Fluorescence lifetime imaging through scattering media
Abstract Fluorescence lifetime determination has proven to be useful, e.g. identification of molecules, quantitative estimation of species concentration and determination of temperatures. Lifetime determination of exponentially decaying signals is challenging if signals of different decay rates are...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-30055-7 |
| _version_ | 1797864610889465856 |
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| author | Sebastian Nilsson Elias Kristensson Marcus Aldén Joakim Bood Andreas Ehn |
| author_facet | Sebastian Nilsson Elias Kristensson Marcus Aldén Joakim Bood Andreas Ehn |
| author_sort | Sebastian Nilsson |
| collection | DOAJ |
| description | Abstract Fluorescence lifetime determination has proven to be useful, e.g. identification of molecules, quantitative estimation of species concentration and determination of temperatures. Lifetime determination of exponentially decaying signals is challenging if signals of different decay rates are being mixed, resulting in erroneous results. Such issues occur when the contrast of the measurement object is low, which can be limiting in applied measurements due to spurious light scattering. A solution is presented here where structured illumination is used to enhance image contrast in fluorescence lifetime wide-field imaging. Lifetime imaging determination was carried out using Dual Imaging Modeling Evaluation (DIME), and spatial lock-in analysis was used for removing spurious scattered signal to enable fluorescence lifetime imaging through scattering media. |
| first_indexed | 2024-04-09T22:55:49Z |
| format | Article |
| id | doaj.art-221fa221b22d4863bfb7878d9f188f03 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T22:55:49Z |
| publishDate | 2023-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-221fa221b22d4863bfb7878d9f188f032023-03-22T11:16:18ZengNature PortfolioScientific Reports2045-23222023-02-011311910.1038/s41598-023-30055-7Fluorescence lifetime imaging through scattering mediaSebastian Nilsson0Elias Kristensson1Marcus Aldén2Joakim Bood3Andreas Ehn4Combustion Physics, Department of Physics, Faculty of Engineering, Lund UniversityCombustion Physics, Department of Physics, Faculty of Engineering, Lund UniversityCombustion Physics, Department of Physics, Faculty of Engineering, Lund UniversityCombustion Physics, Department of Physics, Faculty of Engineering, Lund UniversityCombustion Physics, Department of Physics, Faculty of Engineering, Lund UniversityAbstract Fluorescence lifetime determination has proven to be useful, e.g. identification of molecules, quantitative estimation of species concentration and determination of temperatures. Lifetime determination of exponentially decaying signals is challenging if signals of different decay rates are being mixed, resulting in erroneous results. Such issues occur when the contrast of the measurement object is low, which can be limiting in applied measurements due to spurious light scattering. A solution is presented here where structured illumination is used to enhance image contrast in fluorescence lifetime wide-field imaging. Lifetime imaging determination was carried out using Dual Imaging Modeling Evaluation (DIME), and spatial lock-in analysis was used for removing spurious scattered signal to enable fluorescence lifetime imaging through scattering media.https://doi.org/10.1038/s41598-023-30055-7 |
| spellingShingle | Sebastian Nilsson Elias Kristensson Marcus Aldén Joakim Bood Andreas Ehn Fluorescence lifetime imaging through scattering media Scientific Reports |
| title | Fluorescence lifetime imaging through scattering media |
| title_full | Fluorescence lifetime imaging through scattering media |
| title_fullStr | Fluorescence lifetime imaging through scattering media |
| title_full_unstemmed | Fluorescence lifetime imaging through scattering media |
| title_short | Fluorescence lifetime imaging through scattering media |
| title_sort | fluorescence lifetime imaging through scattering media |
| url | https://doi.org/10.1038/s41598-023-30055-7 |
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