The zebrafish mutant dreammist implicates sodium homeostasis in sleep regulation
Sleep is a nearly universal feature of animal behaviour, yet many of the molecular, genetic, and neuronal substrates that orchestrate sleep/wake transitions lie undiscovered. Employing a viral insertion sleep screen in larval zebrafish, we identified a novel gene, dreammist (dmist), whose loss resul...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2023-08-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/87521 |
| _version_ | 1797752180951744512 |
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| author | Ida L Barlow Eirinn Mackay Emily Wheater Aimee Goel Sumi Lim Steve Zimmerman Ian Woods David A Prober Jason Rihel |
| author_facet | Ida L Barlow Eirinn Mackay Emily Wheater Aimee Goel Sumi Lim Steve Zimmerman Ian Woods David A Prober Jason Rihel |
| author_sort | Ida L Barlow |
| collection | DOAJ |
| description | Sleep is a nearly universal feature of animal behaviour, yet many of the molecular, genetic, and neuronal substrates that orchestrate sleep/wake transitions lie undiscovered. Employing a viral insertion sleep screen in larval zebrafish, we identified a novel gene, dreammist (dmist), whose loss results in behavioural hyperactivity and reduced sleep at night. The neuronally expressed dmist gene is conserved across vertebrates and encodes a small single-pass transmembrane protein that is structurally similar to the Na+,K+-ATPase regulator, FXYD1/Phospholemman. Disruption of either fxyd1 or atp1a3a, a Na+,K+-ATPase alpha-3 subunit associated with several heritable movement disorders in humans, led to decreased night-time sleep. Since atpa1a3a and dmist mutants have elevated intracellular Na+ levels and non-additive effects on sleep amount at night, we propose that Dmist-dependent enhancement of Na+ pump function modulates neuronal excitability to maintain normal sleep behaviour. |
| first_indexed | 2024-03-12T17:00:34Z |
| format | Article |
| id | doaj.art-221b77ce3f6641329cfae8555a7e436b |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-03-12T17:00:34Z |
| publishDate | 2023-08-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-221b77ce3f6641329cfae8555a7e436b2023-08-07T14:09:47ZengeLife Sciences Publications LtdeLife2050-084X2023-08-011210.7554/eLife.87521The zebrafish mutant dreammist implicates sodium homeostasis in sleep regulationIda L Barlow0Eirinn Mackay1Emily Wheater2Aimee Goel3Sumi Lim4Steve Zimmerman5Ian Woods6David A Prober7https://orcid.org/0000-0002-7371-4675Jason Rihel8https://orcid.org/0000-0003-4067-2066Department of Cell and Developmental Biology, University College London, London, United KingdomDepartment of Cell and Developmental Biology, University College London, London, United KingdomDepartment of Cell and Developmental Biology, University College London, London, United KingdomDepartment of Cell and Developmental Biology, University College London, London, United KingdomDepartment of Cell and Developmental Biology, University College London, London, United KingdomDepartment of Molecular and Cellular Biology, Harvard University, Cambridge, United StatesIthaca College, New York, United StatesDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, United StatesDepartment of Cell and Developmental Biology, University College London, London, United KingdomSleep is a nearly universal feature of animal behaviour, yet many of the molecular, genetic, and neuronal substrates that orchestrate sleep/wake transitions lie undiscovered. Employing a viral insertion sleep screen in larval zebrafish, we identified a novel gene, dreammist (dmist), whose loss results in behavioural hyperactivity and reduced sleep at night. The neuronally expressed dmist gene is conserved across vertebrates and encodes a small single-pass transmembrane protein that is structurally similar to the Na+,K+-ATPase regulator, FXYD1/Phospholemman. Disruption of either fxyd1 or atp1a3a, a Na+,K+-ATPase alpha-3 subunit associated with several heritable movement disorders in humans, led to decreased night-time sleep. Since atpa1a3a and dmist mutants have elevated intracellular Na+ levels and non-additive effects on sleep amount at night, we propose that Dmist-dependent enhancement of Na+ pump function modulates neuronal excitability to maintain normal sleep behaviour.https://elifesciences.org/articles/87521sleepsodium-potassium pumpsleep homeostasiszebrafishgenetic screen |
| spellingShingle | Ida L Barlow Eirinn Mackay Emily Wheater Aimee Goel Sumi Lim Steve Zimmerman Ian Woods David A Prober Jason Rihel The zebrafish mutant dreammist implicates sodium homeostasis in sleep regulation eLife sleep sodium-potassium pump sleep homeostasis zebrafish genetic screen |
| title | The zebrafish mutant dreammist implicates sodium homeostasis in sleep regulation |
| title_full | The zebrafish mutant dreammist implicates sodium homeostasis in sleep regulation |
| title_fullStr | The zebrafish mutant dreammist implicates sodium homeostasis in sleep regulation |
| title_full_unstemmed | The zebrafish mutant dreammist implicates sodium homeostasis in sleep regulation |
| title_short | The zebrafish mutant dreammist implicates sodium homeostasis in sleep regulation |
| title_sort | zebrafish mutant dreammist implicates sodium homeostasis in sleep regulation |
| topic | sleep sodium-potassium pump sleep homeostasis zebrafish genetic screen |
| url | https://elifesciences.org/articles/87521 |
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