Klebsiella pneumoniae: development of carbapenem resistance due to acquisition of blaNDM-1 during antimicrobial therapy in twin infants with pneumonia
Objectives: To identify the mechanism of in vivo development of carbapenem resistance in Klebsiella pneumoniae.Methods: Seven sequential isolates of K. pneumoniae were obtained from twin infants with pneumonia. Antimicrobial susceptibility testing was performed by agar dilution. Carbapenemases inclu...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01399/full |
| _version_ | 1818434277214257152 |
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| author | Junying eZhu Baixing eDing Xiaogang eXu Demei eZhu fan eYang Hong eZhang Fupin eHu |
| author_facet | Junying eZhu Baixing eDing Xiaogang eXu Demei eZhu fan eYang Hong eZhang Fupin eHu |
| author_sort | Junying eZhu |
| collection | DOAJ |
| description | Objectives: To identify the mechanism of in vivo development of carbapenem resistance in Klebsiella pneumoniae.Methods: Seven sequential isolates of K. pneumoniae were obtained from twin infants with pneumonia. Antimicrobial susceptibility testing was performed by agar dilution. Carbapenemases including KPC and ML were initially screened using phenotypic methods, and carbapenemase-encoding genes were identified by PCR and amplicon sequencing. Plasmids of all clinical isolates and the conjugants of resistant isolates were estimated by S1 pulsed-field gel electrophoresis (PFGE). Molecular typing were conducted by PFGE of XbaI-digested genomic DNA and multilocus sequence typing (MLST). Results: For old brother, the first and third isolates were susceptible to meropenem, whereas the second and fourth isolates were resistant (MICs 16 mg/L). The first and second isolates from the young brother were susceptible to meropenem whereas the third isolate was resistant. All the resistant isolates produced NDM-1 metallo--lactamase. PFGE of XbaI-digested DNA revealed identical patterns for all the 7 isolates. All the isolates had the same sequence type named sequence type 37 (ST37). Conclusions: To our knowledge, this is the first documented case of development of carbapenem resistance in vivo mediated by NDM-1 metallo-b-lactamase in K. pneumoniae during treatment of pneumonia with meropenem. |
| first_indexed | 2024-12-14T16:34:26Z |
| format | Article |
| id | doaj.art-2219fa22fd6041718af0cf465f2acbf9 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-14T16:34:26Z |
| publishDate | 2015-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-2219fa22fd6041718af0cf465f2acbf92022-12-21T22:54:31ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2015-12-01610.3389/fmicb.2015.01399160008Klebsiella pneumoniae: development of carbapenem resistance due to acquisition of blaNDM-1 during antimicrobial therapy in twin infants with pneumoniaJunying eZhu0Baixing eDing1Xiaogang eXu2Demei eZhu3fan eYang4Hong eZhang5Fupin eHu6Children’s Hospital of Shanghai, Jiaotong UniversityHuashan Hospital, Fudan UniversityHuashan Hospital, Fudan UniversityHuashan Hospital, Fudan UniversityHuashan Hospital, Fudan UniversityChildren’s Hospital of Shanghai, Jiaotong UniversityHuashan Hospital, Fudan UniversityObjectives: To identify the mechanism of in vivo development of carbapenem resistance in Klebsiella pneumoniae.Methods: Seven sequential isolates of K. pneumoniae were obtained from twin infants with pneumonia. Antimicrobial susceptibility testing was performed by agar dilution. Carbapenemases including KPC and ML were initially screened using phenotypic methods, and carbapenemase-encoding genes were identified by PCR and amplicon sequencing. Plasmids of all clinical isolates and the conjugants of resistant isolates were estimated by S1 pulsed-field gel electrophoresis (PFGE). Molecular typing were conducted by PFGE of XbaI-digested genomic DNA and multilocus sequence typing (MLST). Results: For old brother, the first and third isolates were susceptible to meropenem, whereas the second and fourth isolates were resistant (MICs 16 mg/L). The first and second isolates from the young brother were susceptible to meropenem whereas the third isolate was resistant. All the resistant isolates produced NDM-1 metallo--lactamase. PFGE of XbaI-digested DNA revealed identical patterns for all the 7 isolates. All the isolates had the same sequence type named sequence type 37 (ST37). Conclusions: To our knowledge, this is the first documented case of development of carbapenem resistance in vivo mediated by NDM-1 metallo-b-lactamase in K. pneumoniae during treatment of pneumonia with meropenem.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01399/fullin vivoKlebsiella pneumoniae;NDM-1 Metallo--Lactamase;Development of Carbapenem Resistance;Meropenem; |
| spellingShingle | Junying eZhu Baixing eDing Xiaogang eXu Demei eZhu fan eYang Hong eZhang Fupin eHu Klebsiella pneumoniae: development of carbapenem resistance due to acquisition of blaNDM-1 during antimicrobial therapy in twin infants with pneumonia Frontiers in Microbiology in vivo Klebsiella pneumoniae; NDM-1 Metallo--Lactamase; Development of Carbapenem Resistance; Meropenem; |
| title | Klebsiella pneumoniae: development of carbapenem resistance due to acquisition of blaNDM-1 during antimicrobial therapy in twin infants with pneumonia |
| title_full | Klebsiella pneumoniae: development of carbapenem resistance due to acquisition of blaNDM-1 during antimicrobial therapy in twin infants with pneumonia |
| title_fullStr | Klebsiella pneumoniae: development of carbapenem resistance due to acquisition of blaNDM-1 during antimicrobial therapy in twin infants with pneumonia |
| title_full_unstemmed | Klebsiella pneumoniae: development of carbapenem resistance due to acquisition of blaNDM-1 during antimicrobial therapy in twin infants with pneumonia |
| title_short | Klebsiella pneumoniae: development of carbapenem resistance due to acquisition of blaNDM-1 during antimicrobial therapy in twin infants with pneumonia |
| title_sort | klebsiella pneumoniae development of carbapenem resistance due to acquisition of blandm 1 during antimicrobial therapy in twin infants with pneumonia |
| topic | in vivo Klebsiella pneumoniae; NDM-1 Metallo--Lactamase; Development of Carbapenem Resistance; Meropenem; |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01399/full |
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