siler Richard A. Neese Elizabeth J. Parks Marc K. Hellerstein |
| author_facet | Scott Q. Siler Richard A. Neese Elizabeth J. Parks Marc K. Hellerstein |
| author_sort | Scott Q. Siler |
| collection | DOAJ |
| description | We used [2-13C1]glycerol to characterize very low density lipoprotein (VLDL)-triglyceride kinetics and intrahepatic glycerol metabolism in normal men (n = 4) after alcohol (EtOH) ingestion. [2-13C1]glycerol was infused before and after the consumption of 48 g EtOH or a placebo. Three additional subjects also received [1-13C1]acetate in addition to the [2-13C1]glycerol with EtOH treatment. Incorporation of tracer into the glycerol or fatty acid moiety of VLDL-triglyceride was measured by gas chromatography–mass spectrometry and used to calculate VLDL-triglyceride production rates. Intrahepatic triose-phosphate enrichments were also calculated based on mass isotopomer distribution analysis of plasma glucose. There was no difference in VLDL-triglyceride production rates after 48 g EtOH (11.9 ± 3.7 mg/kg/h) or placebo (14.7 ± 3.3 mg/kg/h). The VLDL-triglyceride rate constants calculated by kinetic modeling using the glycerol and acetate tracers in the combined isotope infusion subjects were very closely correlated (r2 = 0.94). The peak VLDL-glycerol enrichments after EtOH were 22.5 ± 3.3% versus 7.6 ± 0.8% after placebo (P < 0.001), while intrahepatic triose-phosphate enrichments were 19.8 ± 1.3% and 13.1 ± 1.2% (P < 0.001), respectively. Moreover, the calculated asymptotic VLDL-glycerol enrichments (representing the hepatic α-glycerol phosphate enrichment) were significantly higher after EtOH than placebo. The higher ratio of VLDL-glycerol to triose-phosphate labeling after EtOH suggests a metabolic block at glycerol 3-phosphate dehydrogenase. We conclude that consumption of 48 g EtOH does not increase VLDL-triglyceride production in normal men but does cause accumulation of tracer in hepatic α-glycerol phosphate.—Siler, S. Q., R. A. Neese, E. J. Parks, and M. K. Hellerstein. VLDL-triglyceride production after alcohol ingestion studied using [2-13C1]glycerol. J. Lipid Res. 1998. 39: 2319–2328. |
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| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-16T11:26:40Z |
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| spelling | doaj.art-22185f040caf4859ac6c394fb6ad24a32022-12-21T22:33:21ZengElsevierJournal of Lipid Research0022-22751998-12-01391223192328VLDL-triglyceride production after alcohol ingestion, studied using [2-13C1] glycerolScott Q. Siler0Richard A. Neese1Elizabeth J. Parks2Marc K. Hellerstein3Department of Nutritional Sciences, 309 Morgan Hall, University of California at Berkeley, CA 94720-3104Department of Nutritional Sciences, 309 Morgan Hall, University of California at Berkeley, CA 94720-3104; Division of Endocrinology and Metabolism, Department of Medicine, University of California, San Francisco, 1001 Potrero Avenue, San Francisco, CA 94110Department of Nutritional Sciences, 309 Morgan Hall, University of California at Berkeley, CA 94720-3104To whom correspondence should be addressed.; Department of Nutritional Sciences, 309 Morgan Hall, University of California at Berkeley, CA 94720-3104; Division of Endocrinology and Metabolism, Department of Medicine, University of California, San Francisco, 1001 Potrero Avenue, San Francisco, CA 94110We used [2-13C1]glycerol to characterize very low density lipoprotein (VLDL)-triglyceride kinetics and intrahepatic glycerol metabolism in normal men (n = 4) after alcohol (EtOH) ingestion. [2-13C1]glycerol was infused before and after the consumption of 48 g EtOH or a placebo. Three additional subjects also received [1-13C1]acetate in addition to the [2-13C1]glycerol with EtOH treatment. Incorporation of tracer into the glycerol or fatty acid moiety of VLDL-triglyceride was measured by gas chromatography–mass spectrometry and used to calculate VLDL-triglyceride production rates. Intrahepatic triose-phosphate enrichments were also calculated based on mass isotopomer distribution analysis of plasma glucose. There was no difference in VLDL-triglyceride production rates after 48 g EtOH (11.9 ± 3.7 mg/kg/h) or placebo (14.7 ± 3.3 mg/kg/h). The VLDL-triglyceride rate constants calculated by kinetic modeling using the glycerol and acetate tracers in the combined isotope infusion subjects were very closely correlated (r2 = 0.94). The peak VLDL-glycerol enrichments after EtOH were 22.5 ± 3.3% versus 7.6 ± 0.8% after placebo (P < 0.001), while intrahepatic triose-phosphate enrichments were 19.8 ± 1.3% and 13.1 ± 1.2% (P < 0.001), respectively. Moreover, the calculated asymptotic VLDL-glycerol enrichments (representing the hepatic α-glycerol phosphate enrichment) were significantly higher after EtOH than placebo. The higher ratio of VLDL-glycerol to triose-phosphate labeling after EtOH suggests a metabolic block at glycerol 3-phosphate dehydrogenase. We conclude that consumption of 48 g EtOH does not increase VLDL-triglyceride production in normal men but does cause accumulation of tracer in hepatic α-glycerol phosphate.—Siler, S. Q., R. A. Neese, E. J. Parks, and M. K. Hellerstein. VLDL-triglyceride production after alcohol ingestion studied using [2-13C1]glycerol. J. Lipid Res. 1998. 39: 2319–2328.http://www.sciencedirect.com/science/article/pii/S0022227520333113MIDAalcoholglycerol phosphatehypertriglyceridemiakinetic modeling |
| spellingShingle | Scott Q. Siler Richard A. Neese Elizabeth J. Parks Marc K. Hellerstein VLDL-triglyceride production after alcohol ingestion, studied using [2-13C1] glycerol Journal of Lipid Research MIDA alcohol glycerol phosphate hypertriglyceridemia kinetic modeling |
| title | VLDL-triglyceride production after alcohol ingestion, studied using [2-13C1] glycerol |
| title_full | VLDL-triglyceride production after alcohol ingestion, studied using [2-13C1] glycerol |
| title_fullStr | VLDL-triglyceride production after alcohol ingestion, studied using [2-13C1] glycerol |
| title_full_unstemmed | VLDL-triglyceride production after alcohol ingestion, studied using [2-13C1] glycerol |
| title_short | VLDL-triglyceride production after alcohol ingestion, studied using [2-13C1] glycerol |
| title_sort | vldl triglyceride production after alcohol ingestion studied using 2 13c1 glycerol |
| topic | MIDA alcohol glycerol phosphate hypertriglyceridemia kinetic modeling |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520333113 |
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